First Record of Identification of RPMS1 Gene Variations in Vietnamese Nasopharyngeal Carcinoma Patients

Abstract

Epstein-Barr Virus (EBV) infection and EBV genes' variation are considered as the etiological factors contributed to Naso-Pharyngeal Carcinoma (NPC). In the latent EBV stage, not only the latent membrane proteins but also RPMS1 expression has been confirmed in all EBV-associated tumors. In Vietnam, an Asian country with the high incidence, mortality rate of NPC, had limit research on the RMPS1 gene variation. Therefore, the objects of current study were to identify the pattern of RMPS1 variations in Vietnamese NPC patients for its value further applied in NPC patients. In this study, thirty NPC biopsy samples and thirty non-cancerous swab specimens were collected from local patients, analyzed by PCR, sequencing and compared to previous B95-8 sequence. As the results, the strongly association between the detection of RPSM1 gene and NPC incidence in Vietnamese NPC patients was determined. Additionally, the RMPS1 gene variants, including wild-type, RMPS1-B, RMPS1-C, RMPS1-C*, were identified. Among them, RMPS1-C/C* was preferential in Vietnamese nasopharyngeal cancer. In conclusion, those data is the first dataset on the polymorphism in the RPMS1 gene in Vietnamese NPC patients, and could be utilized as a promising biomarker for prognosis, diagnosis and therapy for NPC based on the EBV gene variations.in reached to 4,931 cases (ASR = 5.4/100,000) and deaths was 2,885 cases (ASR = 3.3/100,000) in Vietnamese population 3 . For the past few years, many studies have been demonstrated that the major etiological factors proposed for NPC pathogenesis, including Epstein-Barr virus (EBV) infection, genetics/or epigenetic changes and environmental factor 4-8 . In the latent EBV stage, additionally to the latent membrane proteins (LMP-1, LMP-2), and small noncoding RNAs (EBERs), as