First derivative synchronous fluorescence spectroscopy for the determination of Gatifloxacin in presence of its oxidative degradation product: Application to pharmaceutical preparation

Salama, Fathy M.; Attia, Khalid A.M.; Said, Ragab; El-Attar, Abdul-Aziz M.M.

doi:10.1016/j.saa.2018.08.003

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…It acts by blocking bacterial DNA replication through adhesion to DNA gyrase. [13] Several approaches for determining GTF have been reported such as spectrophotometry, [14,15] spectrofluorimetry, [16,17] and HPLC methods. [18][19][20] Micellar mobile phase (MLC) has recently sparked a lot of interest in liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Gammal

El‐Wasseef

El‐Ashry

et al. 2022

J Chinese Chemical Soc

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Multiple sensitive eco-friendly analytical techniques were applied for the assessment of two important fourth-generation fluoroquinolones namely, gemifloxacin (GFX), and gatifloxacin (GTF). GFX, which is utilized as an essential adjunct therapy for the management of pneumonia in SARS-CoV-2 was determined by green indicating micellar liquid chromatographic in authentic powder and dosage forms. The separation was performed by using a reversed-phase C 18 column; the micellar mobile phase comprised of 12.5% npropanol, 0.15 M sodium lauryl sulfate, and 0.3% triethylamine in 0.02 M Ophosphoric acid adjusted to pH 3.0 and pumped at 1 ml/min flow rate and detected at 266 nm by UV detection. The method was linear over the range of 4.0-90 μg.ml À1 with a recovery percentage of 99.81 ± 0.69. The acidic degradation behavior of GFX along with the kinetic investigation was evaluated as recommended by ICH-stress conditions. The separation of the degradation product was performed in good elution time (less than 8 min), and the suggested technique has a good reproducibility (R.S.D. less than 1.0%) and provides an excellent resolution (R s = 4.5) between GFX and its degradation product. Concerning the second drug, GTF, simple, extremely sensitive, and accurate spectrofluorimetric and spectrophotometric techniques were adopted for the determination of the drug in its pharmaceutical preparations. The suggested approaches utilize the concept of complex formation with eosin Y in an aqueous buffered solution. Regarding the spectrophotometric method (method I), the binary complex has a maximum absorption at 544 nm, while for spectrofluorimetric determination (method II) the formed complex was recorded at λ em 491 nm after excitation at 286 nm. For both approaches, variables affecting the formation of the binary complexes were extensively investigated and optimized. The suggested techniques were shown to be rectilinear across the concentration range of 0.5-8 and 0.05-1 μg.ml À1 , respectively, with recovery percentages of 100.34 ± 0.85 and 99.90 ± 0.81 for method (I) and method (II), respectively. In compliance with ICH guidelines, the analytical performance of

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Section: Introductionmentioning

confidence: 99%

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Gammal

El‐Wasseef

El‐Ashry

et al. 2022

J Chinese Chemical Soc

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“…Gatifloxacin (HGAT), Figure 1 is an important member of fluoroquinolone family known as [1-cyclopropyl-6-fluoro-1,4-dihydro-8-methoxy-7-(3-methyl piperazine-1-yl)-4-oxo-3-quinoline carboxylic acid] and has been widely used to treat the infections in human medicine caused by Gram-positive and Gram-negative bacteria [ 1 , 2 ]. Although in reviewing the literature, no official technique has yet been reported for HGAT evaluation in its bulk and pharmaceutical formulation [ 3 , 4 ], there are a lot of analytical methods have been reported by many researchers describe HGAT determination including UV-vis spectrophotometry, atomic absorption spectrophotometry, spectrofluorometry, liquid chromatography, high-performance liquid chromatography, liquid chromatography-mass spectrometry, fluorescence methods, capillary electrophoresis and flow injection analysis [ 5 , 6 , 7 , 8 , 9 , 10 ]. Generally, UV-vis spectrophotometric determination is the most widely used method for drugs determination in their dosage forms due to its simplicity, low cost and wide availability in laboratories [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The use of complex formation manner for spectrophotometric analysis of gatifloxacin drug based on Co(II), Ni(II) and La(III) ions

Mostafa

El–Wahab

Salman

et al. 2021

Heliyon

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Herein, a simple and accurate spectrophotometric method was developed to detect gatifloxacin (HGAT) in a pure and ophthalmic formulation. The method depends on complexation of HGAT with Co (II), Ni (II) and La(III) ions in ethanol medium at room temperature. The experimental conditions have been investigated to reach optimum conditions for HGAT-metal ions interaction, including detection of a suitable wavelength, medium pH, reaction time and reactants concentration. Moreover, the composition of these complexes in addition to their stability constants were also investigated and the result indicated that the molar ratio of HGAT: Metal ion is 1:1 for Ni (II) and La(III) ions and 1:2 for Co (II) ion. Beer's law plots were obeyed in the concentration ranges 18.77–150.16, 18.77–131.39 and 18.77–112.62 (μg mL −1 ) for Co(II), Ni(II) and La(III) ions interaction, respectively. The apparent molar absorptivity, Sandell's sensitivity, standard deviation, detection and quantification limits were calculated. The proposed method was successfully applied for the determination of HGAT in the bulk and ophthalmic formulation. The obtained results were compared statistically with other published methods and the results were in good agreement with those obtained by reported methods.

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Enzyme-free hybridization chain reaction-based signal amplification strategy for the sensitive detection of Staphylococcus aureus

Tang

Wang

Zhou

et al. 2019

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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First derivative synchronous fluorescence spectroscopy for the determination of Gatifloxacin in presence of its oxidative degradation product: Application to pharmaceutical preparation

Cited by 6 publications

References 29 publications

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

The use of complex formation manner for spectrophotometric analysis of gatifloxacin drug based on Co(II), Ni(II) and La(III) ions

Enzyme-free hybridization chain reaction-based signal amplification strategy for the sensitive detection of Staphylococcus aureus

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