First Detection and Characterization of Macrolide-Resistant <i>Mycoplasma pneumoniae</i> from People with Community-Acquired Pneumonia in Iran

Goodarzi, Narjes Noori; Pourmand, Mohammad Reza; Arfaatabar, Maryam; Azimi, Ghasem; Masoorian, Ensieh; Foroushani, Abbas Rahimi; Aminharati, Farzaneh; Heidarzadeh, Siamak; Baghani, Akram

doi:10.1089/mdr.2019.0223

Cited by 9 publications

(7 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shanghai is still the city with the highest resistance rate than other regions in China and other countries that show decreasing macrolide resistance. 20,[29][30][31][32] This difficult resistant situation in Shanghai could be related to the antimicrobial selection pressure. 33,34 The selection was correlated with antimicrobial usage as observed in the US.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility Profiles and Genetic Characteristics of Mycoplasma pneumoniae in Shanghai, China, from 2017 to 2019

Wang¹,

Zhang²,

Yin³

et al. 2022

IDR

View full text Add to dashboard Cite

Objective The current study investigated the recent genetic characteristics and antimicrobial susceptibility profiles of Mycoplasma pneumoniae ( M. pneumoniae ) in Shanghai, becoming a clinical reference for treating M. pneumoniae infection in Shanghai. Methods Clinical strains were isolated from nasopharyngeal aspirates of the pediatric patients in Shanghai from 2017 to 2019. Nine antimicrobial agents of three antimicrobial classes macrolides, fluoroquinolones and tetracyclines, against M. pneumoniae isolates were investigated using the broth microdilution method. The mechanism of macrolide resistance was analyzed by evaluating the sequences of the 23S rRNA gene and the ribosomal protein genes L4 and L22. Molecular genotyping was undergone to classify the P1 subtypes and the multi-locus variable-number tandem-repeat analysis (MLVA) types. Results A total of 72 isolates were resistant to macrolides (MICs > 64 mg/L for erythromycin) based on the A2063G mutation in the 23S rRNA gene. These strains were susceptible to tetracyclines and fluoroquinolones. P1 type 1 (166/182, 91.2%) and MLVA type 4-5-7-2 (165/182, 90.7%) were the dominant subtypes. MLVA type was associated with the P1 subtypes. The distribution of the P1 subtypes and MLVA types did not change over time. The macrolide-resistant rate in P1 type 2 and MLVA type 3-5-6-2 strains were increased during the three-year study. The 5-loci MLVA typing scheme revealed the clonal expansion of MLVA type 3-4-5-7-2 strains which are macrolide-resistant in 2019. Conclusion Macrolide resistance in M. pneumoniae in Shanghai is very high and is evolving among certain subtypes. Cautions should be taken for the possible clonal spreading of macrolide-resistant genotypes within this populated region.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility Profiles and Genetic Characteristics of Mycoplasma pneumoniae in Shanghai, China, from 2017 to 2019

Wang¹,

Zhang²,

Yin³

et al. 2022

IDR

View full text Add to dashboard Cite

show abstract

“…[ 27 ], who reported a high frequency (26.4%) of this agent in their studied population. The second one was done by our own group and found the rate of M. pneumoniae to be 25.2% [ 6 ]. The prevalence of M. pneumoniae varies in different studies of Iran [ 28 ], which may be influenced by seasonal differences, target group, outbreaks and diagnostic methods.…”

Section: Discussionmentioning

confidence: 99%

“…Mycoplasma pneumoniae , Chlamydia pneumoniae and Legionella pneumophila are the most common bacterial agents of atypical CAP [ 4 ]. M. pneumoniae and L. pneumophila account for 15–40% and 2–15% of atypical CAP in Iran respectively [ 5 , 6 ]. M. pneumoniae and C. pneumoniae are mostly associated with infection in the outpatient setting [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Frequency of Mycoplasma pneumoniae, Legionella pneumophila and Chlamydia spp. among patients with atypical pneumonia in Tehran

Goodarzi

Pourmand

Rajabpour

et al. 2020

New Microbes and New Infections

View full text Add to dashboard Cite

Mycoplasma pneumoniae , Legionella pneumophila and Chlamydia pneumoniae are the most common bacterial agents, which account for 15–40%, 2–15% and 5–10% of atypical community-acquired pneumonia (CAP) respectively. These agents are mostly associated with infection in the outpatient setting. The aim of this study was to evaluate the frequency of these pathogens among patients with CAP attending outpatient clinics in Tehran. A cross-sectional study was carried out of 150 patients attending to educational hospitals in Tehran with CAP. M. pneumoniae , L. pneumophila and Chlamydia spp. were detected by PCR assay, targeting the P1 adhesion gene, macrophage infectivity potentiator ( mip ) gene and 16S rRNA gene respectively from throat swabs obtained from each patient. A total of 86 (57.3%) of 150 patients were women; median age was 50 years (interquartile range, 35–65 years). M. pneumoniae , L. pneumophila and Chlamydia spp. were detected in 37 (24.7%), 25 (16.7%) and 11 (7.3%) patients respectively; of these, 66 patients (44%) were infected at least by one of these three pathogens. The frequency of L. pneumophila was significantly higher among patients over 60 years old (p 0.03). Coinfection was detected in seven patients (4.7%); six were infected by M. pneumoniae and L. pneumophila , and only one was infected by L. pneumophila and Chlamydia spp. M. pneumoniae was the most prevalent agent of atypical CAP, and L. pneumophila was more likely to infect elderly rather than younger people. Further studies on the prevalence of CAP and its aetiologic agents are needed to improve the diagnosis and treatment of CAP patients.

show abstract

“…Research by Arfaatabar et al indicated that the frequency of M. pneumoniae among patients with atypical communityacquired pneumonia was reported to be high (4). Study by Noori et al estimated the macrolide-resistant M. pneumoniae incidence to be almost 57% (5). In clinical practice, characteristics of macrolide-resistant M. pneumonia and macrolide-susceptible M. pneumonia are similar (6).…”

mentioning

confidence: 99%

Macrolide-resistant Mycoplasma pneumoniae in adults with community acquired pneumonia: challenges and risks

Hakamifard

Saffaei

2020

East Mediterr Health J

View full text Add to dashboard Cite

Mycoplasma pneumoniae is an important atypical microorganism that causes community-acquired pneumonia (1). These atypical microorganisms are responsible for 7-20% of community-acquired pneumonia cases, yet β-lactam agents are not effective against M. pneumoniae because it lacks a rigid cell wall. Currently, macrolides are considered as first line agents to treat atypical community-acquired pneumonia due to M. pneumoniae, while respiratory fluoroquinolones (such as levofloxacin), and tetracyclines are alternative treatment options (2). However, macrolide-resistant M. pneumoniae is growing globally and the prevalence rate shows variations across countries and regions. Epidemiologic studies have shown that the macrolide-resistant M. pneumoniae rate is between 0% and 15% in the United States of America, while reaching 100% in Asia, with resistance more dominant in low and middle-income countries (3).

show abstract

First Detection and Characterization of Macrolide-Resistant Mycoplasma pneumoniae from People with Community-Acquired Pneumonia in Iran

Cited by 9 publications

References 31 publications

Antimicrobial Susceptibility Profiles and Genetic Characteristics of Mycoplasma pneumoniae in Shanghai, China, from 2017 to 2019

Antimicrobial Susceptibility Profiles and Genetic Characteristics of Mycoplasma pneumoniae in Shanghai, China, from 2017 to 2019

Frequency of Mycoplasma pneumoniae, Legionella pneumophila and Chlamydia spp. among patients with atypical pneumonia in Tehran

Macrolide-resistant Mycoplasma pneumoniae in adults with community acquired pneumonia: challenges and risks

Contact Info

Product

Resources

About