First histological observations on the incorporation of a novel calcium phosphate bone substitute material in human cancellous bone

Sarkar, Michael R.; Wachter, N. J.; Kinzl, L.

doi:10.1002/1097-4636(2001)58:3<329::aid-jbm1025>3.3.co;2-0

Cited by 30 publications

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“…39 Although the untreated defects still maintained, as expected, the characteristics of nonhealed defects, a comparison of the healing rate of the current internal control defects (group 4: right side treated with FDBA þ PRP þ BMSC and left side left untreated) was made with that of external control defects, belonging to studies, where no proteins, cells, or growth factors were used, 34,38 in order to confirm that crossed influence did not occur. 9,29,40,41 No significant differences were actually highlighted (data not shown).…”

Section: Discussionmentioning

confidence: 86%

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In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

156

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The repair of confined trabecular bone defects in rabbits treated by autologous bone marrow stromal cells (BMSC), platelet-rich plasma (PRP), freeze-dried bone allografts (FDBA) alone and in combination (BMSC þ PRP; FDBA þ BMSC; FDBA þ PRP; FDBA þ PRP þ BMSC) was compared. A critical size defect was created in the distal part of the femurs of 48 adult rabbits. Histology and histomorphometry were used in the evaluation of healing at 2, 4, and 12 weeks after surgery. The healing rate (%) was calculated by measuring the residual bone defect area. Architecture of the newly formed bone was compared with that of bone at the same distal femur area of healthy rabbits. The defect healing rate was higher in PRP þ BMSC, FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC treatments, while lower values were achieved with PRP treatment at all experimental times. The highest bone-healing rate at 2 weeks was achieved with FDBA þ PRP þ BMSC treatment, which resulted significantly different from PRP (p < 0.05) and BMSC (p < 0.05) treatments. At 4 weeks, the bone-healing rate increased except for PRP treatment. Finally, the bone-healing rate of FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC was significantly higher than that of PRP at 12 weeks (p < 0.05). At 12 weeks, significant differences still existed between PRP, BMSC, and FDBA groups and normal bone (p < 0.05). These results showed that the combination of FDBA, BMSC and PRP permitted an acceleration in bone healing and bone remodeling processes. ß

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Section: Discussionmentioning

confidence: 86%

“…40,41 This could be the cause of the complete readsorption of FDBA in the healing defects at 12 weeks.…”

Section: Discussionmentioning

confidence: 99%

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

156

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“…In previous studies, this bone growth-inducing property was mimicked by coating carriers with amorphous metastable form of calcium phosphate particles. The histological studies showed that amorphous form has high resorbability and bioactivity because of the higher bonding affinity (Overgaard et al 1999;Sarkar et al 2001). In light of these advantages, in this study, we aimed to synthesize amorphous calcium phosphate nanoparticles by the FSP method with calcium acetate hydrate as a Ca source and propionic acid as the solvent.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

et al. 2015

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The present study evaluates the synthesis of biocompatible osteoconductive and osteoinductive nano calcium phosphate (CaP) particles by industrially applied, aerosol-derived flame spray pyrolysis method for biomedical field. Calcium phosphate nanoparticles were produced in a range of calciumto-phosphorus ratio, (1.20-2.19) in order to analyze the morphology and crystallinity changes, and to test the bioactivity of particles. The characterization results confirmed that nanometer-sized, spherical calcium phosphate particles were produced. The average primary particle size was determined as 23 nm by counting more than 500 particles in TEM pictures. XRD patterns, HRTEM, SAED, and SEM analyses revealed the amorphous nature of the asprepared nano calcium phosphate particles at low Ca/P ratios. Increases in the specific surface area and crystallinity were observed with the increasing Ca/P ratio. TGA-DTA analysis showed that the thermally stable crystal phases formed after 700°C. Cell culture studies were conducted with urine-derived stem cells that possess the characteristics of mesenchymal stem cells. Synthesized amorphous nanoparticles did not have cytotoxic effect at 5-50 lg/ml concentration range. Cells treated with the as-prepared nanoparticles had higher alkaline phosphatase (ALP) enzyme activity than control cells, indicating osteogenic differentiation of cells. A slight decrease in ALP activity of cells treated with two highest Ca:P ratios at 50 lg/ml concentration was observed at day 7. The findings suggest that calcium phosphate nanoparticles Guest Editors: Mustafa Culha, Rawil F. Fakhrullin, Ratnesh Lal This article is part of the topical collection on Nanobiotechnology Electronic supplementary material The online version of this article

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“…This is done to avoid relative shortening of the patellar ligament as well as an increase of the posterior slope of the tibia. We used a porous beta-tricalcium phosphate (β-TCP) wedge because it is resorbable and osteoinductive [10][11][12]. The wedge (Ceraver implant; Ceraver TM , France) was held in place by the compressive force in the opened osteotomy.…”

Section: Introduction Of the Wedge At The Osteotomy Sitementioning

confidence: 99%

Opening wedge tibial osteotomy for large varus deformity with CeraverTM resorbable beta tricalcium phosphate wedges

Hernigou

Roussignol

Flouzat-Lachaniette

et al. 2009

International Orthopaedics (SICOT)

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The results in 53 knees that had been treated by proximal tibial opening-wedge osteotomy for large varus deformity and osteoarthritis of the medial compartment were evaluated after a mean length of follow-up of ten years (range, 8-12 years). We used a porous betatricalcium phosphate (β-TCP) wedge because it is resorbable and osteoinductive. All osteotomies were completely consolidated and complete osseointegration of the remnant of the β-TCP wedge took place. However, after a mean maximum follow-up of ten years none of the cases showed complete resorption. After ten years, 40 (81%) of the 53 knees had an excellent or good result, and in 13 knees there was recurrent pain for which six had an arthroplasty. Although the results deteriorated with time, time was not the only determinant of the result. Alignment, measured as the hip-knee-ankle angle on radiographs of the whole limb that were made with the patient bearing weight, was also a determinant of long-term results. The best results were obtained in the knees that had a hip-knee-ankle angle of 183-186 degrees. In these knees, there was no pain and no progression of the arthrosis in either the medial or the lateral tibiofemoral compartment. Of the three knees that had an angle of more than 186 degrees, all five had progressive degenerative changes in the lateral compartment. In the undercorrected knees (an angle of less than 183 degrees), the results were less satisfactory, and there was a tendency toward recurrence of the varus deformity and progression of the arthritis of the medial compartment. However, when the correction was insufficient the deterioration was slow. Therefore, proximal tibial osteotomy is a very suitable operation even for patients who have gonarthrosis of the medial compartment and a large varus deformity. Although, a rigidly standardised and precise operative technique is required as well as accurate radiographic measurements of the mechanical axis of the limb because exact postoperative alignment is the prerequisite for the longest possible period of relief of symptoms after osteotomy, and this exact alignment is difficult to obtain for patients with large varus deformity.

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First histological observations on the incorporation of a novel calcium phosphate bone substitute material in human cancellous bone

Cited by 30 publications

References 0 publications

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

Opening wedge tibial osteotomy for large varus deformity with CeraverTM resorbable beta tricalcium phosphate wedges

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