Background: Carbapenem-resistant Enterobacteriaceae have been increasingly reported worldwide since their first identification more than 20 years ago. The early identification of carriers and implementing of cohorting strategies is the only means to prevent nosocomial outbreaks caused by carbapenemase.The aim of this work is to evaluate the prevalence of intestinal colonization with carbapenemase-producing Enterobacteriaceae (CPE) in non-hospitalized patients and assesses a microbiological protocol for screening these isolates in fecal material. Methods: This study was conducted on patients seen at Kasr Alaini hospital and investigated at microbiology laboratory in Cairo University Hospitals, from May 2013 to October 2013. The study was conducted on 600 patients; 450 of them were non hospitalized patients (group I) and came for regular checkup, and 150 were inpatients as control group (group II) from different hospital departments. Results: Based on questionnaire feedback, history and clinical findings, certain patients were suspected to have CPE. Group I includes 337 (56.2%) males and 113 (18.8%) females and group II includes 83 (13.8%) males and 67 (11.2%) females. Age of group I ranged from 18 to 82 years with a mean of 44 years, while the age of group II ranged from 10 to 77 years with a mean of 37 years. Out of 600 fecal samples (450 from group I and 150 from group II) 12 (carriage rate 2%) were positive by disk diffusion methods, Chrom agar-KPC and or Modified Hodge Test (MHT) and PCR. Two of them were non hospitalized patients (group I) (carriage rate 0.33%) and 10 were hospitalized patients (control group) (carriage rate 1. 7%) from different hospital departments. Conclusions: We describe a fecal carriage of carbapenam producing Enterobactericae ,all of them are NAD producers orOXA-48 producers in patients non infected by these organisms.