First Practical Colorimetric Assay of Lithium in Serum

Chapoteau, Eddy; Czech, B.; Zazulak, Wolodymyr; Kumar, Anand

doi:10.1093/clinchem/38.9.1654

Cited by 32 publications

(15 citation statements)

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“…Li levels were determined in serum samples of Li groups animals (aCSF + Li and OUA + Li) at the 7th, 9th, and 14th days according to Chapoteau and coworkers 28 .…”

Section: Methodsmentioning

confidence: 99%

Validation of the animal model of bipolar disorder induced by Ouabain: face, construct and predictive perspectives

Valvassori¹,

Dal‐Pont²,

Resende³

et al. 2019

Transl Psychiatry

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A particular challenge in the development of a bipolar disorder (BD) model in animals is the complicated clinical course of the condition, characterized by manic, depressive and mixed mood episodes. Ouabain (OUA) is an inhibitor of Na + /K + -ATPase enzyme. Intracerebroventricular (ICV) injection of this drug in rats has been regarded a proper model to study BD by mimic specific manic symptoms, which are reversed by lithium (Li), an important mood stabilizer drug. However, further validation of this experimental approach is required to characterize it as an animal model of BD, including depressive-like behaviors. The present study aimed to assess manic- and depressive-like behaviors, potential alteration in the hypothalamic-pituitary-adrenal (HPA) system and oxidative stress parameters after a single OUA ICV administration in adult male Wistar rats. Moreover, we evaluated Li effects in this experimental setting. Data show that OUA ICV administration could constitute a suitable model for BD since the injection of the drug triggered manic- and depressive-like behaviors in the same animal. Additionally, the OUA model mimics significant physiological and neurochemical alterations detected in BD patients, including an increase in oxidative stress and change in HPA axis. Our findings suggest that decreased Na + /K + -ATPase activity detected in bipolar patients may be linked to increased secretion of glucocorticoid hormones and oxidative damage, leading to the marked behavioral swings. The Li administration mitigated these pathological changes in the rats. The proposed OUA model is regarded as suitable to simulate BD by complying with all validities required to a proper animal model of the psychiatric disorder.

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“…Li levels were determined in serum samples of Li groups animals (aCSF + Li and OUA + Li) at the 7th, 9th, and 14th days according to Chapoteau and coworkers 28 .…”

Section: Methodsmentioning

confidence: 99%

Validation of the animal model of bipolar disorder induced by Ouabain: face, construct and predictive perspectives

Valvassori¹,

Dal‐Pont²,

Resende³

et al. 2019

Transl Psychiatry

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show abstract

“…Trunk blood was collected by decapitation and serum separated by centrifugation. Concentration of lithium was determined by a colorimetric method [33] in NordLab Oulu (Oulu, Finland) and that of lamotrigine by a liquid chromatography after solid-phase extraction [34] in Rinnekoti-Foundation Laboratory (Espoo, Finland).…”

Section: Methodsmentioning

confidence: 99%

Chronic Treatment with Mood-Stabilizers Attenuates Abnormal Hyperlocomotion of GluA1-Subunit Deficient Mice

et al. 2014

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Abnormal excitatory glutamate neurotransmission and plasticity have been implicated in schizophrenia and affective disorders. Gria1−/− mice lacking GluA1 subunit (encoded by Gria1 gene) of AMPA-type glutamate receptor show robust novelty-induced hyperactivity, social deficits and heightened approach features, suggesting that they could be used to test for anti-manic activity of drugs. Here, we tested the efficacy of chronic treatment with established anti-manic drugs on behavioural properties of the Gria1−/− mice. The mice received standard mood stabilizers (lithium and valproate) and novel ones (topiramate and lamotrigine, used more as anticonvulsants) as supplements in rodent chow for at least 4 weeks. All drugs attenuated novelty-induced locomotor hyperactivity of the Gria1−/− mice, especially by promoting the habituation, while none of them attenuated 2-mg/kg amphetamine-induced hyperactivity as compared to control diet. Treatment with lithium and valproate reversed the elevated exploratory activity of Gria1−/− mice. Valproate treatment also reduced struggling behaviour in tail suspension test and restored reciprocally-initiated social contacts of Gria1−/− mice to the level shown by the wild-type Gria1+/+ mice. Gria1−/− mice consumed slightly more sucrose during intermittent sucrose exposure than the wild-types, but ran similar distances on running wheels. These behaviours were not consistently affected by lithium and valproate in the Gria1−/− mice. The efficacy of various anti-manic drug treatments on novelty-induced hyperactivity suggests that the Gria1−/− mouse line can be utilized in screening for new therapeutics.

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“…Determination of LiCl concentrations in sera. Mouse sera were collected, and the concentrations of LiCl were determined using a colorimetric assay (34) and then detected using a Dimension RxL Max Integrated Chemistry System 50/60 Hz (Siemens Healthcare Diagnostics, Germany). The average concentration in each group was generated by examination of sera from six mice and was expressed as the mean Ϯ standard deviation.…”

Section: Methodsmentioning

confidence: 99%

Protection against Klebsiella pneumoniae Using Lithium Chloride in an Intragastric Infection Model

Tsao

Kuo

Chiu

et al. 2015

Antimicrob Agents Chemother

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Intragastric Klebsiella pneumoniae infections of mice can cause liver abscesses, necrosis of liver tissues, and bacteremia. Lithium chloride, a widely prescribed drug for bipolar mood disorder, has been reported to possess anti-inflammatory properties. Using an intragastric infection model, the effects of LiCl on K. pneumoniae infections were examined. Providing mice with drinking water containing LiCl immediately after infection protected them from K. pneumoniae-induced death and liver injuries, such as necrosis of liver tissues, as well as increasing blood levels of aspartate aminotransferase and alanine aminotransferase, in a dosedependent manner. LiCl administered as late as 24 h postinfection still provided protection. Monitoring of the LiCl concentrations in the sera of K. pneumoniae-infected mice showed that approximately 0.33 mM LiCl was the most effective dose for protecting mice against infections, which is lower than the clinically toxic dose of LiCl. Surveys of bacterial counts and cytokine expression levels in LiCl-treated mice revealed that both were effectively inhibited in blood and liver tissues. Using in vitro assays, we found that LiCl (5 M to 1 mM) did not directly interfere with the growth of K. pneumoniae but made K. pneumoniae cells lose the mucoid phenotype and become more susceptible to macrophage killing. Furthermore, low doses of LiCl also partially enhanced the bactericidal activity of macrophages. Taken together, these data suggest that LiCl is an alternative therapeutic agent for K. pneumoniae-induced liver infections.

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First Practical Colorimetric Assay of Lithium in Serum

Cited by 32 publications

References 0 publications

Validation of the animal model of bipolar disorder induced by Ouabain: face, construct and predictive perspectives

Validation of the animal model of bipolar disorder induced by Ouabain: face, construct and predictive perspectives

Chronic Treatment with Mood-Stabilizers Attenuates Abnormal Hyperlocomotion of GluA1-Subunit Deficient Mice

Protection against Klebsiella pneumoniae Using Lithium Chloride in an Intragastric Infection Model

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