First identification of Sarcocystis hominis in Iranian traditional hamburger

Ahmadi, Mehdi; Hajimohammadi, Bahador; Eslami, Gilda; Oryan, Ahmad; Ardakani, S. A. Yasini; Zohourtabar, A; Zare, S.

doi:10.1007/s12639-014-0425-7

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…The first case identification of S. hominis in hamburgers in Iran was reported by Moghaddam Ahmadi et al (2015). However, a pervious study on industrial and traditional raw hamburgers in Yazd, Iran showed that a high prevalence of infection to S. hominis (Hajimohammadi et al 2014a).…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Sarcocystis species in raw hamburgers using PCR–RFLP method in Kashan, central Iran

Hooshyar

Abbaszadeh

Sharafati-Chaleshtori

et al. 2017

J Parasit Dis

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The prevalence of bovine is high in the most regions of the world. It can be a human health problem due to consumption of raw or under cooked hamburgers or other bovine meat products. This study was carried out to investigate the prevalence and species identification of among of hamburgers, using PCR-RFLP methods in Kashan, central Iran. Overall 200 raw industrial hamburgers samples with at least 60% meat were randomly collected from nine different brands in Kashan, central Iran. The genomic DNA was extracted and a PCR-RFLP method was used to amplify an approximately 900 bp fragment at the 18S rRNA(SSU) gene, restriction enzyme was used for species identification. The results showed that 58 (29%) of 200 tested hamburger samples were infected to spp. The prevalence rate was 31.25 and 26.9% in the hamburgers with 90 and 60-75% meat, respectively. According to PCR-RFLP analysis, 43 (74.1%) of the 58 isolates were , 12 (20.7%) showed co-infection to and , 2 (3.5%) was mixed infected to and , 1 (1.7%) showed the pattern of mix infection to three species. This study revealed one-third of industrial hamburger were infected to or mixed infection of with other bovine sarcocytosis. To prevent cattle infection, the possible ingestion of the disposal sporocyst stage from dogs must be eliminated. Although in this study, the prevalence of was low and cannot be considered as a major zoonosis, it should be recommended avoiding eating under cooked hamburger and other bovine meat products to prevent human infection.

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Section: Discussionmentioning

confidence: 99%

Molecular identification of Sarcocystis species in raw hamburgers using PCR–RFLP method in Kashan, central Iran

Hooshyar

Abbaszadeh

Sharafati-Chaleshtori

et al. 2017

J Parasit Dis

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“…In another study, we reported the prevalence of 57.5% for S. hominis in cattle meat in Yazd, Iran (Hajimohammadi et al, 2014d). Also, Hajimohammadi et al (2014a) and Moghaddam-Ahmadi et al, (2015) reported the first S. hominis and S. hirsuta from beef hamburger. However, there is still no data on the presence of S. sinensis in beef samples produced in this country.…”

Section: Introductionmentioning

confidence: 98%

Sarcocystis sinensis in slaughtered cattle from Central of Iran

Hajimohammadi

Eslami

Manafi

et al. 2022

J Hellenic Vet Med Soc

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Sarcocystis is a food borne parasite as an intracellular parasite with two-host life cycle. Humans serve as either intermediate or definitive hosts among different species. Sarcocystosis is a very important disease because of its widespread prevalence with an impact on production and animal health. We aimed to assess S. sinensis in cattle from the desert area of Iran, using conventional PCR, targeting 18S rRNA. Also, the phylogenic analysis was done to mention isolate and the related ones in Genbank.From the 108 beef samples of heart, esophagus, intercostal muscle, diaphragm, and tongue harboring Sarcocystis spp. stored in the BioBank of the Research Center for Health and Food Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, DNA extraction was done. Amplification was done using the primer pair of SAR-F: 5'-TGGCTAATACATGCGCAAATA-3' and SAR-R: 5'-AACTTGAATGATCTATCGCCA-3'For the target of 18S rRNA gene. Sequencing was done. The interesting sequence was edited and then analyzed with BLAST. Then, multiple alignments were conducted using T-coffee. The mentioned sequence was submitted to GenBank in NCBI. Evolutionary analyses were conducted in MEGA7. In order to statistical analysis of the presence of Sarcocystis sinensis significantly with sex, age, and location, the Fisher’s Exact test was done using SPSS 16.0 (SPSS Inc. Chicago, USA). The p-value < 0.05 was considered significant.Out of 108 samples, five isolates were identified as S. sinensis. The distribution of S. sinensis was related to age (p<0.05) but had no significant difference with gender (p>0.05). Phylogenic analysis showed a similarity of S. sinensis with S. hominis. From a food safety viewpoint, this study employed a molecular approach to identify S. sinensisin in cattle meat. To the best of our knowledge, this is the first report of S. sinensis in the desert area from Iran.

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“…In the future we think that it would be appropriate to carry out more detailed studies covering different gene regions for both S. hominis and S. bovini. In addition, in our bordering neighbour Iran, S. hominis was detected in a molecular study on raw hamburgers (24).…”

Section: Discussionmentioning

confidence: 92%

“…In particular, the 18S rRNA gene region is more widely used for the identification of Sarcocystis spp. than the other gene regions (4,16,18,23,24,27,33,42). However; it has been reported that S. hominis, S. bovifelis, S. bovini, and S. rommeli species cannot be distinguished by the 18S rRNA gene region, and the term 'S.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey

Oğuz

Değer

Koşal

2021

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.

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First identification of Sarcocystis hominis in Iranian traditional hamburger

Cited by 10 publications

References 16 publications

Molecular identification of Sarcocystis species in raw hamburgers using PCR–RFLP method in Kashan, central Iran

Molecular identification of Sarcocystis species in raw hamburgers using PCR–RFLP method in Kashan, central Iran

Sarcocystis sinensis in slaughtered cattle from Central of Iran

Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey

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