First Detection of Faba bean necrotic yellow virus in Spain

Abstract

Faba bean necrotic yellow virus (FBNYV) was not detected during 1994 to 1996 field surveys of faba beans (Vicia faba L.) in Spain (1). In 1997, however, one sample with symptoms of necrosis, collected in Baleares, was tested using ELISA (enzyme-linked immunosorbent assay) and was positive for both Tomato spotted wilt virus (TSWV) and FBNYV. FBNYV is a single-strand DNA virus that is transmitted by aphids and is the main virus disease of broad bean in North Africa and West Asia (2). During 1997 to 1999, faba bean plants with symptoms of necrosis, yellowing, small leaves, and stunting were collected from several fields in the Murcia Region (Spain) and were analyzed using ELISA. To detect FBNYV, we used monoclonal 2E9 supplied by H. J. Vetten (Institute of Plant Virology, Microbiology and Biosafety, BBA, Braunschweig, Germany). Of 700 samples analyzed, 34 were positive for FBNYV. Of the 34 positive samples, 12 tested positive, using commercial antiserum from Loewe, Inc. (Munich) for mixed infections with TSWV. FBNYV was transmitted to healthy faba bean plants by aphids (Acyrthosiphon pisum) in greenhouse experiments and was confirmed by ELISA. Preliminary epidemiological data showed a gradual increase in the number of plants infected with time in the same field. Aphid transmission of FBNYV to faba beans has established the disease in Spain and is a potential threat to other leguminous crops. This is the first report of a nanovirus in Europe. References: (1) J. Fresno et al. Plant Dis. 81:112, 1997. (2) L. Katul et al. Ann. Appl. Biol. 123:629, 1993.

Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain

“…In the 1991/1992 growing season, the virus occurred on an epidemic scale on faba beans in Egypt to complete crop failure (Makkouk et al, 1994). In Spain FBNYV was first detected in 1997 in a faba bean sample collected at Balearic Islands and in 34 samples collected during 1997-1999 in Murcia (Babín et al, 2000). The present work reports that recent surveys in the main Spanish legume-producing areas showed FBNYV to be present only in Murcia.…”

“…The presence of FBNYV was reported for the first time in Spain a few years ago (Babín et al, 2000). The present paper examines the distribution of FBNYV in the main legume-producing areas of Spain and its transmission efficiency by two aphid vectors.…”

Control of Plant Virus Diseases in Cool-Season Grain Legume Crops

“…Sa, Se, Aphids P Is, 28 As, Af Farzadfar and Izadpanah (2001), Fortass and Bos (1991), , Makkouk, Kumari, and Bayaa (1999), Makkouk, Kumari, Sarker, and Erskine (2001), Makkouk, Hamed, Hussein, et al (2003), Makkouk, Kumari, Shahraeen, et al (2003), Peters (1982), Tadesse et al (1999) Pea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) FB, P, Cp, Le Sa, Se, Aphids NP Fi, 770 As, Af, Au Abraham et al (2000), Allam et al (1979), Bayaa et al (1998), Fortass and Bos (1991), Hampton and Mink (1975), Kaiser et al (1968), , Latham and Jones (2001b), , Makkouk, Bos, Horn, and Srinivasa Rao (1993), Makkouk, Bashir, Jones, et al (2001), Mustafayev et al (2011), Shiying et al (2007, Tadesse et al (1999) Continued Johnstone, Liu, and Duffus (1984), Johnstone and Guy (1986), Makkouk et al (1997), Makkouk, Kumari, and van Leur (2002), Makkouk, Kumari, Shahraeen, et al (2003), Najar, Kumari, Makkouk, and Daaloul (2003), Tadesse et al (1999) , and several other viruses are widespread in some of these crops in specific regions within specific countries (e.g., Abraham et al, 2006;Ait Yahia, Aitouada, Hadj Arab, Belfendess, & Sarni, 1997;Ait Yahia, Aitouada, Illoul, et al, 1997;Babin, Ortiz, Castro, & Romero, 2000;Bayaa et al, 1998;…”

Genome diversity and evidence of recombination and reassortment in nanoviruses from Europe

“…Whereas babuviruses appear to be restricted to wild and cultivated Musa and cardamom species in Africa, Australia, South Asia and Pacific islands (Thomas, 2008), nanoviruses have been reported from a wide variety of legume species in an increasing number of countries throughout North and Eastern Africa, the Middle East, China, Japan, Australia (Abraham et al , 2010, 2012; Makkouk & Kumari, 2009; Vetten, 2008) and even Europe, where faba bean necrotic yellows virus (FBNYV) was first reported from Spain in 2000 (Babín et al , 2000) and from Azerbaijan in 2009 (Kumari et al , 2009). Recently, we identified a novel nanovirus, pea necrotic yellow dwarf virus (PNYDV), in commercially grown pea ( Pisum sativum L.) in Germany (Grigoras et al , 2010a).…”