First molecular survey and novel genetic variants’ identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia

Belkahia, Hanène; Saïd, Mourad Ben; Alberti, Alberto; Abdi, Khaoula; Issaoui, Zakia; Hattab, Dorra; Gharbi, Mohamed; Messadi, Lilia

doi:10.1016/j.meegid.2015.06.017

Cited by 69 publications

(86 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is the case also in Turkey (2.3%) (Aktas et al., ), Brazil (5.4%) (Silva et al., ), Pakistan (17%) (Ashraf et al., ) and Mongolia (8.7%) (Ybañez et al., ). The difference in infection rates of A. marginale may be due to differences in tick control programmes, habitat suitability for ticks, farm management, husbandry practices, wildlife reservoir hosts and/or abiotic factors as reported by others (Belkahia et al., ; Torina et al., ).…”

Section: Discussionmentioning

confidence: 81%

“…The overall infection rate with A. marginale described in our study was 11.1%. Several studies detecting A. marginale infection in cattle have been carried out in some regions from Africa with different rates such as in Tunisia (25.4%) (Belkahia et al., ); Morocco (21.9%) (Ait Hamou et al., ); Sudan (6.1%) (Awad et al., ); Egypt (3.7%) (Younis, Hegazy, El‐Deeb, & El‐Khatib, ); Madagascar (89.7%) (Pothmann et al., ); Nigeria (75.9%) (Elelu, Ferrolho, Couto, Domingos, & Eisler, ); Kenya (32.5%) (Adjou Moumouni et al., ); Angola (38%) (Kubelová, Mazancová, & Siroky, ); and Mozambique (67%) (Machado et al., ). This is the case also in Turkey (2.3%) (Aktas et al., ), Brazil (5.4%) (Silva et al., ), Pakistan (17%) (Ashraf et al., ) and Mongolia (8.7%) (Ybañez et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…A. marginale causes mild or severe diseases characterized by fever, anaemia, weakness, constipation, jaundice, loss of appetite, dehydration, depression, laboured breathing, abortion in pregnant animals and often death in non‐treated animals (Richey & Palmer, ). With regard to the Mediterranean area, A. marginale was reported in cattle from Morocco (Ait Hamou et al., ), Tunisia (Belkahia et al., ), Italy (Zobba et al., ), Turkey (Aktas, Altay, & Dumanli, ) and Egypt (El‐Ashker et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…As this species is naturally attenuated (Rar & Golovljova, ), it is used as a live vaccine in Australia, Africa and South America (Bock, de Vos, Kingston, & Carter, ). A. centrale was identified in cattle from Tunisia (Belkahia et al., ), Italy (Ceci et al., ; Zobba et al., ), Turkey (Aktas et al., ) and Nigeria (Lorusso et al., ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Rjeibi

Ayadi

Rekik³

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

Bovine anaplasmosis could be caused by several Anaplasma species. The causative agents are transmitted by ticks and haematophagous arthropods with a high impact on both human and animal health. This study was conducted to estimate the infection rate and to characterize Anaplasma spp. in cattle from Algeria. A molecular survey was performed in Setif district (Northeast Algeria) where a total number of 180 cattle blood samples were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. PCRs revealed that the infection rates of Anaplasma spp., Anaplasma centrale, Anaplasma marginale and Anaplasma bovis were 42.2%; 39.4%; 11.1% and 4.4%, respectively. All tested animals were negative for A. phagocytophilum. Co-infection occurred in 10% (18/180) of the tested animals, and the most common co-infection pattern was an association between A. centrale and A. marginale (5.5%). Five cattle (2.7%) were co-infected by the three Anaplasma species. Holstein animals (58.1%) were more infected by A. centrale than the other breeds (p = .01). The molecular prevalence of A. centrale was significantly higher in males (54.2%) than in females (34.1%) (p = .001). A. marginale msp4 genetic analysis indicated a high sequence diversity of Algerian strains, suggesting the importation of live cattle from different origins. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. centrale revealed a low degree of genetic diversity. Our study suggests that different species of Anaplasma are simultaneously present in the Algerian cattle. To the best of our knowledge, this is the first molecular study and genetic characterization of Anaplasma spp. in Algerian cattle.

show abstract

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Rjeibi

Ayadi

Rekik³

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…The frequency of infected cattle depends geographically on the region. Thus, in the states of North Africa, for example, in Morocco and Tunisia, it is 25.4 and 29.1 % [15,16], respectively, while in Central and South Africa this index ranges from 38 to 100 % [17,18]. In the Russian Federation, according to veterinary reports, anaplasmosis is mostly recorded in the southern regions, Bryansk, Kaluga, Ryazan, Kaliningrad, Saratov, Tyumensk, Vladimir, Nizhny Novgorod, Novosibirsk and Ulyanovsk regions, Altai territory [19].…”

mentioning

confidence: 98%

CHANGES IN LEUKOCYTE AND ERYTHROCYTE BLOOD PROFILE AND PARAMETERS UNDER A COMBINED Anaplasma marginale AND BOVINE LEUKEMIA VIRUS INFECTION IN CATTLE

Kosovskii¹,

Glazko²,

Kovalchuk³

et al. 2017

S-h. biol.

View full text Add to dashboard Cite

A b s t r a c tThe global spread of infectious diseases and large-scale import of cattle genetic resources lead to necessity of developing screening methods that estimate the danger of co-infection with different pathogens and its impact on animal adaptiveness. In this regard here we analyzed the variability of erythrocyte and leukocyte characteristics in dairy Black-and-White holsteinized cattle naturally infected by Anaplasma marginale, the causative agent of bovine anaplasmosis, and bovine leukemia virus (BLV). The results showed that BLV infection of cattle did not facilitate the cross-infection with A. marginale in cattle since more than half of A. marginale-free animals were BLV-infected, and about one-third cows were characterized by leukocytosis. Except an increased number of leukocytes and lymphocytes due to retroviral infection, A. marginale-free animals were characterized only by the absence of statistically significant correlation between the counts of erythrocytes and neutrophils as compared to A. marginale-infected cows, which may point at the activation of nonspecific defense mechanisms in A. marginale-infected animals. Testing animals for BLV infection by agar gel immunodiffusion (AGID) and polymerase chain reaction (PCR) assays revealed the proviral DNA integration in one AGID-negative cow, whereas in seven out of thirty four AGID-positive cows the proviral DNA was absent. Leukocytosis (> 20½10 9 blood leukocytes per liter) was revealed only in six AGIDand PCR-positive cows. The only common feature of BLV-infected animals with moderate and severe leukocytosis was thrombocytosis, as well as disruption of correlational relationships between the number of agranulocytes and granulocytes in peripheral blood. The detected disruption of the network relationships between different leukocyte populations reflects deep changes in the immune system functioning induced by retroviral infection. We observed a deficiency of neutrophils in cows with leukocytosis, which is in consistency with the data on neutropenia in milk of BLV-infected cows with leucosis (M. Nishiike et al., 2016). Considering the absence of BLV diagnostic tests that are able to reliably exclude the false-positive or false-negative results, it seems that the most effective approach for herd sanitation may consist in a simultaneous quantification of viral load (the number of BLV RNA in peripheral blood cells) and estimation of leukocytosis severity.

show abstract

A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

et al. 2020

View full text Add to dashboard Cite

First molecular survey and novel genetic variants’ identification of Anaplasma marginale, A. centrale and A. bovis in cattle from Tunisia

Cited by 69 publications

References 72 publications

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

Molecular survey and genetic characterization ofAnaplasma centrale,A. marginaleandA. bovisin cattle from Algeria

CHANGES IN LEUKOCYTE AND ERYTHROCYTE BLOOD PROFILE AND PARAMETERS UNDER A COMBINED Anaplasma marginale AND BOVINE LEUKEMIA VIRUS INFECTION IN CATTLE

A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

Contact Info

Product

Resources

About