First frozen repository for the Great Barrier Reef coral created

Hagedorn, Mary; Oppen, Madeleine J. H. van; Carter, Virginia; Henley, Mike; Abrego, David; Puill-Stephan, Eneour; Negri, Andrew P.; Heyward, Andrew; MacFarlane, Douglas R.; Spindler, Rebecca

doi:10.1016/j.cryobiol.2012.05.008

Cited by 33 publications

(30 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rinkevich () highlighted the need to conserve the gene pool of reef building corals for possible future reseeding of lost reef areas. Conserving coral gene pool is motivating both in vitro (Hagedorn et al ., ) and in situ (Horoszowski‐Fridman et al ., ) actions, including ‘Assisted Colonization’, that is, transplantation of corals to identified ‘refugia’ (Hoegh‐Guldberg et al ., ) but see (Howells et al ., ). Here, we propose that the Gulf of Aqaba (GoA), northern Red Sea, is a natural refugium due to its latitudinal location, topography, oceanography, and especially its recent geological history.…”

Section: Introductionmentioning

confidence: 99%

A coral reef refuge in the Red Sea

Fine

Gildor

Genin

2013

Global Change Biology

218

209

View full text Add to dashboard Cite

The stability and persistence of coral reefs in the decades to come is uncertain due to global warming and repeated bleaching events that will lead to reduced resilience of these ecological and socio-economically important ecosystems. Identifying key refugia is potentially important for future conservation actions. We suggest that the Gulf of Aqaba (GoA) (Red Sea) may serve as a reef refugium due to a unique suite of environmental conditions. Our hypothesis is based on experimental detection of an exceptionally high bleaching threshold of northern Red Sea corals and on the potential dispersal of coral planulae larvae through a selective thermal barrier estimated using an ocean model. We propose that millennia of natural selection in the form of a thermal barrier at the southernmost end of the Red Sea have selected coral genotypes that are less susceptible to thermal stress in the northern Red Sea, delaying bleaching events in the GoA by at least a century.

show abstract

Section: Introductionmentioning

confidence: 99%

A coral reef refuge in the Red Sea

Fine

Gildor

Genin

2013

Global Change Biology

218

209

View full text Add to dashboard Cite

show abstract

“…Coral reefs are a clear example of the need of cryopreservation for conservation purposes. Human activities have a deep impact in coral reefs degradation, and successful cryopreservation protocols could guarantee genotypes preservation until habitats could be rehabilitated (Hagedorn et al, 2012). Regarding aquaculture industry, cryopreservation would contribute to reduce broodstock cost and to have a fine control of several reproductive aspects (Adams et al, 2004).…”

Section: Germplasm Cryobanking Of Invertebratesmentioning

confidence: 99%

“…From a conservation point of view, efforts towards coral biodiversity preservation yield promising results, and the first frozen repository of coral has been created (Hagedorn et al, 2012). The establishment of genetic banks will undoubtedly help in the conservation of valuable areas such as the Great Barrier Reef (Hagedorn and Spindler, 2014).…”

Section: Germplasm Cryobanking Of Invertebratesmentioning

confidence: 99%

“…At this time, the bank maintains germplasm from a number of coral species from the Great Barrier Reef (Hagedorn and Spindler, 2014; Hagedorn et al, 2012) as well as from Dugong ( Dugong dugon ) and these are the only aquatic species in the bank. The CryoReserve also participates in projects investigating sperm cryobiology of other “at risk” species.…”

Section: Cryobanking Worldwidementioning

confidence: 99%

See 1 more Smart Citation

Cryobanking of aquatic species

et al. 2017

View full text Add to dashboard Cite

This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

show abstract

“…Cryopreservation of coral sperm and fertilization of coral eggs with thawed cryopreserved sperm have both been successfully demonstrated in the past (Hagedorn et al 2012a;Hagedorn et al 2012b;Hagedorn et al 2017). Coral sperm repositories in the U.S. and Australia (Hagedorn et al 2012b) now hold material from over 25 coral species.…”

Section: Introductionmentioning

confidence: 99%

Successful Demonstration of Assisted Gene Flow in the Threatened Coral Acropora Palmata Across Genetically-Isolated Caribbean Populations Using Cryopreserved Sperm

Hagedorn

Page

O'Neill

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Global change will compromise the population sizes, species ranges, and survival of economically-important plants and animals, including crops, aquaculture species, and foundational ecosystem builders. Scleractinian reef-building corals are a particular concern because they are slow-growing, long-lived, environmentally-sensitive, and concentrated in the warmest regions of the ocean. Assisted Gene Flow (AGF) is considered a viable tool to help natural plant and animal populations, including corals, adapt to changing environments. Our goal was to test for the first time whether cryopreserved coral sperm could be used to facilitate assisted gene flow between genetically-isolated populations of a Caribbean coral. We collected, pooled, and cryopreserved coral sperm from the threatened Caribbean coral Acropora palmata in the western Caribbean (Key Largo, FL), central Caribbean (Rincón, Puerto Rico), and eastern Caribbean (Curaçao). Alongside freshly-collected sperm from Curaçao, the cryopreserved sperm from each of these populations was used for in vitro fertilization experiments with freshlycollected eggs from Curaçao. Across five egg donors, average fertilization success was 91 to 99% for CUR × CUR (fresh sperm) crosses, 37 to 82% for CUR × CUR (frozen sperm) crosses, 3 to 19% for CUR × FL (frozen sperm) crosses and 0 to 24% for CUR × PR (frozen sperm) crosses. Notably, fertilization was achieved in all four categories of crosses, showing for the first time through direct evidence that populations of A. palmata are reproductively compatible, and that genetic diversity can be transferred from one population to another for the purposes of assisted gene flow. The resulting larvae were reared in Curaçao for up to 7 days, then the swimming larvae were transported to Florida for settlement and grow-out at two separate facilities, which achieved larval settlement rates of 37 to 60% across all cohorts. Larvae were reared and settled in Florida to acclimate them to the ambient water quality, microbial environment, and temperature regimes of the western genetic A. palmata population as early in their life cycle as possible. At one month, over 54% all settlers had survived, including over 3500 settlers from CUR x CUR (frozen sperm), 1200 settlers from CUR × FL (frozen sperm), and 230 settlers from CUR × PR (frozen sperm). These experiments represent the first-ever pan-Caribbean coral crosses produced in captivity and the first direct evidence that geographicallyseparated and genetically-isolated populations of any Caribbean coral are reproductively compatible. Moreover, with over 4700 A. palmata settlers produced using cryopreserved sperm, Hagedorn et al. Cryopreservation-Assisted Gene Flow in Elkhorn Coral 3 this represents the largest living wildlife population ever created from cryopreserved material. Together, these findings demonstrate that cryopreservation of coral sperm can enable efficient, large-scale assisted gene flow in corals. This form of assisted migration can not only help to preserve the population-level geneti...

show abstract

First frozen repository for the Great Barrier Reef coral created

Cited by 33 publications

References 10 publications

A coral reef refuge in the Red Sea

A coral reef refuge in the Red Sea

Cryobanking of aquatic species

Successful Demonstration of Assisted Gene Flow in the Threatened Coral Acropora Palmata Across Genetically-Isolated Caribbean Populations Using Cryopreserved Sperm

Contact Info

Product

Resources

About