First Genome Sequence of Foot-and-Mouth Disease Virus Serotype O Sublineage Ind2001e from Southern Vietnam

Abstract

We report the polyprotein coding sequence of the newly defined Ind2001e sublineage of foot-and-mouth disease virus (FMDV) serotype O, isolated from a bovine epithelial tissue sample collected in 2017 in Kon Tum Province, Vietnam. This discovery updates FMDV diversity in Vietnam, has implications for FMDV epidemiology, and influences future vaccine selections.

Genome Sequences of 18 Foot-and-Mouth Disease Virus Outbreak Strains of Serotype O Sublineage Ind2001d from India, 2013 to 2014

“…FMDV was confirmed by the detection of viral RNA in tissue homogenate using FMDV-specific real-time reverse transcription-PCR (rRT-PCR) or by virus isolation (VI) on BHK21 or LFBK-α v β 6 cells followed by the detection of viral RNA in VI supernatant by rRT-PCR (9, 10). Total cell supernatant or tissue homogenate RNA was subjected to viral deep sequencing, as previously described (11). Briefly, RNA underwent first-strand synthesis using the SuperScript II first-strand synthesis system (Invitrogen) coupled with random primers and two FMDV-specific primers, one poly(T) primer [targeting the 3′ poly(A) region] and an FMDV universal reverse primer (12), which binds within the 2A coding region.…”

Adenovirus-Vectored Capsid Proteins of the Serotype A Foot-and-Mouth Disease Virus Protect Guinea Pigs Against Challenge

“…The individual mature proteins VP1, VP3, and VP0 spontaneously form the 5S protomer, five of which assemble into a 12S pentamer. Twelve pentamers then assemble into the 75S viral capsid (Senthilkumaran et al, 2017;Palinski and Bertram, 2019). The viral capsid has been reported to have very similar antigen specificity as the complete FMDV 146S antigen (Rodriguez and Grubman, 2009;Kushnir et al, 2012;Scotti and Rybicki, 2013).…”

Foot-and-Mouth Disease Virus Serotype O/CATHAY Genome Sequences from Five Outbreaks in Vietnam, 2017 to 2019

“…At the FADRU, FMDV was confirmed by real-time reverse transcription-PCR (rRT-PCR) using FMDV-specific primers and by virus isolation on LFBK-␣v␤6 cells followed by rRT-PCR (9, 10). Total cell supernatant or tissue homogenate RNA was subjected to deep sequencing, as described previously (11,12). Briefly, RNA was extracted from supernatant using the MagMAX total RNA isolation kit, and DNA was depleted using the DNA-free DNase kit (Ambion).…”

“…Briefly, RNA was extracted from supernatant using the MagMAX total RNA isolation kit, and DNA was depleted using the DNA-free DNase kit (Ambion). Treated RNA underwent first-strand synthesis using the SuperScript first-strand synthesis system (Invitrogen) with random hexameric primers, a poly(T) primer targeting the 3= untranslated region (UTR), and one FMDVspecific reverse primer (GCCCRGGGTTGGACTC), which improves sensitivity and ensures specificity (11,13). Double-stranded cDNA was generated using the NEBNext Ultra II nondirectional RNA second-strand synthesis module (New England Biolabs).…”