MALDI-TOF MS as a promising tool to assess potential virulence of Vibrio tapetis isolates

Rahmani, Alexandra; Vercauteren, Maaike; Vranckx, Katleen; Boyen, Filip; Bidault, Adeline; Pichereau, Vianney; Decostere, Annemie; Paillard, Christine; Chiers, Koen

doi:10.1016/j.aquaculture.2020.735729

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…The time‐to‐identification can be decreased when compared to other bacteriologic techniques such as biochemical profiling or immunofluorescent assays, and this is particularly true for slow‐growing organisms like R. salmoninarum . Its application and feasibility in the identification of bacterial species of freshwater and seawater finfish and crustacean species have been extensively reported (Bronzato et al, 2018; Jung‐Schroers et al, 2018; 2019; Moussa et al, 2021; Nhu et al, 2016; Ojasanya et al, 2022; Pečur Kazazić et al, 2019; Rahmani et al, 2021; Yolanda et al, 2019), including for the fastidious salmonid pathogens R. salmoninarum (Jansson et al, 2020), Flavobacterium species (Fernández‐Álvarez et al, 2018; Jansson et al, 2020), Piscirickettsia salmonis (Olate et al, 2016) and Tenacibaculum maritimum (Fernández‐Álvarez et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Renibacterium salmoninarum infection in harvested Atlantic salmon (Salmo salar L.) on the east coast of Canada: Clinical findings, sample collection methods and laboratory diagnostic tests

Jia

Burnley

Gardner

et al. 2023

Journal of Fish Diseases

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Chronic subclinical infection with the aetiological agent of bacterial kidney disease (BKD), Renibacterium salmoninarum, presents challenges for the clinical management of disease in farmed salmonids and for prevalence estimation. Harvested salmon sampled at processing plants provide the opportunity to describe subclinical outcomes of BKD using gross necropsy observations and diagnostic test results in farmed Atlantic salmon (Salmo salar L.) populations that are apparently healthy (i.e. alive at harvest) but naturally exposed to R. salmoninarum infection. Sampling of farmed

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Section: Discussionmentioning

confidence: 99%

Diagnosis of Renibacterium salmoninarum infection in harvested Atlantic salmon (Salmo salar L.) on the east coast of Canada: Clinical findings, sample collection methods and laboratory diagnostic tests

Jia

Burnley

Gardner

et al. 2023

Journal of Fish Diseases

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“…Three sub-species have been described for V. aestuarianus aestuarianus , V. aestuarianus francensis associated with cupped oyster diseases, and V. aestuarianus cardii , associated with cockle diseases (Garcia et al 2021 ). Further analysis could be applied to sub-type species or to discriminate between pathogenic and non-pathogenic strains, e.g., the “MALDI-TOF-MS Multi Peak Shift Typing approach,” highlighting a link between a peak shift in the mass spectra and the associated polymorphism in the genomic sequence (Bridel et al 2020 ; Rahmani et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks

Moussa

Cauvin

Piouffle³

et al. 2021

Appl Microbiol Biotechnol

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In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was constructed by the merger of raw spectra obtained from three different media and generated by three collaborating laboratories to increase the diversity of the conditions and thus obtain a good technique robustness. Perfect discrimination was obtained with all of the MSP created for the Vibrio species and even for very closely related species as V. europaeus and V. bivalvicida. The new EnviBase library was validated through a blind test on 100 Vibrio strains performed by our three collaborators who used the direct transfer and protein extraction methods. The majority of the Vibrio strains were successfully identified with the newly created EnviBase by the three laboratories for both protocol methods. This study documents the first development of a freely accessible database exclusively devoted to Vibrio found in marine environments, taking into account the high diversity of this genus. Key points • Development of a MALDI-TOF MS database to quickly affiliate Vibrio species. • Increase of the reactivity when faced with Vibrio associated with mollusk diseases. • Validation of MALDI-TOF MS as routine diagnostic tool.

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“…Low et al (2014) reported the identification of V. alginolyticus from Epinephelus fuscoguttatus (brown-marbled grouper) using MALDI TOF. Additionally, Rahmani et al (2021) also came to the conclusion that V. tapetis isolated from Manila clams, were found as pathogenic based on the protein profiles which demonstrated the presence of a virulence gene. V. parahaemolyticus, the causative agent of However, there are some limitations to take into account.…”

Section: Vibrio Spp and Other Fish Pathogensmentioning

confidence: 97%

Application of the MALTI-TOF MS Method for Identification of Vibrio spp. in Aquaculture

Gökdağ,

Çağatay

2024

Marine Science and Technology Bulletin

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Aquaculture is developing rapidly and plays an important role in providing animal protein to the world’s growing population. However, increasing mortality from bacterial disease outbreaks in important species poses a challenge to production progress in this sector. In order to reduce the impact of these diseases, rapid and accurate pathogen identification is essential for disease management, early detection and the continued health of aquaculture. The aim of this review is to summarise studies on the identification and diagnosis of Vibrio pathogens in aquatic organisms by MALTI-TOF MS (Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry), a rapid identification method based on protein profiling of bacteria. The profiles of bacterial protein obtained are compared with a global microbial protein library for identification. This study demonstrates the potential of using MALDI-TOF MS for the detection of Vibrio pathogens in aquaculture in studies published between 2015 and 2024. While purchasing a time-of-flight mass spectrometer is expensive when compared to conventional and molecular identification methods. It also appears to be much more efficient in terms of time spent on identification. MALDI-TOF MS has been shown to be simple to use in fish identification laboratories.

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MALDI-TOF MS as a promising tool to assess potential virulence of Vibrio tapetis isolates

Cited by 6 publications

References 30 publications

Diagnosis of Renibacterium salmoninarum infection in harvested Atlantic salmon (Salmo salar L.) on the east coast of Canada: Clinical findings, sample collection methods and laboratory diagnostic tests

Diagnosis of Renibacterium salmoninarum infection in harvested Atlantic salmon (Salmo salar L.) on the east coast of Canada: Clinical findings, sample collection methods and laboratory diagnostic tests

A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks

Application of the MALTI-TOF MS Method for Identification of Vibrio spp. in Aquaculture

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