Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR

E., Chauke,; Dhlamini, Z.; Mbanga, Joshua; Dube, Simiso

doi:10.9790/2380-07211925

Cited by 5 publications

(1 citation statement)

References 22 publications

(26 reference statements)

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“…RAPD-PCR assay used to characterize F. gigantica isolates from cattle in different localities [85]. This study represented the variability of F. gigantica isolates from the same host and using RAPD markers could be applied as a low cost way of identification.…”

Section: Diagnosis Of Trematode Based On Molecular Techniquesmentioning

confidence: 99%

Advances Techniques in The diagnosis of Helminthes of Livestock

Namaky

2021

Egyptian Journal of Veterinary Sciences

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I NFECTION of the gastrointestinal tract (GIT) with helminths includes the three main groups: nematodes, cestodes and trematodes. They are detected by using conventional methods either by direct smear method or floatation /sedimentation technique which are laborious, time consuming and exhibited low sensitivity. Currently, recent progress in new diagnostic tools has opened new avenues in helminths detection. The immunological techniques which include enzyme linked immunosorbent assay (ELISA) and its modifications were appropriate for such diagnosis. They showed high sensitivity and specificity for such diagnosis. In addition, progress in molecular technique provide the potential for more reliable and efficient methods for diagnosis of hhelminthes infection. Molecular methods such as PCR (the polymerase chain reaction) , RLB (reverse line blotting), RT-PCR (real time-PCR), LAMP (loop-mediated isothermal amplification), and RFLP (restriction fragment length polymorphism) can be used as specific and sensitive tools for accurate detection of parasites DNA. PCR-based methods can be joined with RFLP or nested PCR for parasites genotypic. These combined methods can give different technique for the specific pathogen detection in stool. As well as, detection of low number of helminth parasites in stool samples by PCR is considered useful due to the higher detection sensitivity of PCR comparing to light microscopy. Recently, LAMP technique is helpful in detection of many parasitic agents and it is considered a golden tool for detection of helminths. Also, RLB method is a suitable diagnostic tool to define the characters of species in mixed infection.

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Section: Diagnosis Of Trematode Based On Molecular Techniquesmentioning

confidence: 99%

Advances Techniques in The diagnosis of Helminthes of Livestock

Namaky

2021

Egyptian Journal of Veterinary Sciences

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Morphological and molecular characterization of Fasciola hepatica and Fasciola gigantica phenotypes from co-endemic localities in Mpumalanga and KwaZulu-Natal provinces of South Africa

Haridwal

Malatji

Mukaratirwa

2021

Food and Waterborne Parasitology

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Fasciolaspecies and their vertebrate and snail intermediate hosts in East and Southern Africa: a review

2019

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A systematic review was conducted focusing on the distribution of Fasciola species and their snail intermediate hosts (IHs) in East and Southern Africa. The reviewed literature showed that both Fasciola hepatica and F. gigantica are present in East and Southern Africa, and infect a wide range of domestic and wild ruminants. Fasciola gigantica was reported in six East African and five Southern African countries, where Radix natalensis (found in low altitudes) was reported to be the main IH. Fasciola hepatica was reported in Tanzania and Ethiopia (East Africa), and in South Africa and Zimbabwe (Southern Africa), where Galba truncatula (found in high altitudes) was documented as the IH in all countries except in Zimbabwe. Both Fasciola species were documented in Tanzania, Ethiopia, Zimbabwe and South Africa. An overlap of the two was observed in areas with an intermediate altitude in Ethiopia and South Africa, where Pseudosuccinea columella was widespread and assumed to transmit both species. Pseudosuccinea columella has been reported in South Africa and Namibia, and proven to transmit F. gigantica in South Africa; its role in Namibia in the transmission of Fasciola species has not been reported. Other lymnaeid species such as R. rubiginosa were reported in South Africa, and R. auricularia in South Africa and Botswana; their role in the transmission of Fasciola species has not been proven. Future studies should aim to determine the role of P. columella in the geographical spread of the two species in East and Southern African countries.

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Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR

Cited by 5 publications

References 22 publications

Advances Techniques in The diagnosis of Helminthes of Livestock

Advances Techniques in The diagnosis of Helminthes of Livestock

Morphological and molecular characterization of Fasciola hepatica and Fasciola gigantica phenotypes from co-endemic localities in Mpumalanga and KwaZulu-Natal provinces of South Africa

Fasciolaspecies and their vertebrate and snail intermediate hosts in East and Southern Africa: a review

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