First <i>Report of Cucurbit aphid-borne yellows virus</i> Affecting Chickpea (<i>Cicer arietinum</i> L.) in Sudan

Kumari, Safaa G.; Moukahel, Abdul Rahman; Hamed, A. A.; Sharman, Murray

doi:10.1094/pdis-02-18-0347-pdn

Cited by 9 publications

(6 citation statements)

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“…The present study is the first to report CLRDV and PABYV from crops in Sudan, and is the first report of PeVYV isolated from chickpea in this country. Kumari et al (2018) made the first report of CABYV in Sudan, and the present report has greatly extended the understanding of the diversity, geographical range and incidence of CABYV in Sudan. In addition, this study is the first reliable molecular characterization for these four Polerovirus species identified from chickpea samples collected in Sudan.…”

Section: Discussionmentioning

confidence: 57%

“…Although results of serological tests confirmed the growing importance and challenge caused by luteovirids in legume crops in the WANA region, there have been many indications that the use of serological techniques Kumari et al (2018).…”

Section: Discussionmentioning

confidence: 99%

“…Field surveys were conducted in the major chickpea production areas in Sudan, including areas of the Gezira Scheme (middle, north and south) and River Nile State (Hudeiba Agriculture Research Station, Berber and Shendi). The 204 chickpea samples collected by Kumari et al (2018) in February 2013 and March 2015 were included in the present study, to investigate luteovirid diversity in addition to the CABYV already reported. A further 211 chickpea samples were collected in February 2018 when the crops were at the flowering/pod setting stage.…”

Section: Field Surveys and Serological Testsmentioning

confidence: 99%

“…These are, Faba bean necrotic yellows virus (FBNYV, Nanovirus, Nanoviridae), Chickpea chlorotic dwarf virus (CpCDV, Mastrevirus, Geminiviridae), CpCSV and BWYV (Abra-ham et al, 2009;Makkouk et al, 2003b;Makkouk, 2020). Cucurbit aphid-borne yellows virus (CABYV) has also been reported by Kumari et al (2018) to infect chickpea in Sudan and cause stunting, yellowing and necrosis. However, that study suggested the presence of other luteovirids in survey samples, but the identity of these was not reported.…”

Section: Introductionmentioning

confidence: 99%

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Distribution and identification of luteovirids affecting chickpea in Sudan

Moukahel

Kumari

Hamed

et al. 2021

Phytopathol. Mediterr.

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In Sudan yellowing viruses are key production constraints in pulse crops. Field surveys were carried out to identify luteovirids affecting chickpea crops in the major production regions (Gezira Scheme and River Nile State). A total of 415 chickpea plant samples with yellowing and stunting symptoms were collected during the 2013, 2015 and 2018 growing seasons. Serological results (Tissue-blot immunoassays) showed that Luteoviridae and Chickpea chlorotic dwarf virus (CpCDV, genus Mastrevirus, family Geminiviridae) were the most common viruses, with rare infections with Faba bean necrotic yellows virus (FBNYV, genus Nanovirus, family Nanoviridae). Some samples reacted only with a broad-spectrum luteovirid monoclonal antibody (5G4-MAb), and others showed cross reactions between the specific monoclonal antibodies, suggesting the occurrence of new luteovirid variants. Serological results were confirmed by amplification with reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the partial coat protein gene. Molecular analyses provided a basic, sufficient and reliable characterization for four viruses affecting chickpea that belong to Polerovirus (family Luteoviridae). These were Cucurbit aphid-borne yellows virus (CABYV), Pepper vein yellows virus (PeVYV), Pepo aphid-borne yellows virus (PABYV) and Cotton leafroll dwarf virus (CLRDV), that shared high similarity with the type sequences. Phylogenetic analyses also revealed high similarity to luteovirid species. This study has established reliable, rapid and sensitive molecular tools for the detection of luteovirid species.

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Field Surveys and Serological Testsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution and identification of luteovirids affecting chickpea in Sudan

Moukahel

Kumari

Hamed

et al. 2021

Phytopathol. Mediterr.

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“…CLRDV was initially identified as an infestation of cotton, causing stunting, leaf curling, dense green leaves, yellowing of veins, brittle leaves, reduced flowers and boll size, and plant sterility in some cases, which can lead to yield losses of up to 80% in some susceptible varieties [ 5 ]. CLRDV virus may also infect chickpea, causing symptoms similar to those of cotton (reddening of leaves, shortening of internodes, and stunting and browning of the bast) [ 21 , 24 , 32 ]. Similar symptoms of chickpeas can also be seen in CABYV infestation.…”

Section: Virus Characteristics Of Chickpeamentioning

confidence: 99%

Identification of New Chickpea Virus and Control of Chickpea Virus Disease

Cun

2022

Evidence-Based Complementary and Alternative Medicine

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Objective. The objective of the study was to discuss the classification, virus characteristics, detection methods, and control measures of chickpea virus, with an aim to provide a theoretical basis for identification of new chickpea virus and control of chickpea virus disease. Methods. The domestic and foreign studies were reviewed, and the virus coat protein or nucleic acid sequence was identified by immunological and molecular diagnostic techniques. Results. There were 14 main types of chickpea viruses attacking, and seven Luteoviridae viruses were reported, namely, chickpea chlorotic stunt virus (CpCSV), bean leafroll virus (BLRV), beet western yellows virus (BWYV), soybean dwarf virus (SbDV), cotton leafroll dwarf virus (CLRDV), cucurbit aphid-borne yellows virus (CABYV), and phasey bean mild yellows virus (PhBMYV). The family Geminiviridae includes chickpea chlorotic dwarf virus (CpCDV), chickpea chlorosis virus (CpCV), chickpea redleaf virus (CpRLV), chickpea yellows virus (CpYV), and mastrevirus. The family Nanoviridae is dominated by the faba bean necrotic yellows virus (FBNYV). The family Bromoviridae includes cucumber mosaic virus (CMV) and alfalfa mosaic virus (AMV). Conclusion. At present, there are mainly 12 types of viruses infecting chickpeas, which are transmitted by leafhoppers or aphids and are associated with symptoms such as yellowing, chlorosis, and stunted pod development, resulting in serious yield loss. Correct use of various molecular diagnostic tools to detect and identify chickpea virus can accurately assess chickpea virus infection and provide a basis for the prevention and treatment of chickpea virus disease.

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Breeding and genetics of resistance to major diseases in Cucurbita—A review

Sabharwal,

Thakur,

Shrestha

et al. 2024

Crop Science

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Cucurbita crops, which include market types of pumpkin and squash, have unparalleled fruit variation and equally important economic value worldwide. Pumpkin and squash have versatile uses but are most popular in culinary, ornamental, snacking, and seed oil industries. The production of Cucurbita crops is hindered by diseases caused by fungal, oomycetes, and viral pathogens. Host resistance is an important component of integrated disease management for Cucurbita crops and is a major goal for plant breeders. This review addresses the major diseases of Cucurbita, including powdery mildew, downy mildew, Phytophthora rot, and aphid and whitefly transmitted viruses, with an emphasis on germplasm exploitation for the development of resistant cultivars. Resistance to powdery mildew derived from Cucurbita okeechobeensis subsp. martinezii (designated PM‐0) has been extensively deployed in commercial cultivars and was recently mapped on chromosomes 3 and 10 of Cucurbita moschata and Cucurbita pepo, respectively. Resistance to Phytophthora crown rot is present across several Cucurbita species, including Cucurbita lundelliana, C. okeechobeenesis subsp. okeechobeenesis, C. moschata, and C. pepo. Mapping studies have identified significant loci associated with Phytophthora crown rot resistance on chromosomes 4, 11, and 20 of C. moschata and chromosomes 4, 5, 8, 12, 13, 16, and 19 of C. pepo. Sources of resistance to aphid‐transmitted viruses exist in C. moschata, Cucurbita ficifolia, Cucurbita ecuadorensis, Cucurbita martinezii, C. ecuadorensis, Cucurbita maxima, and Cucurbita foetidissima. The availability of DNA markers linked to resistance against zucchini yellow mosaic virus and papaya ringspot virus in C. moschata and C. pepo has facilitated marker‐assisted selection (MAS) in breeding programs. On the other hand, sources of resistance to tomato leaf curl New Delhi virus (ToLCNDV), a major whitefly‐transmitted virus in Cucurbita, have been identified in C. moschata, C. lundelliana, and C. okeechobeensis. A major locus conferring resistance to ToLCNDV in C. moschata was recently mapped on chromosome 8 enabling the application of MAS with a prediction accuracy of 94.3%. Overall, the continued discovery and application of genomic tools for resistance breeding in Cucurbita will accelerate the rate of genetic gain while reducing costs associated with phenotyping.

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First Report of Cucurbit aphid-borne yellows virus Affecting Chickpea (Cicer arietinum L.) in Sudan

Cited by 9 publications

References 3 publications

Distribution and identification of luteovirids affecting chickpea in Sudan

Distribution and identification of luteovirids affecting chickpea in Sudan

Identification of New Chickpea Virus and Control of Chickpea Virus Disease

Breeding and genetics of resistance to major diseases in Cucurbita—A review

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