A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

Ashraf, Sehrish; Parveen, Asia; Awais, Mian Muhammad; Gillani, Quratulane; Aktaş, Münir; Özübek, Sezayi

doi:10.1007/s00284-020-02256-0

Cited by 25 publications

(29 citation statements)

References 21 publications

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“…Ticks are more commonly found in the areas having prolonged extreme temperature ranges, heavy rainfall, and low humidity. Such increase in temperatures and changes in rainfall patterns are highly associated with climate change [71][72][73], which may in turn increase tick infestation where it favors hatching of eggs and tick development. Furthermore, rising temperature may lead to improved survival conditions for ticks and might accelerate their reproduction and lifecycle [74].…”

Section: Discussionmentioning

confidence: 99%

An Epidemiological Survey Regarding Ticks and Tick-Borne Diseases among Livestock Owners in Punjab, Pakistan: A One Health Context

Hussain

et al. 2021

Pathogens

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Recent global changes have led to an increase in the spread of ticks and tick-borne diseases (TBDs) affecting domestic ruminants and humans, with an annual loss of US $13.9–$18.7 billion. The current study determined the perception and practices of livestock farmers regarding tick infestation. A total of 112 livestock farms were surveyed in Punjab, Pakistan, among which animals from 42 (37.5%) farms were infested with ticks. Only 28.6% (n = 32) of the dairy farmers were consulting veterinarians for ticks control, while 86.7% (n = 97) of the respondents did not consider biosecurity measures in the control of tick transmission. Most of the respondents, 71.4% (n = 80), did not consider manual tick removal from their animals (i.e., by hand, followed by physically crushing) as a risky practice for spreading zoonotic diseases. Improper disposal of bottles of acaricides in the farm drainage was also observed, putting the environment and aquatic life at risk. These wrong practices may contribute to high disease burdens and economic losses, increasing the possibility of transmission of zoonotic TBDs and pollution of the environment. Therefore, an integrated One Health approach is required for the control of TBDs through environmentally friendly approaches.

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Section: Discussionmentioning

confidence: 99%

An Epidemiological Survey Regarding Ticks and Tick-Borne Diseases among Livestock Owners in Punjab, Pakistan: A One Health Context

Hussain

et al. 2021

Pathogens

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“…Similar to age, seasons have also been highly associated with level of prevalence of babesiosis and all other TBDs (Ahmad et al, 2014; Pakistan, 2013; Siddique et al, 2020; Zahid et al, 2005). A study from Layyah conducted on prevalence of anaplasmosis (a TBD) has reported highest prevalence during autumn (18.3%), followed by that in summer (9.7%) and winter season (7.1%) (S. Ashraf et al, 2021). Summer season allied with humidity has emerged in two summer seasons in Pakistan i.e., dry summer (May, June) and wet summer (July, August).…”

Section: Discussionmentioning

confidence: 99%

“…Covering an area of 6291 km 2 , it lays at latitude of 30° N and longitude of 70° E. Its geographic characteristic is sandy land as it is entrapped between River Indus and River Chenab. It has a dry climate with scanty rainfall with a wide temperature variation of 7-41°C with an average temperature of 25.2°C (S. Ashraf et al, 2021). In summers the temperature may reach 48°C.…”

Section: Methodsmentioning

confidence: 99%

Study on Spatio-temporal Prevalence and Hematological Attributes of Bovine Babesiosis in Cattle Population of Layyah, Southern Punjab, Pakistan

Lashari

Ahmad

Rehman

2023

Preprint

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The objective of the present study was to investigate the spatiotemporal prevalence of bovine babesiosis in cattle population (n=376) of District Layyah, South Punjab, Pakistan as affected by location, age, breed, gender and seasons. Blood samples were collected aseptically and assessed for babesiosis through microscopy and PCR, and through automated analyzer for hematological attributes. Overall results of prevalence as attained through PCR in cattle population showed a significantly (P≤0.05) lower prevalence of 19.4% (n=72/376) as compared to 37.2% (n=140/276) through microscopy. None of the studied cattle from Cholistani breed were Babesia-positive. However, significantly (P≤0.05) higher prevalence was noticed for crossbred cattle (46.7%, n= 50/107) followed by that in Friesian (16.1%, n= 10/62), Jersey (7%, n= 5/71) and Sahiwal (6.9%, n= 7/101) cattle breeds. Female cattle (19.5%, n= 55/281) and age group 1 (Up to 2 years) (40%, n= 42/105) had higher prevalence of Babesia as ascertained through PCR in comparison to their counterpart groups. Significantly (P≤0.05) higher prevalence of 35.9% (n=60/167) was shown in summer as compared to that in winter season (5.7%, n= 12/209). All the positive samples produced the 490bp amplicons specific and typical for Babesia bigemina. Regarding hematology, hemoglobin concentration, erythrocytic count, hematocrit and mean corpuscular volume were significantly (P≤0.05) lower in babesia-positive cattle as compared to healthy ones. In a nutshell, indigenous cattle breeds are tick-resistant hardy breeds and do not show severe signs of babesiosis as compared to exotic and crossbred cattle. Furthermore, Southern Punjab area of Pakistan has a different spatiotemporal distribution of babesiosis with bigemina being predominant.

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“…In developing countries, more than 50% of production losses are due to TTBPs, mainly Anaplasma and Theileria (Ashraf et al, 2021). Various studies have been conducted to date for the estimation of tick-borne parasitic diseases through conventional methods but due to low sensitivity and specificity, none of them was declared as a gold standard for true estimation of the prevalence of TBPs in the animal population.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Development of Snakehead (Channa argus) Industry in Shandong, China and Comparison with the Substitution of Forage Fish with Compound Feed

Li¹

2023

PJZ

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A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan)

Cited by 25 publications

References 21 publications

An Epidemiological Survey Regarding Ticks and Tick-Borne Diseases among Livestock Owners in Punjab, Pakistan: A One Health Context

An Epidemiological Survey Regarding Ticks and Tick-Borne Diseases among Livestock Owners in Punjab, Pakistan: A One Health Context

Study on Spatio-temporal Prevalence and Hematological Attributes of Bovine Babesiosis in Cattle Population of Layyah, Southern Punjab, Pakistan

Analysis of the Development of Snakehead (Channa argus) Industry in Shandong, China and Comparison with the Substitution of Forage Fish with Compound Feed

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