First Evaluation of the Biologic Effectiveness Factors of Boron Neutron Capture Therapy (BNCT) in a Human Colon Carcinoma Cell Line

Comparative study of the radiobiological effects induced on adherent vs suspended cells by BNCT, neutrons and gamma rays treatments

“…The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay, a well known test usually used to study chemosensitivity or toxicity, was compared to clonogenic assay resulting in a good correlation (Buch et al, 2012;Kawada et al, 2002). MTT test performed after irradiation of adherent cells was applied also to cell survival studies after BNCT treatment (Dagrosa et al, 2011). The use of different laboratory protocols for in vitro cell irradiation can have consequences in the calculation of the dose, making it difficult to compare results (Mackonis et al, 2012).…”

Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: A boron neutron capture therapy study

“…A comparable study with 9L glioma cells treated with sodium borocaptate (BSH) resulted in similar control and 10 B survival curves with approximately 10 μ g 10 B/10 9 cells (Matsumura et al 1999). Additionally, a study of human colon carcinoma cells with similar dose components yielded comparable survival curves within the neutron fl uence region used in this study (Dagrosa et al 2011). In neutron beams with increased epithermal and fast neutron contribution to the energy spectrum, a decrease in survival of both control and 10 B-treated groups has been observed, likely resulting from signifi cant dose contribution of proton recoil, when compared to the MUTR experiments (Fairchild et al 1990, Tilly et al 1996.…”

Neutron Exposures in Human Cells: Bystander Effect and Relative Biological Effectiveness

“…Yang et al [71] reported RBEs of 2.35 and 2.42 for fast neutrons in immature rat hippocampal cells, as determined by two different cell viability assays. Dagrosa et al [72] used the cytokinesis-block micronucleus assay and a cell survival assay in a human colon carcinoma cell line and observed an RBE of 4.4 for neutrons in boron neutron capture therapy. RBEs for neutrons as low as 4 to as high as 63 have been reported after measuring life-shortening responses in mice [73], apoptosis [1] and induction of dicentrics [74] in human lymphocytes.…”