First detection of CMY-2 plasmid mediated β-lactamase in Salmonella Heidelberg in South America

Cejas, Daniela; Vignoli, Rafael; Quinteros, Mirta; Marino, Ricardo; Callejo, Raquel; Betancor, Laura; Gutkind, Gabriel; Radice, Marcela

doi:10.1016/s0325-7541(14)70044-6

Cited by 13 publications

(10 citation statements)

References 13 publications

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“…Among them, some isolates showed resistance to cephamycin (FOX) or fourth-generation cephalosporins (FEP), which suggests these strains are likely producing plasmid-mediated AmpC β-lactamases or extended spectrum β-lactamases (CTX-M-type), respectively. Similar results have been reported in S. Heidelberg isolates from humans (Cejas et al, 2014) and poultry (Castellanos et al, 2018;Souza et al, 2020). Moreover, the isolates show high prevalence of resistance to nalidixic acid and ciprofloxacin (FQ), a fact also observed by other authors in isolates from poultry (Casas et al, 2016;van den Berg et al, 2019).…”

Section: Discussionsupporting

confidence: 86%

Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

Dominguez

Viñas

Herrera

et al. 2021

Zoonoses and Public Health

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Section: Discussionsupporting

confidence: 86%

Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

Dominguez

Viñas

Herrera

et al. 2021

Zoonoses and Public Health

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“…The bla CMY-2 gene is the most common AmpC-type gene found in both E. coli and Salmonella from various sources: food, animals, and hospitals in multiple countries (Mulvey et al, 2005;Hiki et al, 2013;Cejas et al, 2014;Guo et al, 2014;Ghodousi et al, 2015). This gene has been hypothesized to have originated on the chromosome of E. coli and it could be induced with betalactams in some Enterobacteriaceae such as Enterobacter cloacae, Citrobacter freundii, Serratia marcescens, and Pseudomonas aeruginosa (Sanders, 1987;Philippon et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Unlike these bacteria, E. coli and Salmonella lack systems to produce inducible AmpC enzymes. Mutations in the ampC promoter have increased the resistance to oxyimino-cephalosporins in E. coli (Caroff et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of AmpC and Extended-Spectrum Beta-Lactamase Phenotypes in Escherichia coli and Salmonella From Alberta Broiler Chickens

Tran

Checkley

Caffrey

et al. 2021

Front. Cell. Infect. Microbiol.

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Horizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 Escherichia coli and 62 Salmonella enterica subsp. enterica isolates were isolated from broiler chicken farms in Alberta. Fourteen serovars were identified among Salmonella isolates. Thirty one percent of E. coli isolates (37/120) were multiclass drug resistant (resistant to ≥ 3 drug classes), while only about 16% of Salmonella isolates (10/62) were multiclass drug resistant. Among those, eight E. coli isolates had an AmpC-type phenotype, and one Salmonella isolate had an extended-spectrum beta-lactamase (ESBL)-type beta-lactamase phenotype. We identified both AmpC-type (blaCMY-2) and ESBL-type (blaTEM) genes in both E. coli and Salmonella isolates. Plasmids from eight of nine E. coli and Salmonella isolates were transferred to recipient strain E. coli J53 through conjugation. Transferable plasmids in the eight E. coli and Salmonella isolates were also transferred into a lab-made sodium azide-resistant Salmonella recipient through conjugation. The class 1 integrase gene, int1, was detected on plasmids from two E. coli isolates. Further investigation of class 1 integron cassette regions revealed the presence of an aadA gene encoding streptomycin 3’’-adenylyltransferase, an aadA1a/aadA2 gene encoding aminoglycoside 3’’-O-adenyltransferase, and a putative adenylyltransferase gene. This study provides some insight into potential horizontal gene transfer events of antimicrobial resistance genes between E. coli and Salmonella in broiler chicken production.

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“…Most of these isolates originated from ten different cities of two adjacent states from the Southern part of Brazil, Paraná (n = 79) and Santa Catarina (n = 40) and were all but one assigned ST15, a sequence type commonly identified among S . Heidelberg strains and often reported in Asia, Europe and the Americas in both food and human isolates [5, 6, 29]. Interestingly, the only exception was the most distant isolate relative to the other isolates from Brazil that was assigned ST4632 based on a single SNP in one of the MLST genes.…”

Section: Discussionmentioning

confidence: 99%

Characterization and whole genome sequencing of closely related multidrug-resistant Salmonella enterica serovar Heidelberg isolates from imported poultry meat in the Netherlands

et al. 2019

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Multidrug-resistant Salmonella enterica serovar Heidelberg isolates are frequently recovered in the Netherlands from poultry meat imported from South America. Our aim was to retrospectively assess the characteristics of the antimicrobial determinants, gene content and the clonal relatedness of 122 unique S . Heidelberg isolates from chicken meat from Brazil (n = 119) and Argentina (n = 3) that were imported between 2010 and 2015. These isolates were subjected to antimicrobial susceptibility testing, PCR and Illumina HiSeq2500 whole genome sequencing. Draft genomes were assembled to assess the gene content, and the phylogenetic relationships between isolates were determined using single nucleotide polymorphisms. Ciprofloxacin-resistance was identified in 98.4% of the isolates and 83.7% isolates showed resistance to the extended-spectrum cephalosporins cefotaxime and ceftazidime (83.6% and 82.8% respectively). Of the latter, 97.1% exhibited an AmpC phenotype and contained bla CMY-2 , whereas the remaining three isolates contained an extended spectrum beta-lactamase. Of the 99 extended-spectrum cephalosporins-resistant isolates harboring CMY-2 plasmids, 56.6% contained the incompatibility group I1 replicon. Phylogenetic cluster analysis showed that all isolates from Brazil clustered together, with 49% occurring in clusters larger than 5 isolates that revealed intra-cluster similarities based on geographical location and/or resistance profiles. The remaining isolates were classified in smaller clusters or as singletons, highlighting the large diversity of S . Heidelberg in the poultry chain in Brazil that was revealed by this study. Considering the potential public health risk associated with multidrug-resistant S . Heidelberg in imported poultry, collaborative whole genome sequencing-based surveillance is needed to monitor the spread, pathogenic properties and epidemiological distribution of these isolates.

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First detection of CMY-2 plasmid mediated β-lactamase in Salmonella Heidelberg in South America

Cited by 13 publications

References 13 publications

Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

Genetic Characterization of AmpC and Extended-Spectrum Beta-Lactamase Phenotypes in Escherichia coli and Salmonella From Alberta Broiler Chickens

Characterization and whole genome sequencing of closely related multidrug-resistant Salmonella enterica serovar Heidelberg isolates from imported poultry meat in the Netherlands

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