Antibiotic-resistant &lt;i&gt;Escherichia coli&lt;/i&gt; isolated from urban rodents in Hanoi, Vietnam

Huy, Hoang Le; Koizumi, Nobuo; Ung, Trang Thi Hong; Le, Thanh Thi; Nguyen, Hang Le Khanh; Hoang, Phuong Vu Mai; Nguyen, Cam Nhat; Khong, Tuan Minh; Hasebe, Futoshi; Haga, Takeshi; Le, Mai Thi Quynh; Hirayama, Kazuhiro; Miura, Koryo

doi:10.1292/jvms.19-0697

Cited by 18 publications

(18 citation statements)

References 58 publications

(67 reference statements)

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“…Our observation of tet(A) being the most common tetracycline resistance gene in all MDR isolates was in agreement with other studies in birds and rodents [26,39,40]. Isolate C1797 from wild bird was detected with the tet(B) gene-another gene which codes for efflux pump to transport Tetracycline out of bacterial cell [7].…”

Section: Discussionsupporting

confidence: 91%

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Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Ong

Khor²,

Quek

et al. 2020

IJERPH

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Antimicrobial resistance (AMR) in Escherichia coli (E. coli) poses a public health concern worldwide. Wild birds and rodents, due to their mobility, are potential vehicles for transmission of AMR bacteria to humans. Ninety-six wild birds’ faecal samples and 135 rodents’ droppings samples were collected and analysed in 2017. Forty-six E. coli isolates from wild birds and rodents were subjected to AMR phenotypic and genotypic characterisation. The proportion of E. coli isolates resistant to at least one of the antimicrobials tested from wild birds (80.8%) was significantly higher than that of isolates from rodents (40.0%). The proportion of E. coli isolates resistant to each antimicrobial class for wild birds was 3.8% to 73.1% and that for rodents was 5.0% to 35.0%. Six out of 26 E. coli isolates from wild birds (23.1%) and two out of 20 (10.0%) isolates from rodents were multi-drug resistant (MDR) strains. These MDR E. coli isolates were detected with various antimicrobial resistance genes such as blaTEM-1B and qnrS1 and could be considered as part of the environmental resistome. Findings in this study suggested that wild birds and rodents could play a role in disseminating antimicrobial resistant E. coli, and this underscores the necessity of environment management and close monitoring on AMR bacteria in wild birds and rodents to prevent spreading of resistant organisms to other wildlife animals and humans.

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Section: Discussionsupporting

confidence: 91%

“…This gene was found in Enterobacteriaceae from rooks throughout the European continent [55] and human clinical isolates from Korea [56]. Sulphonamide resistance genes sul2 and sul3 were identified, which is in line with other reports on birds [43,49] and rodents [40].…”

Section: Discussionsupporting

confidence: 89%

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Ong

Khor²,

Quek

et al. 2020

IJERPH

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“…). TC resistance was most frequently observed in previous studies, with rates of 78% and 50% observed in E. coli isolated from rodents in Hanoi, Vietnam [ 17 ] and Trinidad and Tobago [ 21 ], respectively. A previous study conducted in 1988 in Java island, Indonesia showed a 13.3% TC resistance in AMR E. coli isolated from rats [ 8 ].…”

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confidence: 99%

“…Isolation and identification of E. coli from fecal samples were performed using Luria-Bertani (LB) broth (Dickinson and Co., Franklin Lakes, NJ, USA), and Deoxycholate-hydrogen sulfide-lactose (DHL) Agar (Eiken Chemical Co., Ltd., Tokyo, Japan). A colony showing typical morphology from each animal was identified to E. coli by the biochemical tests and detecting the yai O gene by PCR, as described in our previous study [ 17 ].…”

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confidence: 99%

“…Next, we examined the resistance genes in the AMR E. coli isolates. The major β -lactamase genes ( bla CTX-M group ( bla CTXM-1 , bla CTX-M-2 , bla CTX-M-8/25 , bla CTX-M-9 ), bla TEM , bla SHV and bla CMY-2 ), sulfonamides ( sul1 , sul2 and sul3 ), quinolone ( qnr A) and tetracycline [ tet (A), tet (B) and tet (C)] were tested by single or multiplex PCR [ 17 ]. The most frequently detected resistance gene was bla TEM (75%: 15/20), followed by tet (A) (70%: 14/20), sul3 (40%: 8/20) and tet (B) (15%: 3/20) ( Table 4…”

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Antimicrobial resistance in <i>Escherichia coli</i> isolated from brown rats and house shrews in markets, Bogor, Indonesia

Huy

Koizumi

Nuradji

et al. 2021

The Journal of Veterinary Medical Science

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The prevalence of antimicrobial resistance (AMR) in small mammals dwelling in the city was used as an indicator of AMR bacteria in the environment. We captured 87 small mammals (79 brown rats and 8 house shrews) in four markets, Bogor, Indonesia in October 2019, and we obtained 20 AMR Escherichia coli ( E. coil ) from 18 brown rats and two house shrews. Of these, eight isolates were determined to be multi-drug resistant (MDR) E. coli , suggesting the potential contamination of AMR E. coli in the markets in Bogor, Indonesia, and that mammals, including humans, are at risk of infection with AMR E. coli from environment.

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Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh

Hossain

Raut

Singh

et al. 2022

Veterinary Medicine & Sci

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Background Babesia and Theileria are potential threats to the livestock industry, causing considerable economic losses. These tick‐borne blood parasites are more prevalent in crossbred cattle than local cattle in Bangladesh. Objectives To confirm the species of Babesia and Theileria in crossbred cattle from the northern part of Bangladesh using conventional and molecular tools. Methods A total of 385 crossbred cattle blood samples were subjected to DNA extraction and PCR. For molecular detection, B. bigemina rhoptry‐associated protein 1a, B. bovis spherical body protein‐4, and Theileria spp. 18S rRNA were used as the marker genes. Results Using PCR, only 72 (18.7%) samples were found piroplasm positive, of which 12.2% Theileria, 4.7% Babesia, and 1.8% mixed infections. Both Babesia (7.3%), Theileria (7.7%) and mixed (2.8%) infections were detected in Sirajganj, and only Theileria (20.4%) was detected in Rangpur district. By PCR and nPCR we detected B. bigemina and T. annulata in Sirajganj district, and Theileria sp. in Rangpur district. The target gene sequences of isolated pathogens confirmed B. bigemina and T. annulata, and Theileria sp from these samples. Blood smears of all samples were also examined microscopically for Babesia and/or Theileria spp. and 14.3% of samples were found positive, of which 5.9% Babesia and 8.3% Theileria. Generally, the pathogens detected in Sirajgang and Rangpur were genetically related to South Asia, particularly South East Asian isolates. Conclusions These findings provide information for a better understanding of the epidemiology of Babesia and Theileria as well as to improve the approaches for diagnosis and control of tick‐borne diseases in Bangladesh.

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Antibiotic-resistant <i>Escherichia coli</i> isolated from urban rodents in Hanoi, Vietnam

Cited by 18 publications

References 58 publications

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Occurrence and Antimicrobial Resistance Traits of Escherichia coli from Wild Birds and Rodents in Singapore

Antimicrobial resistance in <i>Escherichia coli</i> isolated from brown rats and house shrews in markets, Bogor, Indonesia

Molecular detection of Babesia and Theileria from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh

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