First detection and full genome sequence of porcine circovirus type 3 in Russia

Yuzhakov, Anton G.; Raev, S.A.; Alekseev, Konstantin P.; Гребенникова, Т В; Verkhovsky, О.A.; Zaberezhny, Alexei D.; Алипер, Т.И.

doi:10.1007/s11262-018-1582-z

Cited by 65 publications

(49 citation statements)

References 17 publications

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“…PCV3 is a novel circovirus species first discovered in pigs in the USA in 2016 and subsequently in other countries (Chen et al, ; Faccini et al, ; Franzo et al, ; Fux et al, ; Hayashi et al, ; Kedkovid et al, ; Kwon, Yoo, Park, & Lyoo, ; Palinski et al, ; Stadejek, Woźniak, Miłek, & Biernacka, ; Tochetto et al, ; Ye, Berg, Fossum, Wallgren, & Blomström, ; Yuzhakov et al, ). Despite similar genome organization, PCV3 capsid and replicase proteins share only 37.0% identity to PCV2 (Palinski et al, ).…”

Section: Introductionmentioning

confidence: 99%

Does porcine circovirus type 3 (PCV3) interfere with porcine circovirus type 2 (PCV2) vaccine efficacy?

Woźniak

Miłek

Bąska

et al. 2019

Transbound Emerg Dis

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PCV2 is globally spread pathogen involved in a number of diseases (PCVD). Commonly used vaccines against PCV2 are proved to be highly efficacious. The role of recently discovered PCV3 for pig health and interference with PCV2 remains unknown. The study performed on serum samples from seven farms vaccinated against PCV2 and four non-vaccinated showed very low prevalence of PCV2 viremia in the former (3 out of 106 positive serum pools) and high prevalence of PCV2 viremia in the latter (35 out of 60 positive pools). Mean log 10 PCV2 genome equivalents were lower in vaccinated farms (4.8 ± 0.6 log 10 copies/ml) than in non-vaccinated farms (6.3 ± 1.3 log 10 copies/ ml). PCV3 was detected in 31 out of 106 and 12 out of 60 serum pools from vaccinated and non-vaccinated farms, respectively. Mean log 10 PCV3 genome equivalents were significantly (p < 0.05) lower in vaccinated farms (3.9 ± 0.8 log 10 copies/ml) than in non-vaccinated farms (4.4 ± 0.6 log 10 copies/ml). Concurrent PCV2 and PCV3 infection was rare and found only in 1 out of 529 and 4 out of 292 individual serum samples from vaccinated and non-vaccinated farms, respectively. Our results showed lack of impact of PCV3 circulation on PCV2 vaccine efficacy. On the other hand, intensive PCV2 circulation and high viremia detected in non-vaccinated farms did not seem to increase the level of PCV3 infection. K E Y W O R D SPCV2, PCV3, pigs, prevalence, qPCR, viremia

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Section: Introductionmentioning

confidence: 99%

Does porcine circovirus type 3 (PCV3) interfere with porcine circovirus type 2 (PCV2) vaccine efficacy?

Woźniak

Miłek

Bąska

et al. 2019

Transbound Emerg Dis

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“…PCV3 has been reported in major pig-producing countries since the first report in the US in 2016 [6][7][8][9][10][11][12][13][14][15][16][17]. The virus was reported in China in samples taken in 2015 [8] with its possible presence traced back as early as 1996 as revealed in another study [25].…”

Section: Discussionmentioning

confidence: 82%

“…The PCV3 genome is 2000 nucleotides in length with two major inversely arranged ORFs encoding replicase (Rep) and capsid (Cap) proteins [6,7]. So far, the novel pathogen has been reported in major pigproducing countries including China, Poland, South Korea, Italy, Brazil, Germany, Thailand, Denmark, Spain, Sweden and Russia [8][9][10][11][12][13][14][15][16][17]. PCV3 was also identified in pigs with no clinical signs of infection or disease conditions [9][10][11][12][13][14][15][16][17][18] only [9,18] after this sentence.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang

et al. 2019

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Background Porcine circovirus type 3 (PCV3) was first reported in US in 2016. The virus was also identified later in China. Prevalence of PCV3 in Zhejiang province in southeastern China is not clear though it has been reported in many parts of China. Results PCV3 infection and its co-infection with other swine viral pathogens in pig herds of Zhejiang province were retrospectively investigated by quantitative PCR (qPCR) and its sero-prevalence by indirect ELISA. PCV3 was found positive in 67.1% of the 283 clinical samples taken from 2014 to 2017 as shown by qPCR. Single infection with PCV3 accounted for only one-third of the samples, and majority were of co-infections, predominantly with PEDV (41.6%) but generally low with other swine viruses. Indirect ELISA using the PCV3 capsid protein as the coating antigen revealed an average sero-positive rate of 52.6% (40.8 to 60.8%) in 2345 serum samples from 2011 to 2017, with earliest yet high positive findings in samples taken in 2012. Of 203 serum samples, the qPCR method showed more positive findings than ELISA (81.3% vs 56.2%). With 89 serum samples negative by ELISA, vast majority ( n = 81) were found positive by qPCR. There was negative correlation in levels of PCV3 DNA and anti-capsid antibody response. ORF2-based phylogenetic analysis revealed three major groups (PCV3a, PCV3b and PCV3c) of the 200 strains, 38 from this study and 162 reference strains from GenBank. Most of the strains from this study were clustered into PCV3c. Of the putative signature residues of the capsid protein (aa 24, 27, 77 and 150) relative to the three groups, only the PCV3a group strains showed a distinct pattern of residues VKSI (95% of the strains), while the other two groups did not have such a ‘signature’ pattern. Conclusions Results from this study provided further evidence that the novel virus PCV3 was widely distributed in China and might have emerged in Zhejiang province before 2014, most probably back in 2012 when there was high PCV3 sero-prevalence. PCV3 might be viremic in pigs and could spread by fecal shedding. Electronic supplementary material The online version of this article (10.1186/s12917-019-1977-7) contains supplementary material, which is available to authorized users.

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“…) and Russia (Yuzhakov et al . ). In America, there are reports of the presence of PCV3 in the United States (Palinski et al .…”

Section: Introductionmentioning

confidence: 97%

“…PCV3 has been found in asymptomatic pigs (Zheng et al 2017) and in pigs with clinical symptoms similar to those caused by PCV2, such as porcine dermatitis and nephropathy syndrome (PDNS), respiratory disorders and reproductive failures (Palinski et al 2017). PCV3 has been reported in China (Zheng et al 2017), South Korea (Kwon et al 2017), Japan (Hayashi et al 2018) Poland (Stadejek et al 2017), Spain, Denmark, Italy (Franzo et al 2018a,b), Sweden (Ye et al 2018), the United Kingdom (Collins et al 2017) and Russia (Yuzhakov et al 2018). In America, there are reports of the presence of PCV3 in the United States (Palinski et al 2017) and Brazil (Tochetto et al 2018).…”

Section: Introductionmentioning

confidence: 99%

First detection of porcine circovirus type 3 in Colombia and the complete genome sequence demonstrates the circulation of PCV3a1 and PCV3a2

Vargas-Bermúdez

Campos

Bonil

et al. 2019

Veterinary Medicine & Sci

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Porcine circovirus 3 ( PCV 3) was recently discovered and is a new species of the genus circovirus. Clinically, it is associated with absence of symptoms or with different clinical syndromes. It has been reported in different countries of America, Europe and Asia. Last year, in Colombia, some farms have reported symptoms similar to those caused by PCV 2. Samples were taken from two farms located in the centre of the country, and the presence of PCV 3 was determined by PCR in two samples, one from a pool of sera and another from mesenteric lymph node. The strains were fully sequenced (GenBank accession numbers MH 327784 and MH 327785) and classified into subgroups a1 and a2. According to this classification and its analysis, strain a2 is located within the group called “Linker” that may be evolving towards group “b”. In addition to the above, the two Colombian strains were compared with 104 strains reported in the GenBank database. The phylogenetic tree obtained grouped according to the classification of subgroups a1, a2, b1 and b2. It was found that subgroups a1 and a2 were well grouped when comparing whole genomes, but the same was not observed with the strains of group “b”. In the latter, no subgroups were evidenced when comparing complete genomes. It is suggested that a new classification of PCV 3 subgroups should be proposed, based on whole genome sequences. This is the first report of PCV 3 in Colombia and its complete genome sequence.

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First detection and full genome sequence of porcine circovirus type 3 in Russia

Cited by 65 publications

References 17 publications

Does porcine circovirus type 3 (PCV3) interfere with porcine circovirus type 2 (PCV2) vaccine efficacy?

Does porcine circovirus type 3 (PCV3) interfere with porcine circovirus type 2 (PCV2) vaccine efficacy?

Prevalence of porcine circovirus type 3 in pigs in the southeastern Chinese province of Zhejiang

First detection of porcine circovirus type 3 in Colombia and the complete genome sequence demonstrates the circulation of PCV3a1 and PCV3a2

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