First Experimental Evidence for the Presence of Potentially Toxic Vibrio cholerae in Snails, and Virulence, Cross-Resistance and Genetic Diversity of the Bacterium in 36 Species of Aquatic Food Animals

Chen, Dailing; Li, Xiangyi; Ni, Ling; Xu, Dingxiang; Xu, Yingwei; Ding, Yong; Xie, Lu; Chen, Lanming

doi:10.3390/antibiotics10040412

Cited by 10 publications

(27 citation statements)

References 55 publications

(89 reference statements)

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“…Many of the genes encoding antibiotic resistance help to enhance fitness and survival of VC enabling it to tide over the deleterious antibiotics (31,114). These genetic exchanges have been facilitated by the ecological interactions of these organisms in different ecosystems like the gut and the aquatic ecosystem which is the natural habitat of VC (14,15,39,144).…”

Section: The Role Of the Microbiome In The Evolution Of The Acquired Resistome Of V Choleraementioning

confidence: 99%

“…its virulence and MDR traits (46,185,186). Therefore, it plays a crucial role in determining virulence, pathogenicity and its response to antibiotics (14,185). Genetic exchange in the microbiota does not occur randomly (187).…”

Section: The Role Of the Microbiome In The Evolution Of The Acquired Resistome Of V Choleraementioning

confidence: 99%

“…Common bacterial pathogens have developed resistance to antibiotics including broad-spectrum ones belonging to all the major classes of antibiotics which have been empirically used for their treatment (12). The problem of AMR in VC is growing at a threatening pace (14). It is a common food-borne pathogen and the most common water-borne pathogen indicating its high chances of infecting the human population (14).…”

Section: Introductionmentioning

confidence: 99%

“…The problem of AMR in VC is growing at a threatening pace (14). It is a common food-borne pathogen and the most common water-borne pathogen indicating its high chances of infecting the human population (14). Its resistance profile has been cautionary and calls for immediate action to revert the AMR crisis in VC (14,15).…”

Section: Introductionmentioning

confidence: 99%

“…It is a common food-borne pathogen and the most common water-borne pathogen indicating its high chances of infecting the human population (14). Its resistance profile has been cautionary and calls for immediate action to revert the AMR crisis in VC (14,15). AMR in VC was not common till the early 1970s when the first reports on resistance in VC started emerging (16).…”

Section: Introductionmentioning

confidence: 99%

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Mobile Genetic Elements of Vibrio cholerae and the Evolution of Its Antimicrobial Resistance

2021

Front. Trop. Dis

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Vibrio cholerae (VC) is the causative agent of the severe dehydrating diarrheal disease cholera. The primary treatment for cholera is oral rehydration therapy (ORT). However, in case of moderate to severe dehydration, antibiotics are administered to reduce morbidity. Due to the emergence of multidrug resistant (MDR) strains of VC routinely used antibiotics fail to be effective in cholera patients. Antimicrobial resistance (AMR) is encoded in the genome of bacteria and is usually acquired from other organisms cohabiting in the environment or in the gut with which it interacts in the gut or environmental niche. The antimicrobial resistance genes (ARGs) are usually borne on mobile genetic elements (MGEs) like plasmids, transposons, integrons and SXT constin. Horizontal gene transfer (HGT) helps in the exchange of ARGs among bacteria leading to dissemination of AMR. In VC the acquisition and loss of AMR to many antibiotics have been found to be a dynamic process. This review describes the different AMR determinants and mechanisms of resistance that have been discovered in VC. These ARGs borne usually on MGEs have been recovered from isolates associated with past and present epidemics worldwide. These are responsible for resistance of VC to common antibiotics and are periodically lost and gained contributing to its genetic evolution. These resistance markers can be routinely used for AMR surveillance in VC. The review also presents a precise perspective on the importance of the gut microbiome in the emergence of MDR VC and concludes that the gut microbiome is a potential source of molecular markers and networks which can be manipulated for the interception of AMR in the future.

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Section: The Role Of the Microbiome In The Evolution Of The Acquired Resistome Of V Choleraementioning

confidence: 99%

Section: The Role Of the Microbiome In The Evolution Of The Acquired Resistome Of V Choleraementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mobile Genetic Elements of Vibrio cholerae and the Evolution of Its Antimicrobial Resistance

2021

Front. Trop. Dis

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Metal contamination and coexistence of metal and antibiotic resistance in Vibrio species recovered from aquaculture ponds with and without history of antibiotic usage in Southwest Nigeria

2021

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Background The use of metal-containing and antimicrobial compounds is a common practice in aquaculture operations in Nigeria. Only a few studies have focused on the impact of these chemicals on metal and antibiotic resistance development in the bacterial flora of aquaculture. This study therefore sets out to fill this gap. Results Five metals, namely copper (Cu), zinc (Zn), lead (Pb), nickel (Ni) and chromium (Cr), were detected at different concentrations in the pond water samples, with most values obtained exceeding the maximum recommended national standard for fisheries and recreation water quality criteria set by the National Environmental Standards and Regulations Enforcement Agency (NESREA) in Nigeria. A total of 121 Vibrio spp. were recovered from the aquaculture pond water with Vibrio cholerae having the highest frequency (37) followed by Vibrio parahaemolyticus (36), Vibrio vulnificus (36) and Vibrio alginolyticus (12). Seventy-five isolates were recovered from ponds with history of antibiotic use, while the ponds with no recognized history of antibiotic use had forty-six isolates. The percentage resistance to antibiotics in the isolates was: ampicillin (88.5%), amoxicillin–clavulanate (44.3%), tetracycline (41%), trimethoprim-sulfamethoxazole (22.1%), chloramphenicol (15.6%), ciprofloxacin (11.5%), imipenem (5.7%), ceftazidime (4.1%) and gentamicin (0%). All the isolates tolerated all the metals to 400 µg/mL concentration while only the trio of Pb, Ni and Cr were tolerated beyond the concentration. Zn and Cu appeared to be the most toxic metals on Vibrio spp., with no isolate surviving beyond the 400 µg/mL concentration mark, while 57 of the 121 isolates survived Cr at the highest concentration (3200 µg/mL), making it the most tolerated of the metals. Conclusion This study reports a high level of metal contamination in the aquaculture ponds studied. The concentration of the metals exceeded the maximum permissible limits in Nigeria. Also, there was coexistence of metal and antibiotic resistance in the bacteria recovered from the aquaculture ponds. There is a need to regulate the use of chemicals, especially antibiotics and some biocidal compounds, to clamp down on the introduction of metals and antibiotics into the aquaculture systems, as these chemicals could facilitate the development of resistance traits in aquaculture bacteria by creating a selective pressure in the aquaculture environment.

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Diverse Aquatic Animal Matrices Play a Key Role in Survival and Potential Virulence of Non-O1/O139 Vibrio cholerae Isolates

et al. 2022

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Vibrio cholerae can cause pandemic cholera in humans. The waterborne bacterium is frequently isolated from aquatic products worldwide. However, current literature on the impact of aquatic product matrices on the survival and pathogenicity of cholerae is rare. In this study, the growth of eleven non-O1/0O139 V. cholerae isolates recovered from eight species of commonly consumed fish and shellfish was for the first time determined in the eight aquatic animal matrices, most of which highly increased the bacterial biomass when compared with routine trypsin soybean broth (TSB) medium. Secretomes of the V. cholerae isolates (draft genome size: 3,852,021–4,144,013 bp) were determined using two-dimensional gel electrophoresis (2DE-GE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques. Comparative secretomic analyses revealed 74 differential extracellular proteins, including several virulence- and resistance-associated proteins secreted by the V. cholerae isolates when grown in the eight matrices. Meanwhile, a total of 8,119 intracellular proteins were identified, including 83 virulence- and 8 resistance-associated proteins, of which 61 virulence-associated proteins were absent from proteomes of these isolates when grown in the TSB medium. Additionally, comparative genomic and proteomic analyses also revealed several strain-specific proteins with unknown functions in the V. cholerae isolates. Taken, the results in this study demonstrate that distinct secretomes and proteomes induced by the aquatic animal matrices facilitate V. cholerae resistance in the edible aquatic animals and enhance the pathogenicity of the leading waterborne pathogen worldwide.

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First Experimental Evidence for the Presence of Potentially Toxic Vibrio cholerae in Snails, and Virulence, Cross-Resistance and Genetic Diversity of the Bacterium in 36 Species of Aquatic Food Animals

Cited by 10 publications

References 55 publications

Mobile Genetic Elements of Vibrio cholerae and the Evolution of Its Antimicrobial Resistance

Mobile Genetic Elements of Vibrio cholerae and the Evolution of Its Antimicrobial Resistance

Metal contamination and coexistence of metal and antibiotic resistance in Vibrio species recovered from aquaculture ponds with and without history of antibiotic usage in Southwest Nigeria

Diverse Aquatic Animal Matrices Play a Key Role in Survival and Potential Virulence of Non-O1/O139 Vibrio cholerae Isolates

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