First demonstration that ascomycetous halophilic fungi (Aspergillus sydowii and Aspergillus destruens) are useful in xenobiotic mycoremediation under high salinity conditions

González-Abradelo, Deborah; Pérez-Llano, Yordanis; Peidro-Guzmán, Heidy; Sánchez-Carbente, María del Rayo; Folch-Mallol, Jorge Luis; Aranda, Elisabet; Vaidyanathan, Vinoth Kumar; Cabana, Hubert; Gunde‐Cimerman, Nina; Batista-García, Ramón Alberto

doi:10.1016/j.biortech.2019.02.002

Cited by 60 publications

(40 citation statements)

References 45 publications

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“…Therefore, many investigations have focused on the microorganisms that have an ability to biodegrade residual hydrocarbons in soil for cleaning up contaminated sites under growth-limiting conditions (Dhawale et al, 1992;Boonchan et al, 2000;Zheng and Obbard, 2002). Previous studies have shown the advantages of fungi over bacteria for the biodegradation of HMW-hydrocarbons in contaminated soils (Aranda et al, 2017;Prenafeta-Boldú et al, 2019): (i) secretion of several low substrate specificity enzymes (e.g., laccases, lignin peroxidases, and Mn peroxidases) (Harms et al, 2011); (ii) osmo-and xerotolerance of several fungal species that confers an ability to grow in rather extreme and fluctuating environments (Worrich et al, 2017;González-Abradelo et al, 2019;Peidro-Guzmán et al, 2020); and (iii) the capacity of filamentous fungi to form mycelial networks that are often hydrophobic and that might cover several hectares of soil, enhancing the access to hydrocarbon contaminants (Wick et al, 2007;Furuno et al, 2012;Bielèik et al, 2019). These abilities are of particular interest in case of the less water soluble HMW-PAHs, which are strongly adsorbed onto the organic matter and are therefore less available for microbial metabolism (Ghosal et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Bioaugmentation of Native Fungi, an Efficient Strategy for the Bioremediation of an Aged Industrially Polluted Soil With Heavy Hydrocarbons

et al. 2021

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The concurrence of structurally complex petroleum-associated contaminants at relatively high concentrations, with diverse climatic conditions and textural soil characteristics, hinders conventional bioremediation processes. Recalcitrant compounds such as high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) and heavy alkanes commonly remain after standard soil bioremediation at concentrations above regulatory limits. The present study assessed the potential of native fungal bioaugmentation as a strategy to promote the bioremediation of an aged industrially polluted soil enriched with heavy hydrocarbon fractions. Microcosms assays were performed by means of biostimulation and bioaugmentation, by inoculating a defined consortium of six potentially hydrocarbonoclastic fungi belonging to the genera Penicillium, Ulocladium, Aspergillus, and Fusarium, which were isolated previously from the polluted soil. The biodegradation performance of fungal bioaugmentation was compared with soil biostimulation (water and nutrient addition) and with untreated soil as a control. Fungal bioaugmentation resulted in a higher biodegradation of total petroleum hydrocarbons (TPH) and of HMW-PAHs than with biostimulation. TPH (C14-C35) decreased by a 39.90 ± 1.99% in bioaugmented microcosms vs. a 24.17 ± 1.31% in biostimulated microcosms. As for the effect of fungal bioaugmentation on HMW-PAHs, the 5-ringed benzo(a)fluoranthene and benzo(a)pyrene were reduced by a 36% and 46%, respectively, while the 6-ringed benzoperylene decreased by a 28%, after 120 days of treatment. Biostimulated microcosm exhibited a significantly lower reduction of 5- and 6-ringed PAHs (8% and 5% respectively). Higher TPH and HMW-PAHs biodegradation levels in bioaugmented microcosms were also associated to a significant decrease in acute ecotoxicity (EC50) by Vibrio fischeri bioluminiscence inhibition assays. Molecular profiling and counting of viable hydrocarbon-degrading bacteria from soil microcosms revealed that fungal bioaugmentation promoted the growth of autochthonous active hydrocarbon-degrading bacteria. The implementation of such an approach to enhance hydrocarbon biodegradation should be considered as a novel bioremediation strategy for the treatment of the most recalcitrant and highly genotoxic hydrocarbons in aged industrially polluted soils.

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Section: Introductionmentioning

confidence: 99%

Bioaugmentation of Native Fungi, an Efficient Strategy for the Bioremediation of an Aged Industrially Polluted Soil With Heavy Hydrocarbons

et al. 2021

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“…This information will be useful for defining culture conditions in future biotechnological applications of A. sydowii. This fungus can grow with lignocellulosic substrates and hydrocarbons as the only carbon sources [29][30][31][32], and produces biotechnologically relevant enzymes [33][34][35][36][37] and secondary metabolites [38][39][40], which attest to its potential use as a tool in biorefineries and in bioremediation approaches.…”

Section: Introductionmentioning

confidence: 99%

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Rodríguez-Pupo

Pérez-Llano

Tinoco-Valencia

et al. 2021

JoF

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Aspergillus sydowii is a moderate halophile fungus extensively studied for its biotechnological potential and halophile responses, which has also been reported as a coral reef pathogen. In a recent publication, the transcriptomic analysis of this fungus, when growing on wheat straw, showed that genes related to cell wall modification and cation transporters were upregulated under hypersaline conditions but not under 0.5 M NaCl, the optimal salinity for growth in this strain. This led us to study osmolyte accumulation as a mechanism to withstand moderate salinity. In this work, we show that A. sydowii accumulates trehalose, arabitol, mannitol, and glycerol with different temporal dynamics, which depend on whether the fungus is exposed to hypo- or hyperosmotic stress. The transcripts coding for enzymes responsible for polyalcohol synthesis were regulated in a stress-dependent manner. Interestingly, A. sydowii contains three homologs (Hog1, Hog2 and MpkC) of the Hog1 MAPK, the master regulator of hyperosmotic stress response in S. cerevisiae and other fungi. We show a differential regulation of these MAPKs under different salinity conditions, including sustained basal Hog1/Hog2 phosphorylation levels in the absence of NaCl or in the presence of 2.0 M NaCl, in contrast to what is observed in S. cerevisiae. These findings indicate that halophilic fungi such as A. sydowii utilize different osmoadaptation mechanisms to hypersaline conditions.

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“…Aspergillus sydowii, first named in 1926 by Charles Thom and Margaret Brooks Church [16], was reported as a pathogen of gorgonian corals [17,18] and found in different habitats where it survives as a soil decomposing saprotroph [19,20]. Meanwhile A. sydowii has been widely studied for its ability to biodegrade agrochemicals and contaminants [21][22][23]. Moreover, some novel secondary metabolites, such as antidiabetic and antiinflammatory sesquiterpenoids [24], sesquiterpene and xanthone [25], 2-hydroxy-6-formyl-vertixanthone, 12-Oacetyl-sydowinin A [26], and indole alkaloids [27], were found and identified in A. sydowii.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Zhang

Zeng

et al. 2020

BMC Genomics

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Background: Anthocyanins are common substances with many agro-food industrial applications. However, anthocyanins are generally considered to be found only in natural plants. Our previous study isolated and purified the fungus Aspergillus sydowii H-1, which can produce purple pigments during fermentation. To understand the characteristics of this strain, a transcriptomic and metabolomic comparative analysis was performed with A. sydowii H-1 from the second and eighth days of fermentation, which confer different pigment production. Results: We found five anthocyanins with remarkably different production in A. sydowii H-1 on the eighth day of fermentation compared to the second day of fermentation. LC-MS/MS combined with other characteristics of anthocyanins suggested that the purple pigment contained anthocyanins. A total of 28 transcripts related to the anthocyanin biosynthesis pathway was identified in A. sydowii H-1, and almost all of the identified genes displayed high correlations with the metabolome. Among them, the chalcone synthase gene (CHS) and cinnamate-4hydroxylase gene (C4H) were only found using the de novo assembly method. Interestingly, the best hits of these two genes belonged to plant species. Finally, we also identified 530 lncRNAs in our datasets, and among them, three lncRNAs targeted the genes related to anthocyanin biosynthesis via cis-regulation, which provided clues for understanding the underlying mechanism of anthocyanin production in fungi. Conclusion: We first reported that anthocyanin can be produced in fungus, A. sydowii H-1. Totally, 31 candidate transcripts were identified involved in anthocyanin biosynthesis, in which CHS and C4H, known as the key genes in anthocyanin biosynthesis, were only found in strain H1, which indicated that these two genes may contribute to anthocyanins producing in H-1. This discovery expanded our knowledges of the biosynthesis of anthocyanins and provided a direction for the production of anthocyanin.

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First demonstration that ascomycetous halophilic fungi (Aspergillus sydowii and Aspergillus destruens) are useful in xenobiotic mycoremediation under high salinity conditions

Cited by 60 publications

References 45 publications

Bioaugmentation of Native Fungi, an Efficient Strategy for the Bioremediation of an Aged Industrially Polluted Soil With Heavy Hydrocarbons

Bioaugmentation of Native Fungi, an Efficient Strategy for the Bioremediation of an Aged Industrially Polluted Soil With Heavy Hydrocarbons

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

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