First Record of a Spotted Fever GroupRickettsiasp. andTheileria annulatainHyalomma dromedarii(Acari: Ixodidae) Ticks in the United Arab Emirates

Al-Deeb, Mohammad Ali; Muzaffar, Sabir Bin; Abu-Zeid, Yousif A.; Enan, Mohamed R.; Karim, Shahid

doi:10.1653/024.098.0123

Cited by 36 publications

(32 citation statements)

References 15 publications

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“…Moreover, the previous reports detected the presence R. aeschlimannii , R. africae , and other Rickettsia spp. by PCR in H. dromedarii from Egypt [3,5,6] and in other adjacent countries as Tunisia, Algeria, Israel and the United Arab Emirates [82-86]. Subsequently, this work confirms the role of both tick spp.…”

Section: Discussionsupporting

confidence: 71%

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Abdullah¹,

El-Molla²,

Salib³

et al. 2016

Vet World

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Aim:Rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. The dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii play important roles as vectors and reservoirs of Rickettsiae. The aim of this study was to determine the prevalence of Rickettsiae in ixodid ticks species infesting dogs and camels in Egypt, in addition to, the morphological and molecular identification of R. sanguineus and H. dromedarii.Materials and Methods:A total of 601 and 104 of ticks’ specimens were collected from dogs and camels, respectively, in Cairo, Giza and Sinai provinces. Hemolymph staining technique and OmpA and gltA genes amplification were performed to estimate the prevalence rate of Rickettsiae in ticks. For morphological identification of tick species, light microscope (LM) and scanning electron microscope (SEM) were used. In addition to the phylogenetic analyses of 18S rDNA, Second internal transcript spacer, 12S rDNA, cytochrome c oxidase subunit-1, and 16S rDNA were performed for molecular identification of two tick species.Results:The prevalence rate of Rickettsiae in ticks was 11.6% using hemolymph staining technique and 6.17% by OmpA and gltA genes amplification. Morphological identification revealed that 100% of dogs were infested by R. sanguineus while 91.9% of camels had been infested by H. dromedarii. The phylogenetic analyses of five DNA markers confirmed morphological identification by LM and SEM. The two tick species sequences analyses proved 96-100% sequences identities when compared with the reference data in Genbank records.Conclusion:The present studies confirm the suitability of mitochondrial DNA markers for reliable identification of ticks at both intra- and inter-species level over the nuclear ones. In addition to, the detection of Rickettsiae in both ticks’ species and establishment of the phylogenetic status of R. sanguineus and H. dromedarii would be useful in understanding the epidemiology of ticks and tick borne rickettsioses in Egypt.

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Section: Discussionsupporting

confidence: 71%

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Abdullah¹,

El-Molla²,

Salib³

et al. 2016

Vet World

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“…have been detected in camel blood serum with prevalences of up to 83% [21,58] and R. aeschlimannii, R. africae, R. sibirica mongolitimonae and Rickettsia sp. have been identified in several tick species collected from camels [20,[59][60][61][62][63][64][65][66]. Despite this, there are no reports on diseases in camels caused by these organisms.…”

Section: Discussionmentioning

confidence: 99%

Vector-borne bacteria in blood of camels in Iran: New data and literature review

Sazmand

Harl

Eigner

et al. 2019

Comparative Immunology, Microbiology and Infectious Diseases

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A B S T R A C TDespite close association between camels and humans, molecular based studies on vector-borne pathogens infecting camels are scarce compared to other animals in Iran. The current study was carried out to investigate the occurrence of vector-borne bacteria in the blood of dromedaries by molecular tools. A total of 200 peripheral blood samples were collected from apparently healthy animals. Microscopic examination was performed on Giemsa-stained blood smears, and drops of blood were spotted on Whatman FTA ® cards for molecular analyses. Genomic DNA was extracted from the cards, and PCR amplification followed by sequencing of positive samples was carried out for the detection of Anaplasmataceae, spotted fever group (SFG) rickettsiae, Bartonella spp. and Borrelia spp. Intra-cytic forms of any blood pathogens could not be detected by light microscopy. PCR results revealed 30 animals (15%) to be infected with Anaplasmataceae bacteria. Analyses of sequences revealed a strain of Anaplasma sp. identical to Candidatus Anaplasma camelii isolated from camels, cattle and deer in Asia and Africa. Neither SFG rickettsiae, nor Borrelia or Bartonella species were found. Further studies for determining epidemiological role of camels and its zoonotic potential are recommended. This paper reviews the current knowledge on camels' tickborne bacteria including microscopy, serology and molecular studies.confirmed by DNA sequencing in camels are Candidatus Anaplasma camelii (genetically close to A. platys), A. phagocytophilum and A. ovis [10][11][12][13][14][15][16][17][18]. Clinical signs of natural infections are described as fever, pale and icteric conjunctiva suggestive of anaemia, dullness, anorexia, diarrhoea, loss of appetite, emaciation, coughing, lacrimation, rough hair coat, abortion, and/or infertility [7,19]. Intramuscular administration of oxytetracycline at 20 mg/kg as specific therapy and injectable B-complex, iron, folic acid and hydroxycobalamin as supportive therapy is the recommended therapeutic regimen [7,19]. Infection with and antibodies against several species of Rickettsia [20,21], as well as infection with Bartonella [22] and Borrelia [23,24] have been also documented.According to official estimates, around 183,900 camels live in Iran [25]. Given the growing scientific and public health interest in camels, we investigated the occurrence of selected vector-borne bacteria;https://doi.

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“…Ticks of Hyalomma genus were considered as potential vectors of Rickettsia spp., especially H. dromedarii (Abdullah et al, ; Al‐Deeb, Bin Muzaffar, Abu‐Zeid, Rizk Enan, & Shahid, ), H. impeltatum , H. excavatum (Ereqat et al, ; Kleinerman et al, ), as well as H. impressum, and H. marginatum rufipes (Djerbouh et al, ).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and phylogeny of spotted fever group Rickettsia in camels ( Camelus dromedarius ) and their infesting ticks from Tunisia

Selmi

Saïd

Yahia

et al. 2019

Transbound Emerg Dis

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Rickettsia species are adapted to a wide range of specific animal hosts. Camels (Camelus dromedarius) have been identified as a carrier of various zoonotic pathogens and became a focus of growing public health interest. This study reported the occurrence of rickettsial infection in camels and infesting ticks from five Tunisian governorates. Based on ompB PCR, eight out of 293 camels (2.7%) were found to be infected with Rickettsia spp. Furthermore, 13 tick specimens of Hyalomma impeltatum (10.4%) and 9 of H. dromedarii (8.0%) harboured DNA of Rickettsia bacteria with an overall prevalence rate of 9.2% (22/237). Molecular prevalence of Rickettsia infection varied significantly according to tick infestation for camels and among tick genders. Five rickettsial species, showing a potential public health interest, were revealed by sequencing. Based on ompB partial sequences, five species were identified corresponding to R. aeschlimannii, R. monacensis, R. helvetica and R. massiliae in camels and to R. africae, R. aeschlimannii, R. monacensis and R. helvetica in ticks. Based on ompA typing, three species were revealed corresponding to R. africae and R. monacensis in camels and to R. africae, R. aeschlimannii and R. monacensis in ticks. This is the first report consolidating the hypothesis that camels may serve as potential hosts for Rickettsia spp. and Hyalomma spp. ticks as possible vectors in arid and Saharan areas of Tunisia. The present data highlight the importance of preventive measures and survey that must be implemented in camel herds in order to limit the spread of these vector‐borne bacteria to animals and humans.

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First Record of a Spotted Fever GroupRickettsiasp. andTheileria annulatainHyalomma dromedarii(Acari: Ixodidae) Ticks in the United Arab Emirates

Cited by 36 publications

References 15 publications

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Vector-borne bacteria in blood of camels in Iran: New data and literature review

Molecular epidemiology and phylogeny of spotted fever group Rickettsia in camels ( Camelus dromedarius ) and their infesting ticks from Tunisia

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