First Description of an Escherichia coli Strain Producing NDM-1 Carbapenemase in Spain

Solé, Mar; Pitart, Cristina; Roca, Ignasi; Fàbrega, Anna; Salvador, Pilar; Muñoz, Laura; Oliveira, Inês; Gascón, Joaquím; Marco, Francesc; Vila, Jordi

doi:10.1128/aac.00642-11

Cited by 86 publications

(52 citation statements)

References 24 publications

(46 reference statements)

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“…BlaOXA-48 carbapenemases belong to Ambler class D. The prevalence of CPE in Spain remains low and is mainly related to VIM-producing isolates (Sánchez-Romero et al 2012). Nevertheless, KPC-2-, KPC-3-, NDM-1-, and OXA-48-producing Enterobacteriaceae were recently detected in Spain (Solé et al 2011). Fecal carriage of CPE isolates has been investigated rarely compared with carriage of isolates producing extended-spectrum β-lactamases (ESBLs) (Tórtola et al 2005), particularly among patients not selected for their relation to outbreak cases.…”

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Detection of carbapenemase-producing Enterobacteriaceae in rectal surveillance cultures in non-hospitalized patients

et al. 2017

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Background: Carbapenem-resistant Enterobacteriaceae have been increasingly reported worldwide since their first identification more than 20 years ago. The early identification of carriers and implementing of cohorting strategies is the only means to prevent nosocomial outbreaks caused by carbapenemase.The aim of this work is to evaluate the prevalence of intestinal colonization with carbapenemase-producing Enterobacteriaceae (CPE) in non-hospitalized patients and assesses a microbiological protocol for screening these isolates in fecal material. Methods: This study was conducted on patients seen at Kasr Alaini hospital and investigated at microbiology laboratory in Cairo University Hospitals, from May 2013 to October 2013. The study was conducted on 600 patients; 450 of them were non hospitalized patients (group I) and came for regular checkup, and 150 were inpatients as control group (group II) from different hospital departments. Results: Based on questionnaire feedback, history and clinical findings, certain patients were suspected to have CPE. Group I includes 337 (56.2%) males and 113 (18.8%) females and group II includes 83 (13.8%) males and 67 (11.2%) females. Age of group I ranged from 18 to 82 years with a mean of 44 years, while the age of group II ranged from 10 to 77 years with a mean of 37 years. Out of 600 fecal samples (450 from group I and 150 from group II) 12 (carriage rate 2%) were positive by disk diffusion methods, Chrom agar-KPC and or Modified Hodge Test (MHT) and PCR. Two of them were non hospitalized patients (group I) (carriage rate 0.33%) and 10 were hospitalized patients (control group) (carriage rate 1. 7%) from different hospital departments. Conclusions: We describe a fecal carriage of carbapenam producing Enterobactericae ,all of them are NAD producers orOXA-48 producers in patients non infected by these organisms.

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Detection of carbapenemase-producing Enterobacteriaceae in rectal surveillance cultures in non-hospitalized patients

et al. 2017

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“…Isolation of K. pneumoniae co-producing NDM-1 and CTX-M-15, as previously described (Poirel et al, 2011;Solé et al, 2011) is a relevant epidemiological issue because CTX-M-15 and NDM-1 may share a common source in the Indian subcontinent. Effective measures to control the spread of NDM-1 and other carbapenemases are strongly needed.…”

Section: Resultsmentioning

confidence: 99%

Abdominal abscess due to NDM-1-producing Klebsiella pneumoniae in Spain

Oteo

Domingo-García

Fernández-Romero

et al. 2012

Journal of Medical Microbiology

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We describe a clinical case of an abdominal abscess due to NDM-1-producing Klebsiella pneumoniae in a 35-year-old Spanish patient after hospitalization in India for perforated appendicitis and peritonitis. The strain belonged to the MLST type 231 and had multiple additional antibiotic resistance genes such as bla CTX-M-15 , armA methylase, aac(69)-Ib-cr, dfrA12, sul1 and qnrB and lack of porin genes ompK35 and ompK36. The patient was cured after abscess drainage. IntroductionAcquired carbapenemases are increasingly reported in Gram-negative pathogens (Miriagou et al., 2010). Metallob-lactamases belong to the class B carbapenemases and confer resistance to all b-lactams, including carbapenems, except aztreonam. Spread of metallo-b-lactamases among members of the Enterobacteriaceae represents a public health threat. Although VIM and KPC types are the most common carbapenemases in Europe, the class D OXA-48 carbapenemase is also spreading, and the New Delhi metallo-blactamase 1 (NDM-1) has been described in Sweden (Yong et al., 2009) We describe a clinical case of NDM-1-producing Klebsiella pneumoniae in Spain causing an abdominal abscess after surgery for perforated appendicitis. To the best of our knowledge, this is the first case of NDM-1-producing K. pneumoniae in our country. Case reportA 35-year-old white male was admitted to a third level teaching hospital in Madrid (Spain) in August of 2010 due to acute abdominal pain in the right iliac fossa. In the previous 9 days, the patient had been hospitalized in India because of acute perforating appendicitis followed by peritonitis. The patient underwent a laparoscopic appendectomy and peritoneal cavity washing. Pseudomonas aeruginosa and Escherichia coli were cultured from the peritoneal fluid, both susceptible to levofloxacin and meropenem, and treatment with one unidentified antibiotic was prescribed for 5 days.The patient returned to Spain, and 2 days after completing antibiotic treatment started to have pain in the right iliac fossa and had a temperature of 38.8 u C. A haemogram revealed leukocytosis and an abdominal echography showed a pus collection in the right iliac fossa. Blood cultures were obtained, and antimicrobial therapy with amoxicillin/ clavulanic acid (1 g t.i.d.) was prescribed. Three days later, leukocytosis continued, treatment was changed to meropenem (1 g t.i.d.) and the abscess was then drained. The purulent exudate was cultured onto conventional media for aerobic and anaerobic bacteria following standard methodologies. Growth was positive for Enterococcus faecium and carbapenem-resistant K. pneumoniae but anaerobic cultures were negative. Blood cultures were also negative.Although the patient was not specifically treated with antibiotics active against carbapenem-resistant K. pneumoniae, his health condition improved over the next 2 weeks, probably due to the pus drainage. A control echography did not reveal any pus collection and the patient was discharged 16 days after pus drainage. No secondary clinical cases were observed. MethodsAn...

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“…areas around the world [11,15,19,20]. In terms of human hosts, there are three major routes to acquire an NDM-1 producing organism: nosocomial, personal travel and community acquisition.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Characteristics of blaNDM-1 in Enterobacteriaceae and Acinetobacter calcoaceticus–Acinetobacter baumannii Complex in China from 2011 to 2012

Ou¹,

Lv²

2014

Adv Pharmacoepidem Drug Safety

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First Description of an Escherichia coli Strain Producing NDM-1 Carbapenemase in Spain

Cited by 86 publications

References 24 publications

Detection of carbapenemase-producing Enterobacteriaceae in rectal surveillance cultures in non-hospitalized patients

Detection of carbapenemase-producing Enterobacteriaceae in rectal surveillance cultures in non-hospitalized patients

Abdominal abscess due to NDM-1-producing Klebsiella pneumoniae in Spain

Epidemiological Characteristics of blaNDM-1 in Enterobacteriaceae and Acinetobacter calcoaceticus–Acinetobacter baumannii Complex in China from 2011 to 2012

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