First Detection and Genomic Insight into mcr-1 Encoding Plasmid-Mediated Colistin-Resistance Gene in Escherichia coli ST101 Isolated from the Migratory Bird Species Hirundo rustica in Thailand

Aeksiri, Niran; Toanan, Wansane; Sawikan, Sirinan; Suwannarit, Rujikorn; Pungpomin, Prompong; Khieokhajonkhet, Anurak; Niumsup, Pannika R.

doi:10.1089/mdr.2019.0020

Cited by 19 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This gene was found in Enterobacteriaceae from rooks throughout the European continent [ 55 ] and human clinical isolates from Korea [ 56 ]. Sulphonamide resistance genes sul2 and sul3 were identified, which is in line with other reports on birds [ 43 , 49 ] and rodents [ 40 ].…”

Section: Discussionsupporting

confidence: 91%

“…Two types of ESBL resistance genes ( bla TEM and bla CTX-M ) were detected in MDR E. coli isolates and both resistance genes encode for Amber Class A Beta-lactamases [ 45 ]. The most commonly detected ESBL resistance gene bla TEM-1B , encodes for TEM-1 Beta-lactamase that hydrolyses Penicillins and First Generation Cephalosporins [ 7 ], and was reported in another study on birds [ 49 ]. The gene bla TEM-176 , was reported in birds such as gull and rook [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Ong

Khor²,

Quek

et al. 2020

IJERPH

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) in Escherichia coli (E. coli) poses a public health concern worldwide. Wild birds and rodents, due to their mobility, are potential vehicles for transmission of AMR bacteria to humans. Ninety-six wild birds’ faecal samples and 135 rodents’ droppings samples were collected and analysed in 2017. Forty-six E. coli isolates from wild birds and rodents were subjected to AMR phenotypic and genotypic characterisation. The proportion of E. coli isolates resistant to at least one of the antimicrobials tested from wild birds (80.8%) was significantly higher than that of isolates from rodents (40.0%). The proportion of E. coli isolates resistant to each antimicrobial class for wild birds was 3.8% to 73.1% and that for rodents was 5.0% to 35.0%. Six out of 26 E. coli isolates from wild birds (23.1%) and two out of 20 (10.0%) isolates from rodents were multi-drug resistant (MDR) strains. These MDR E. coli isolates were detected with various antimicrobial resistance genes such as blaTEM-1B and qnrS1 and could be considered as part of the environmental resistome. Findings in this study suggested that wild birds and rodents could play a role in disseminating antimicrobial resistant E. coli, and this underscores the necessity of environment management and close monitoring on AMR bacteria in wild birds and rodents to prevent spreading of resistant organisms to other wildlife animals and humans.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Ong

Khor²,

Quek

et al. 2020

IJERPH

View full text Add to dashboard Cite

show abstract

“…MDR was identified in 42 out of 59 AMR isolates (71.2%). The prevalence of MDR isolates from small mammals in Hanoi was higher than those observed in Vancouver, Canada birds and rodents were concerned as potential carriers and/ or spreaders of colistin-resistant E. coli [1,2,4,13,34,45,47]. Our data revealed that urban rodents could be a carrier and spreader of colistin-resistant E. coli ( Table 6).…”

Section: Discussionmentioning

confidence: 54%

Antibiotic-resistant Escherichia coli isolated from urban rodents in Hanoi, Vietnam

Huy

Koizumi

Ung

et al. 2020

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is a global public health concern for both clinical and veterinary medicine. Rodent feces are one of the major infectious sources of zoonotic pathogens including AMR bacteria. So far, there are limited studies reported focused on Escherichia coli isolated in rodent feces from rural and suburban areas in Vietnam. In this study, we investigated the prevalence of antimicrobial resistance in E. coli isolated from feces samples of 144 urban rodents caught in Hanoi, Vietnam. A total of 59 AMR E. coli was isolated from urban rodents of which 42 were multidrug-resistant (MDR) isolates (resistance to at least three classes of antimicrobial agents), four were extended-spectrum β-lactamase (ESBL) producing isolates and five were colistin-resistant isolates. The highest prevalence of the resistance was against

show abstract

“…It is worthy of note that the pig-sourced E. coli ST695 did not contain the ESBL CTX-M-15 encoding gene, which is carried by the human-sourced ST695 [36]. However, the pig-sourced E. coli ST695 possessed some other ESBLs encoding genes such as the bla TEM genes (Figure 2), which have also received some attention [38,39].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene

Peng

et al. 2019

Microorganisms

View full text Add to dashboard Cite

Enterobacteriaceae having chromosomally-encoded mcr-1 is rarely reported. In this study, we recovered a chromosomal mcr-1 carrying Escherichia coli, designated HeN100, from the feces of a diarrheal pig in China. Antimicrobial susceptibility testing showed that HeN100 was resistant to three aminoglycosides, twelve β-lactams including three carbapenems, one phenicol, two tetracyclines, two fluoroquinolones, nitrofurantoin, and colistin tested. Oxford Nanopore MinION sequencing revealed that the complete genomes of the multidrug resistant (MDR) HeN100 consisted of a single circular chromosome and five circular plasmids. Bioinformatical analysis determined HeN100 as ST695 and it contained many acquired resistance genes responsible for its MDR phenotypes, including colistin resistance mcr-1 and the carbapenem resistance blaNDM-1, and most of these genes were located on plasmids. However, the mcr-1 was found on the chromosome, and it was located between an IS30-like element ISApl1 and a PAP2-like encoding gene. These three genes consisted of an “ISApl1-mcr-1-orf” segment and inserted in high AT-rich regions. Finally, we found the blaNDM-1 was carried on an IncFII type conjugative plasmid. The conjugation frequency of this plasmid was 7.61 ± 2.11 × 10−5 per recipient, and its conjugation conferred resistance to carbapenems and other β-lactams, as well as aminoglycosides. The spread of this mcr-1/blaNDM-1-carrying E. coli ST695 represents a great concern of public health.

show abstract

First Detection and Genomic Insight into mcr-1 Encoding Plasmid-Mediated Colistin-Resistance Gene in Escherichia coli ST101 Isolated from the Migratory Bird Species Hirundo rustica in Thailand

Cited by 19 publications

References 26 publications

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Antibiotic-resistant <i>Escherichia coli</i> isolated from urban rodents in Hanoi, Vietnam

Characteristics of a Colistin-Resistant Escherichia coli ST695 Harboring the Chromosomally-Encoded mcr-1 Gene

Contact Info

Product

Resources

About