First Isolation of Vibrio tapetis, the Etiological Agent of Brown Ring Disease (BRD), in Manila Clam Ruditapes philippinarum in Japan

Matsuyama, Tomomasa; Sakai, Takamitsu; Kiryu, Ikunari; Yuasa, Kei; Yasunobu, Hideki; Kawamura, Yoshihiro; Sano, Motohiko

doi:10.3147/jsfp.45.77

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…The Norwegian S2-2 strain was kindly offered by S Mortensen (2009, unpublished data) from the Institute of Marine Research at Bergen in Norway. Finally, the FPC 1121 strain was graciously provided by T Matsuyama ( Matsuyama et al, 2010 ). The other strains were available from the LEMAR collection.…”

Section: Methodsmentioning

confidence: 99%

Development of a Taqman real-time PCR assay for rapid detection and quantification ofVibrio tapetisin extrapallial fluids of clams

Bidault

Richard

Bris

et al. 2015

PeerJ

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The Gram-negative bacterium Vibrio tapetis is known as the causative agent of Brown Ring Disease (BRD) in the Manila clam Venerupis (=Ruditapes) philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification of V. tapetis strains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600T V. tapetis strain. Quantification curves of V. tapetis strain seeded in filtered seawater (FSW) or extrapallial fluids (EF) samples were equivalent showing reliable qPCR efficacies. With this protocol, we were able to specifically detect V. tapetis strains down to 1.125 101 bacteria per mL of EF or FSW, taking into account the dilution factor used for appropriate template DNA preparation. This qPCR assay allowed us to monitor V. tapetis load both experimentally or naturally infected Manila clams. This technique will be particularly useful for monitoring the kinetics of massive infections by V. tapetis and for designing appropriate control measures for aquaculture purposes.

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Section: Methodsmentioning

confidence: 99%

Development of a Taqman real-time PCR assay for rapid detection and quantification ofVibrio tapetisin extrapallial fluids of clams

Bidault

Richard

Bris

et al. 2015

PeerJ

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“…The two subspecies are easily distinguishable by their capacity to produce acid from mannitol and arabinose and for their metabolization of citrate (Balboa and Romalde, 2013). Many strains have been isolated from molluscs and fish since the initial description of this disease (Castro et al, 1992;Maes and Paillard, 1992 ;Jensen et al, 2003;Reid et al, 2003;Matsuyama et al, 2010;Lopez et al, 2011). Interestingly the highest virulence recorded is still attributed to the first strain, CECT4600, isolated in Landéda in 1990 and contains a large conjugative plasmid pVT1 (Erauso et al, 2011).…”

Section: Tapetismentioning

confidence: 99%

Bacterial diseases in marine bivalves

Travers

Miller

Roque

et al. 2015

Journal of Invertebrate Pathology

142

121

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Bivalve aquaculture is seriously affected by many bacterial pathogens that cause high losses in hatcheries as well as in natural beds. A number of Vibrio species, but also members of the genera Nocardia and Roseovarius, are considered important pathogens in aquaculture. The present work provides an updated overview of main diseases and implicated bacterial species affecting bivalves. This review focuses on aetiological agents, their diversity and virulence factors, the diagnostic methods available as well as information on the dynamics of the host-parasite relationship.

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“…FPC1121 was isolated from cultivated Manila clam in Japan (Table 1, Matsuyama et al, 2010). The original report clearly described the brown deposit and mass mortalities of Manila clam due to this strain, which was confirmed by in vivo virulence assays (Matsuyama et al, 2010). This clustering of FPC1121 is an interesting result; detailed genetic analysis would be interesting to explore the phylogenetic position of this strain and the linked genetic differences.…”

Section: Maldi-tof Ms Analysis Reveals 3 Clusters Of V Tapetis Isolatesmentioning

confidence: 76%

“…FPC1121 is the only clam pathogenic strain that was clustered together with fish isolates, based on the MALDI-TOF MS profiling. FPC1121 was isolated from cultivated Manila clam in Japan (Table 1, Matsuyama et al, 2010). The original report clearly described the brown deposit and mass mortalities of Manila clam due to this strain, which was confirmed by in vivo virulence assays (Matsuyama et al, 2010).…”

Section: Maldi-tof Ms Analysis Reveals 3 Clusters Of V Tapetis Isolatesmentioning

confidence: 81%

MALDI-TOF MS as a promising tool to assess potential virulence of Vibrio tapetis isolates

et al. 2021

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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First Isolation of Vibrio tapetis, the Etiological Agent of Brown Ring Disease (BRD), in Manila Clam Ruditapes philippinarum in Japan

Cited by 9 publications

References 12 publications

Development of a Taqman real-time PCR assay for rapid detection and quantification ofVibrio tapetisin extrapallial fluids of clams

Development of a Taqman real-time PCR assay for rapid detection and quantification ofVibrio tapetisin extrapallial fluids of clams

Bacterial diseases in marine bivalves

MALDI-TOF MS as a promising tool to assess potential virulence of Vibrio tapetis isolates

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