First Detection of Equine Anaplasmosis and Hemoplasmosis of Horses in Mosul City, Iraq

Al-Badrani, B. A.; Al-Iraqi, Osamah Muwaffag

doi:10.17582/journal.aavs/2019/7.2.106.111

Cited by 4 publications

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“…detached from cattle. In addition, [ 45 ] reported a higher prevalence of Anaplasma phagocytophilium (51%) in hemolymph smears prepared from Hyalomma spp. collected from horses in Iraq.…”

Section: Discussionmentioning

confidence: 99%

Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt

Hegab¹,

Omar

Abuowarda

et al. 2022

Parasites Vectors

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Background The incidence or recurrence of tick-borne diseases (TBDs) in animals and humans is increasing rapidly worldwide, but there is insufficient information about TBDs infecting dogs in Egypt. Thus, the present study was conducted to screen and genetically identify tick-borne pathogens (TBPs) in dogs and associated ticks by microscopic examination and polymerase chain reaction (PCR). Methods In Cairo and Giza governorates, 208 blood samples were collected from dogs of different breeds, ages, and sex. In addition, 1266 dog-associated ticks were collected (546 ticks were used to prepare hemolymph smears, and 720 ticks were kept in 70% ethanol until PCR analysis). PCR was applied to 124 dog blood samples and 144 tick pools prepared from 720 ticks. Results All ticks collected from dogs were Rhipicephalus sanguineus (s.l.). Microscopic examination revealed that TBP prevalence among dogs was 23.56% (49/208), including Anaplasma and Ehrlichia with 11.1% (23/208) and Babesia canis with 8.2% (17/208). Hepatozoon canis was not detected in blood smears. Co-infections with two pathogens were visible in 4.33% (9/208) of examined dogs. The prevalence of TBPs in hemolymph smears was 45.97% (251/546) including 35.89% (196/546) for H. canis, 8.1% (44/546) for B. canis, and 2.01% (11/546) for Anaplasmataceae (A. phagocytophilum, A. marginale, A. platys, and E. canis). The overall molecular prevalence rate of TBPs was 25.81% and 29.17% in the blood of examined dogs and in ticks, respectively. The molecular prevalence of Anaplasmataceae family, Babesia canis, and H. canis in dog blood samples was 19.35%, 6.45%, and 0.0%, respectively, while in ticks, it was 20.83%, 5.55%, and 2.8%, respectively. A sequential analysis identified six different species of TBPs, namely B. canis vogeli, Hepatozoon canis, A. phagocytophilum, A. marginale, A. platys, and E. canis. The obtained sequences were submitted to GenBank and assigned accession numbers. Conclusions The present study detected a wide range of TBPs (B. canis, H. canis, A. platys, A. phagocytophilum, A. marginale, and E. canis) that are considered a threat to domestic animals and humans in Egypt. Hepatozoon canis and A. marginale were reported in dogs and associated ticks for the first time in Egypt. Graphical Abstract

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“…detached from cattle. In addition, [ 45 ] reported a higher prevalence of Anaplasma phagocytophilium (51%) in hemolymph smears prepared from Hyalomma spp. collected from horses in Iraq.…”

Section: Discussionmentioning

confidence: 99%

Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt

Hegab¹,

Omar

Abuowarda

et al. 2022

Parasites Vectors

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“…In Iraq, the occurrence of Anaplasma spp. in domestic ruminants has also been documented (3,18,19). Data concerning the presence, distribution, and molecular typing of Anaplasma spp.…”

Section: Introductionmentioning

confidence: 91%

“…Bovine anaplasmosis is a tick-borne infectious disease caused by five species of the genus Anaplasma (family Anaplasmaceae), including Anaplasma centrale, A. marginale, A. bovis, A. phagocytophilum and A. platys that circulate in cattle worldwide (1). All species infect blood cells of a variety of domestic and wild animals, as well as humans and show various cell affinity (2,3). Bovine anaplasmosis causes significant economic losses through reduction in meat and milk production and high mortality rates in cattle (4).…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Phylogenetic Analysis of Anaplasma Spp. in Cattle in Al-Qadisiyah Province of Iraq

Ayyez

Khudhair

Kshash

2019

Macedonian Veterinary Review

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Anaplasma spp. are widely spread rickettsial bacteria transmitted by ticks and placing high impacts on veterinary and public health. A limited number of studies have been carried out on Anaplasmosis in the central part of Iraq. This study was conducted to determine the presence of Anaplasma spp. in cattle in Al-Qadisiyah province, Iraq. A total of 400 blood specimens were collected from cattle suffering from heavy tick infestation. Cattle were blood-sampled from four hyper-endemic areas with ticks. Blood samples were screened using microscopic and polymerase chain reaction (PCR) methods. Diff-quick stained blood smears revealed Anaplasma-like inclusion bodies in 254 (63.5%) samples. According to the 16S rRNA-gene-based PCR analysis, Anaplasma spp. was detected in 124 of the 400 (31%) samples, divided as 96/254 (37.8%) among the microscopical positive samples and 28/146 (19.17%) among the microscopical negative samples. Phylogenetic analysis based on the partial 16S rRNA gene sequencing of ten-PCR positive samples were 99–97% identical to sequences deposited in the GenBank, revealing presence of A. phagocytophilum, A. marginale and unnamed Anaplasma spp. in 40%, 20%, and 40% samples, respectively. Relationships among Anaplasma spp. infections and cattle breed, age, and sex were analyzed. Calves less than one year old showed significantly higher rates (p<0.005) than those from other age groups, whereas sex and breed demonstrated no significant differences (p˃0.001). This study shows that a variety of Anaplasma spp., were endemic in central part of Iraq and is still a hidden problem in cattle in the hyperendemic areas of tick, which requires serious control strategies.

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Equine Granulocytic Anaplasmosis

Oliver

Conrado

Nolen-Walston

2023

Veterinary Clinics of North America: Equine Practice

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First Detection of Equine Anaplasmosis and Hemoplasmosis of Horses in Mosul City, Iraq

Cited by 4 publications

References 0 publications

Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt

Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt

Molecular Detection and Phylogenetic Analysis of Anaplasma Spp. in Cattle in Al-Qadisiyah Province of Iraq

Equine Granulocytic Anaplasmosis

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