First genome sequence of a blaKPC-2-carrying Citrobacter koseri isolate collected from a patient with diarrhoea

Hu, Xinjun; Xu, Hao; Shang, Yibing; Guo, Lan-Ping; Song, Li; Zhang, Huping; Feng, Yue; Jiang, Hong; Zheng, Beiwen

doi:10.1016/j.jgar.2018.09.016

Cited by 7 publications

(5 citation statements)

References 6 publications

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“…Given that the in vitro conjugation experiments were unsuccessful, it was inferred that the size of the megaplasmid may be related to the ine cient transformation conjugation experiments to some extent (37,38). Unlike the previous literature that bla KPC gene was embedded on Tn4401 generally, a member of the Tn3 family transposon involved in gene acquisition and dissemination, bla KPC−3 gene with the linear structure IS6100-ISKpn27-bla KPC−3 -ISKpn6-korC-klcA in this work was found adjacent to another Tn3 family transposase TnAs1 (39,40). IS6100, belongs to the IS6 family members, is also one of the insertion sequences closely related to ARGs and can mediate sequence transfer and recombination between chromosomes or plasmids in different bacteria (41).…”

Section: Discussioncontrasting

confidence: 76%

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Qiao

et al. 2023

Preprint

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Background: Although Klebsiella pneumoniae Carbapenemase (KPC) -type class A β-lactamases spread widely throughout the world, KPC-3-producing isolates are rarely reported in China. This study aims to explore the emergence, antibiotic resistance profiles, and plasmid characteristics of blaKPC-3-carrying Pseudomonas aeruginosa. Methods: Species identification was performed by MALDI-TOF-MS, and antimicrobial resistance genes (ARGs) were identified by polymerase chain reaction (PCR). The characteristics of the target strain were detected by whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST). Plasmids were analyzed by S1-nuclease pulsed-field gel electrophoresis(S1-PFGE), Southern blotting and transconjugation experiment. Results: Five P. aeruginosa strains carrying blaKPC-3 were isolated from two Chinese patients without a history of travelling to endemic areas. All strains belonged to the novel sequence type ST1076. The blaKPC-3 was carried on a 395-kb IncP-2 megaplasmid with a conserved structure (IS6100-ISKpn27-blaKPC-3-ISKpn6-korC-klcA), and this genetic sequence was identical to many plasmid-encoded KPC of Pseudomonas species. By further analyzing the genetic context, it was supposed that the original of blaKPC-3 in our work was a series of mutation of blaKPC-2. Conclusions: The emergence of a multidrug resistance IncP-2 megaplasmid and clonal transmission of blaKPC-3-producing P. aeruginosa in China underlined the crucial need for continuous monitoring of blaKPC-3 for prevention and control of its further dissemination in China.

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Section: Discussioncontrasting

confidence: 76%

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Qiao

et al. 2023

Preprint

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“…Unlike the previous literature that bla KPC gene was embedded on Tn 4401 generally, a member of the Tn3 family transposon involved in gene acquisition and dissemination, bla KPC-3 gene with the linear structure IS 6100 -IS Kpn27 - bla KPC-3 -IS Kpn6 - korC - klcA in this work was found adjacent to another Tn 3 family transposase Tn As1 [ 43 , 44 ]. IS 6100 , belonging to the IS 6 family members, is also one of the insertion sequences closely related to ARGs and can mediate sequence transfer and recombination between chromosomes or plasmids in different bacteria [ 45 ].…”

Section: Discussioncontrasting

confidence: 68%

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Qiao

Zheng

et al. 2023

Ann Clin Microbiol Antimicrob

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Background Despite the global prevalence of Klebsiella pneumoniae Carbapenemase (KPC)-type class A β-lactamases, occurrences of KPC-3-producing isolates in China remain infrequent. This study aims to explore the emergence, antibiotic resistance profiles, and plasmid characteristics of blaKPC-3-carrying Pseudomonas aeruginosa. Methods Species identification was performed by MALDI-TOF-MS, and antimicrobial resistance genes (ARGs) were identified by polymerase chain reaction (PCR). The characteristics of the target strain were detected by whole-genome sequencing (WGS) and antimicrobial susceptibility testing (AST). Plasmids were analyzed by S1-nuclease pulsed-field gel electrophoresis(S1-PFGE), Southern blotting and transconjugation experiment. Results Five P. aeruginosa strains carrying blaKPC-3 were isolated from two Chinese patients without a history of travelling to endemic areas. All strains belonged to the novel sequence type ST1076. The blaKPC-3 was carried on a 395-kb IncP-2 megaplasmid with a conserved structure (IS6100-ISKpn27-blaKPC-3-ISKpn6-korC-klcA), and this genetic sequence was identical to many plasmid-encoded KPC of Pseudomonas species. By further analyzing the genetic context, it was supposed that the original of blaKPC-3 in our work was a series of mutation of blaKPC-2. Conclusions The emergence of a multidrug resistance IncP-2 megaplasmid and clonal transmission of blaKPC-3-producing P. aeruginosa in China underlined the crucial need for continuous monitoring of blaKPC-3 for prevention and control of its further dissemination in China.

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“…The global spread of the resistance of Enterobacterales to many antibiotics, seriously affecting the treatment of infections, has become a major public issue ( Hu et al., 2018 ; Dong et al., 2018 ; Dong et al., 2018 ; Zheng et al., 2020 ). The major plasmid types carrying bla KPC-2 published included IncFII, IncF, IncP-6, IncN, ColRNAI, and IncI2 ( Xu et al., 2018 ; Hu et al., 2019 ; Xiaoliang et al., 2019 ; Abril et al., 2021 ; Peng et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of two carbapenem-resistant Serratia marcescens isolates causing bacteremia: Emergence of KPC-2-encoding IncR plasmids

Jia

Huang²,

Zhang³

et al. 2023

Front. Cell. Infect. Microbiol.

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The occurrence and transmission of carbapenemase-producing-Enterobacterales (CPE) on a global scale has become a major issue. Clinical reports are rarely providing information on the genomic and plasmid features of carbapenem-resistant Serratia marcescens. Our objective was to investigate the resistance and transmission dynamics of two carbapenem-resistant S. marcescens that are resistant to carbapenem and have caused bacteremia in China. Blood specimens were taken from two individuals with bacteremia. Multiplex PCR was employed to identify genes that code for carbapenemase. Antimicrobial susceptibility tests and plasmid analysis were conducted on S. marcescens isolates SM768 and SM4145. The genome of SM768 and SM4145 were completely sequenced using NovaSeq 6000-PE150 and PacBio RS II platforms. Antimicrobial resistance genes (ARGs) were predicted using the ResFinder tool. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and southern blotting were employed to analyze plasmids. Two S. marcescens that produced KPC-2 were identified from bloodstream infections. The antimicrobial susceptibility testing demonstrated that both of the isolates had a resistance to various antibiotics. The whole-genome sequence (WGS) and plasmid analysis revealed the presence of blaKPC-2-bearing IncR plasmids and multiple plasmid-borne antimicrobial resistance genes in the isolates. Our comparative plasmid analysis suggested that the two IncR plasmids identified in this study could be derived from a common ancestor. Our findings revealed the emergence of blaKPC-2-bearing IncR plasmid in China, which could be a hindrance to the transmission of KPC-2-producing S. marcescens in clinical settings.

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First genome sequence of a blaKPC-2-carrying Citrobacter koseri isolate collected from a patient with diarrhoea

Cited by 7 publications

References 6 publications

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Emergence and clonal dissemination of KPC-3-producing Pseudomonas aeruginosa in China with an IncP-2 megaplasmid

Genomic characterization of two carbapenem-resistant Serratia marcescens isolates causing bacteremia: Emergence of KPC-2-encoding IncR plasmids

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