Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Abdullah, Hend H.A.M.; El-Molla, Amal; Salib, Fayez Awadalla; Allam, Nesreen A. T.; Ghazy, Alaa A.; Abdel-Shafy, Sobhy

doi:10.14202/vetworld.2016.1087-1101

Cited by 49 publications

(45 citation statements)

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“…However, the detection of SFG rickettsiae in the present study indicated the possibility for these pathogens to be present in dogs and horses in Egypt, and the importance of these domestic animals as potential infection amplifiers which play a more dominant role in the persistence of rickettsiae in the nature than previously thought [2,7]. Hence, the detection of tick-borne rickettsiae in dogs and their ticks in the previous studies indicated that both the animal and human populations in Egypt are at risk for these pathogens [19]. In addition to, Rh.…”

Section: Primer Amplifying Sequence Of Rickettsiae Wassupporting

confidence: 57%

“…The PCR reactions were performed in a PTC-100™ thermal cycler (MJ Research Inc., USA) under complete aseptic condition. The protocol of the reactions was applied according to Abdullah et al [19]. PCR products were visualized by electrophoreses in 1.5% agarose stained with ethidium bromide.…”

Section: Screening Of Rickettsiae By Pcr Using Ompa Genementioning

confidence: 99%

“…In Egypt, a few studies were conducted on the diagnoses of Spotted Fever Group rickettsiae (SFG) in ixodid ticks. Two SFG species, Rickettsia africae and Rickettsia aeschlimanni were recorded in Hyalomma spp collected from camels, and R. africae was only detected in the camel host [18][19][20]. Knowledge about rickettsiae in dogs and horses are still lack.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

et al. 2019

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Section: Primer Amplifying Sequence Of Rickettsiae Wassupporting

confidence: 57%

Section: Screening Of Rickettsiae By Pcr Using Ompa Genementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

et al. 2019

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“…A total of 113 camels were examined for the presence of ticks on different parts of their bodies according to Abdullah et al . [ 25 ] from Cairo, Giza, and Sinai in Egypt during the period from June to August 2017. Blood samples with ethylenediaminetetraacetic acid (EDTA) (5 ml each animal) were collected from jugular veins and were stored at −20°C for molecular purposes.…”

Section: Methodsmentioning

confidence: 99%

Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels

Abdullah¹,

Shanawany²,

Abdel-Shafy³

et al. 2018

Vet World

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Background and Aim:Q fever Coxiella burnetii is a worldwide zoonotic disease, and C. burnetii was detected in mammals and ticks. Ticks play an important role in the spread of C. burnetii in the environment. Therefore, the aims of this study were to detect Q fever C. burnetii in camels and ixodid ticks by molecular tools and identification of Hyalomma dromedarii and Hyalomma excavatum using molecular and immunological assays.Materials and Methods:A total of 113 blood samples from camels and 190 adult ticks were investigated for the infection with C. burnetii by polymerase chain reaction (PCR) and sequencing the targeting IS30A spacer. The two tick species H. dromedarii and H. excavatum were characterized molecularly by PCR and sequencing of 16S ribosomal RNA (16S rRNA) and cytochrome oxidase subunit-1 (CO1) genes and immunologically by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot.Results:A total of 52 camels (46%) were positive for Q fever infection. Only 10 adult ticks of H. dromedarii were infected with C. burnetii. The IS30A sequence was around 200 bp in length for C. burnetii in H. dromedarii ticks with a similarity of 99% when compared with reference data in GenBank records. The length of 16S rDNA and CO1 was 440 and 850 bp, respectively, for both H. dromedarii and H. excavatum. The phylogenetic status of H. dromedarii was distant from that of H. excavatum. SDS-PAGE revealed seven different bands in the adult antigens of either H. dromedarii or H. excavatum with molecular weights ranged from 132.9 to 17.7 KDa. In western blot analyses, the sera obtained from either infested camel by H. dromedarii or infested cattle by H. excavatum recognized four immunogenic bands (100.7, 49.7, 43.9, and 39.6 kDa) in H. dromedarii antigen. However, the infested camel sera identified two immunogenic bands (117 and 61.4 kDa) in H. excavatum antigen. Furthermore, the sera collected from cattle infested by H. excavatum recognized three immunogenic bands (61.4, 47.3, and 35 kDa) in H. excavatum antigen.Conclusion:Molecular analyses indicated that both camels and ticks could be sources for infection of animals and humans with Q fever. Furthermore, the molecular analyses are more accurate tools for discriminating H. dromedarii and H. excavatum than immunological tools.

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“…Standard PCR was applied to confirm MALDI-TOF identification. Mitochondrial genes (CO1 and 16S rRNA gene) sequencing was used for the identification of flies and tick species through DNA automated thermal cycler (Applied Biosystem, Paris, France) under the same condition as previously described (Table 1) [48,49]. PCR products were purified and sequenced as mentioned earlier [48].…”

Section: -Molecular Identification Of Vectorsmentioning

confidence: 99%

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.

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Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Cited by 49 publications

References 90 publications

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

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