First Environmental Dna (Edna) Record of Central Amazon in a Floodplain Lake: Extraction Method Selection and Validation / Primeiro Registro De Dna Ambiental (Edna) Da Amazônia Central Em Um Lago De Várzea: Seleção E Validação De Método De Extração

Bevilaqua, Danniel Rocha; Melo, Sabrina Araújo de; Freitas, Carlos Edwar de Carvalho; Silva, Ana Caroline Viana da; Batista, Jacqueline da Silva

doi:10.34117/bjdv6n11-254

BJD

2020

DOI: 10.34117/bjdv6n11-254

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First Environmental Dna (Edna) Record of Central Amazon in a Floodplain Lake: Extraction Method Selection and Validation / Primeiro Registro De Dna Ambiental (Edna) Da Amazônia Central Em Um Lago De Várzea: Seleção E Validação De Método De Extração

Danniel Rocha Bevilaqua

Sabrina Araújo de Melo

Carlos Edwar de Carvalho Freitas

et al.

Abstract: First environmental DNA (eDNA) record of central Amazon in a floodplain lake: extraction method selection and validation Primeiro registro de DNA ambiental (eDNA) da Amazônia central em um lago de várzea: seleção e validação de método de extração

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Electric organ discharge from electric eel facilitates DNA transformation into teleost larvae in laboratory conditions

Sakaki,

Ito,

Abe

et al. 2023

PeerJ

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Background Electric eels (Electrophorus sp.) are known for their ability to produce electric organ discharge (EOD) reaching voltages of up to 860 V. Given that gene transfer via intense electrical pulses is a well-established technique in genetic engineering, we hypothesized that electric eels could potentially function as a gene transfer mechanism in their aquatic environment. Methods To investigate this hypothesis, we immersed zebrafish larvae in water containing DNA encoding the green fluorescent protein (GFP) and exposed them to electric eel’s EOD. Results and Discussion Some embryos exhibited a mosaic expression of green fluorescence, in contrast to the control group without electrical stimulation, which showed little distinct fluorescence. This suggests that electric eel EOD has the potential to function as an electroporator for the transfer of DNA into eukaryotic cells. While electric eel EOD is primarily associated with behaviors related to sensing, predation, and defense, it may incidentally serve as a possible mechanism for gene transfer in natural environment. This investigation represents the initial exploration of the uncharted impact of electric eel EOD, but it does not directly establish its significance within the natural environment. Further research is required to understand the ecological implications of this phenomenon.

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Electric organ discharge from electric eel facilitates DNA transformation into teleost larvae in laboratory conditions

Sakaki,

Ito,

Abe

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

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First Environmental Dna (Edna) Record of Central Amazon in a Floodplain Lake: Extraction Method Selection and Validation / Primeiro Registro De Dna Ambiental (Edna) Da Amazônia Central Em Um Lago De Várzea: Seleção E Validação De Método De Extração

Abstract: First environmental DNA (eDNA) record of central Amazon in a floodplain lake: extraction method selection and validation Primeiro registro de DNA ambiental (eDNA) da Amazônia central em um lago de várzea: seleção e validação de método de extração

Cited by 1 publication

References 22 publications

Electric organ discharge from electric eel facilitates DNA transformation into teleost larvae in laboratory conditions

Electric organ discharge from electric eel facilitates DNA transformation into teleost larvae in laboratory conditions

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