First-derivative spectrophotometric and LC determination of cefuroxime and cefadroxil in urine

El‐Gindy, Alaa; Walily, Abdel Fattah M. El; Bedair, Mohamed

doi:10.1016/s0731-7085(00)00306-x

Cited by 49 publications

(29 citation statements)

References 11 publications

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“…DS has the potential to satisfy these criteria. Derivative spectrophotometry has been applied to simultaneous and individual determination of cefuroxime and cefadroxil, cepfrozil in urine, 337,338 coenzyme Q10 in plasma, 339 fleroxacin in serum, 340 metronidazole and miconazole nitrate in ovules, 343 naproxen, pefloxacin, piroxicam and 5-hydroxypiroxicam [344][345][346][347] in plasma and serum and propofol in biological fluids in pregnant women. 348 Analytical characteristics of derivative procedures for clinic and forensic analysis are summarized in Table 5.…”

Section: ·5 Clinical Analysismentioning

confidence: 99%

Recent Developments of Derivative Spectrophotometry and Their Analytical Applications

El‐Sayed

El-Salem

2005

ANAL. SCI.

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The zero-crossing derivative spectra method is the most common procedure for the simultaneous determination of binary mixtures of their overlapping spectra. As an example, the derivative spectra of binary mixtures of phospho-and silicomolybdenum blue are illustrated in Fig. 1. 23 By measuring the derivative values (heights h1 in Fig. 1) for mixtures of phosphate and silicate at 806 nm (zero-crossing wavelength of silicomolybdate complex), one can avoid the interference by silicate and the phosphate concentration can be determined. By measuring the derivative values (heights h2 in Fig. 1 Moreover, one disadvantage of the zero-crossing method is the necessity of selecting critical wavelengths for the measurements. This selection causes considerable loss of sensitivity and precision in case of binary mixtures. The so-called "ratio spectra derivative" permits the use of the wavelengths corresponding to maximum or minimum and also the use of the distance between consecutive maximum and minimum, thus avoiding these problems. 24 Salinas et al. 25 developed a derivative quotient spectra method with a standard divisor when the spectra of the components are overlapped. The method was based on dividing the spectrum for a mixture into the standard spectra for each of the analyses and driving the quotient to obtain a spectrum that is independent of the analyte concentration used as a divisor. The use of standardized spectra as divisors minimizes experimental errors and background noise. 26 An accurate choice of standard divisors and working wavelengths is fundamental for several reasons. 25,27,28 Easy measurements on separate peaks, higher values of the analytical signals and no need to work only at zero-crossing points (sometimes co-existing compounds have no maximum or minimum at these wavelengths) are advantages for ratio spectra derivative spectrophotometry in comparison with the zerocrossing derivative spectrophotometry. Also, the presence of a lot of maxima and minima in ratio spectra derivative data was another advantage, since these wavelengths give an opportunity for the determination of these compounds in the presence of other active compounds and excipients that possibly interfered with the assay. 29 2·1 Resolution of binary and ternary mixtures by applyingderivative ratio spectra (derivative quotient spectra) The absorption spectrum of a mixture of three compounds; A, B and C is determined by this equation:AM,λ i = εA,λ i CA + εB,λ i CB + εC,λ i CC (path-length is 1 cm)where, AM,λ i is the absorbance value of the mixture at wavelength λi, εA,λ i , εB,λ i and εC,λ i are the molar absorptivities of A, B and C at λi, CA, CP and CC are the molar concentrations of A, B and C, respectively. When we divide this spectrum by the spectrum of a standard solution of one of the components (e.g., A of concentration CA). That is bywe obtainThe first derivative of this spectrum ratio givesIf CC = 0, we have the binary mixture of A and B, and the spectrum in this case is:In Eq. (5) we can see that the derivative ratio spectrum of...

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Section: ·5 Clinical Analysismentioning

confidence: 99%

Recent Developments of Derivative Spectrophotometry and Their Analytical Applications

El‐Sayed

El-Salem

2005

ANAL. SCI.

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“…[5,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] Buffers can damage the equipment column and shorten the life of the equipment by interacting the organic salts with the silica. [12,35] In addition, it was not found in the literature the use of ethanol and water only as a mobile phase for the analysis of cefadroxil by HPLC,…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Green Rp-HPLC Method for Quantification of Cefadroxil Capsules

Marco¹

2017

WJPPS

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“…A detailed survey of analytical literature for CEF revealed several methods based on varied techniques, viz, HPLC 11,12,13 , Spectrophotometery 14,15,16 , Spectrofluorimetry 17 and specific stability-indicating method by UV-Visible method 18 . Similarly, a survey of the analytical literature for sul revealed several methods based on varied techniques, viz HPLC 19,20,21,22 , Spectrophotometery 23,24,25 , HPTLC 26 .…”

Section: Introduction: Cefuroxime Sodium Is Sodium(7r)mentioning

confidence: 99%

Untitled

2012

IJPSR

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Cefuroxime is a 2 nd -generation cephalosporin and Sulbactam is a β-Lactamase inhibitor. The combination formulation is used for the treatment of lower respiratory tract infection. Two new, simple, accurate and precise UV spectrophotometric methods have been developed and validated for the simultaneous determination of Cefuroxime Sodium (CEF) and Sulbactam Sodium (SUL) in their combined dosage forms. First method is based on simultaneous estimation of Cefuroxime at 279nm and Sulbactam at 259 nm, while other Q-absorption Ratio method using two wavelengths, 259nm (λmax of SUL) and 272nm (Isoabsorptive point). 0.01 N NaOH was the solvent used in all methods. Cefuroxime Sodium showed linearity in the range of 8-32μg/mL and Sulbactam sodium showed linearity in the range of 4-16μg/mL in all the methods. All methods were validated statistically and recovery studies were carried out. All methods were found to be accurate, precise and reproducible. These methods were applied to the assay of the drugs in marketed formulation, which were found in the range of 98.0% to 100.0% of the labelled value for both Cefuroxime and Sulbactam. Hence, the methods herein described can be successfully applied in quality control of combined pharmaceutical dosage forms.

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First-derivative spectrophotometric and LC determination of cefuroxime and cefadroxil in urine

Cited by 49 publications

References 11 publications

Recent Developments of Derivative Spectrophotometry and Their Analytical Applications

Recent Developments of Derivative Spectrophotometry and Their Analytical Applications

Development and Validation of a Green Rp-HPLC Method for Quantification of Cefadroxil Capsules

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