First phenotypic description of Fasciola hepatica/Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt

Periago, María Victoria; Valero, M. Adela; Sayed, Mabrouk El; Ashrafi, Keyhan; Wakeel, Aly El; Mohamed, M.Y.; Desquesnes, Marc; Curtale, Filippo; Mas‐Coma, Santiago

doi:10.1016/j.meegid.2007.10.001

Cited by 127 publications

(99 citation statements)

References 26 publications

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“…1 In Egypt, intermediate hybrid forms exist together with Fasciola hepatica and F. gigantica. 2 The wide range of egg-size variability makes it difficult to determine species from eggs found in stool analysis. 3,4 Fascioliasis has been endemic in certain villages in the Nile Delta, but overall prevalence in Egypt is unknown because reports show wide variations in infection rates.…”

Section: Introductionmentioning

confidence: 99%

Human Fascioliasis: A Re-emerging Disease in Upper Egypt

Mekky¹,

Tolba²,

Abdelmalek³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. In recent years, the number of humans infected with Fasciola has risen rapidly. Diagnosis is based mainly on detection of eggs in stool analysis. The rate of infection in Egypt is unknown. In this retrospective study, we describe 23 cases of hepatic fascioliasis, and only 2 of these cases showed eggs in stools. The symptoms of infection, such as pyrexia of unknown origin, epigastric pain, and abdominal distension, were suggestive. Imaging techniques, including abdominal ultrasonography and computed tomography, were very helpful in detecting hepatic changes. An indirect hemagglutination assay proved to be of value for diagnosis. Treatment using a 2-day triclabendazole regimen cured the infection and signs of hepatic involvement disappeared. Combining both imaging techniques and laboratory tests is essential for diagnosis of fascioliasis in the early stage.

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Section: Introductionmentioning

confidence: 99%

Human Fascioliasis: A Re-emerging Disease in Upper Egypt

Mekky¹,

Tolba²,

Abdelmalek³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…Groups based on maximum and minimum values of the morphological measurements of Fasciola hepatica, Fasciola gigantica or Fasciola sp. (intermediate/hybrid forms) have been proposed for populations from Egypt and Iran [18], whereas, some authors have outlined some useful morphometric descriptions for the specific differentiation of the two species [21]. The basis of grouping was initially performed according to BL/ BW, and, secondarily, according to Distance between ventral sucker and posterior end of the body (VS-P).…”

Section: Discussionmentioning

confidence: 99%

“…Flukes were extensively washed with distilled water and identified morphologically as Fasciola gigantica according to existing keys (body length (BL), body width (BW), body length to width ration (BL/BW); Cone Length (CL); appearance of body sides and shoulder characteristics) as described by other authors [17,18] and stored at -70°C until extraction of genomic DNA. Total genomic DNA extraction: Body portions (the conical anterior end) from individual trematodes were each placed in 200 µl of sterile lysis buffer, containing 0.4 M NaCl, 10 mM Tris-HCl (pH 8) and 2 mM EDTA (pH 8) and crushed using a sterile pestle and mortar.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR

E.¹,

Dhlamini²,

Mbanga³

et al. 2014

IOSRJAVS

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“…Worms were washed thoroughly with PBS, pH7.2, identified morphologically according to (Periago et al 2008) and kept frozen -20°C until use.…”

Section: Parasitesmentioning

confidence: 99%

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Abdel-Rahman¹,

Mohamed

Abdel‐Rahman

et al. 2014

J Parasit Dis

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In the present study, the carbohydrate structures associated with Fasciola gigantica adult worm were identified by indirect hemagglutination inhibition test. Glucose was found to be the main monosaccharide associated with the fluke. According to indirect hemagglutination inhibition results, purification of glycoprotein fractions from worm crude extract was carried out by affinity chromatography immobilized glucose agarose gel and Con-A lectin columns. The isolated glycoprotein fractions, FI and FII, were characterized by SDS-PAGE which revealed one band in FI of 26 kDa and another one band of 19.5 kDa in FII compared with 12 bands associated with whole worm extract. Both fractions were also characterized by isoelectric focusing technique which proved that both bands were acidic in nature with pIs 6.4 and 6.5 respectively. The comparative diagnostic evaluation of the two isolated glycoprotein fractions and crude extract of experimental fasciolosis in rabbits by ELISA revealed that FII was more potent in the diagnosis during prepatent (first week post infection) and patent periods (10 weeks post infection) than FI and crude extract. Moreover, infected rabbit sera at ten weeks post infection identified both bands; 26 and 19.5 kDa in western blot analysis confirming its immunodiagnostic activities which was proved previously by ELISA. FII proved potency in diagnosis of fasciolosis in 200 buffalo serum samples of different ages and sexes using ELISA which recorded 95 % positive and 5 % negative samples. Moreover, the detailed structural analyses of the most potent fraction, F11, using mass spectrum was made and elucidated chemical structure; O-glycan [Ser-(Arg-Ser-Arg-Ser-GlucNAc) 19 -GlucNAc]. The present result introduces GlucNAc rich fraction of F .gigantica that can be used successfully in the diagnosis of acute and chronic fasciolosis.

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First phenotypic description of Fasciola hepatica/Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt

Cited by 127 publications

References 26 publications

Human Fascioliasis: A Re-emerging Disease in Upper Egypt

Human Fascioliasis: A Re-emerging Disease in Upper Egypt

Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

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