First Complete Genome Sequences of Xanthomonas citri pv. vignicola Strains CFBP7111, CFBP7112, and CFBP7113 Obtained Using Long-Read Technology

Ruh, Mylène; Briand, Martial; Bonneau, Sophie; Jacques, Marie‐Agnès; Chen, Nicolas W.G.

doi:10.1128/genomea.00813-17

Cited by 7 publications

(6 citation statements)

References 14 publications

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“…Moreover, recent whole-genome sequencing data indicate that the TALE-encoding genes from X. citri pv. vignicola are very different from those found in Xcf and Xpp (Ruh et al, 2017b; unpublished data). In particular, Xfutal1 is absent in vignicola strains while it is shared by all Xcf-Xpp strains sequenced so far (Ruh et al, 2017a), indicating differential adaptation of these pathovars to either common bean or cowpea.…”

Section: Hos T R Ang Econtrasting

confidence: 57%

“…vignicola (a pathogen of cowpea), together with the fact that both are pathogenic on common bean and cowpea, challenges the boundaries between these two pathovars. A finer description of the symptoms naturally induced by each pathovar on each host could bring essential elements to enlighten (Ruh et al, 2017b; unpublished data). In particular, Xfutal1 is absent in vignicola strains while it is shared by all Xcf-Xpp strains sequenced so far (Ruh et al, 2017a), indicating differential adaptation of these pathovars to either common bean or cowpea.…”

Section: Hos T R Ang Ementioning

confidence: 99%

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Common bacterial blight of bean: a model of seed transmission and pathological convergence

Chen

Ruh

Darrasse

et al. 2021

Molecular Plant Pathology

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Section: Hos T R Ang Econtrasting

confidence: 57%

Section: Hos T R Ang Ementioning

confidence: 99%

Common bacterial blight of bean: a model of seed transmission and pathological convergence

Chen

Ruh

Darrasse

et al. 2021

Molecular Plant Pathology

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“…vesicatoria ) PRJNA341901 (Thieme et al, 2005); X. citri pv. vignicola CFBP7111, PRJNA390891 (Ruh et al, 2017a); X. axonopodis pv. citri 306, PRJNA297 (da Silva et al, 2002); X. campestris pv.…”

Section: Resultsunclassified

Tal1NXtc01 in Xanthomonas translucens pv. cerealis Contributes to Virulence in Bacterial Leaf Streak of Wheat

Shah

Haq

et al. 2019

Front. Microbiol.

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Xanthomonas translucens pv. cerealis (Xtc) causes bacterial leaf streak (BLS) of important cereal crops, including wheat (Triticum aestivum) and barley (Hordeum vulgare). Transcription activator-like effectors (TALEs) play vital roles in many plant diseases caused by Xanthomonas spp., however, TALEs have not been previously characterized in Xtc. In this study, the whole genome of NXtc01, a virulent strain of Xtc from Xinjiang, China, was sequenced and compared with genomes of other Xanthomonas spp. Xtc NXtc01 consists of a single 4,622,298 bp chromosome that encodes 4,004 genes. Alignment of the NXtc01 sequence with the draft genome of Xtc strain CFBP 2541 (United States) revealed a single giant inversion and differences in the location of two tal genes, which were designated tal1 and tal2. In NXtc01, both tal genes are located on the chromosome, whereas tal2 is plasmid-encoded in CFBP 2541. The repeat variable diresidues (RVDs) at the 12th and 13th sites within Tal2 repeat units were identical in both strains, whereas Tal1 showed differences in the third RVD. Xtc NXtc01 and CFBP 2541 encoded 35 and 33 non-TALE type III effectors (T3Es), respectively. tal1, tal2, and tal-free deletion mutants of Xtc NXtc01 were constructed and evaluated for virulence. The tal1 and tal-free deletion mutants were impaired with respect to symptom development and growth in wheat, suggesting that tal1 is a virulence factor in NXtc01. This was confirmed in gain-of-function experiments that showed the introduction of tal1, but not tal2, restored virulence to the tal-free mutant. Furthermore, we generated a hrcC deletion mutant of NXtc01; the hrcC mutant was non-pathogenic on wheat and unable to elicit a hypersensitive response in the non-host Nicotiana benthamiana. Our data provide a platform for exploring the roles of both TALEs and non-TALEs in promoting BLS on wheat.

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“…A similar XopJ family effector (98% amino acid identity) in X. citri pv. vignicola strain CFBP 7112 ( Ruh et al, 2017 ) is also located between phage associated genes. Effector avrBsT is another XopJ family effector found in X. perforans that is generally associated with plasmids ( Ciesiolka et al, 1999 ; Kay and Bonas, 2009 ; White et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Multiple Recombination Events Drive the Current Genetic Structure of Xanthomonas perforans in Florida

et al. 2019

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Prior to the identification of Xanthomonas perforans associated with bacterial spot of tomato in 1991, X. euvesicatoria was the only known species in Florida. Currently, X. perforans is the Xanthomonas sp. associated with tomato in Florida. Changes in pathogenic race and sequence alleles over time signify shifts in the dominant X. perforans genotype in Florida. We previously reported recombination of X. perforans strains with closely related Xanthomonas species as a potential driving factor for X. perforans evolution. However, the extent of recombination across the X. perforans genomes was unknown. We used a core genome multilocus sequence analysis approach to identify conserved genes and evaluated recombination-associated evolution of these genes in X. perforans. A total of 1,356 genes were determined to be “core” genes conserved among the 58 X. perforans genomes used in the study. Our approach identified three genetic groups of X. perforans in Florida based on the principal component analysis (PCA) using core genes. Nucleotide variation in 241 genes defined these groups, that are referred as Phylogenetic-group Defining (PgD) genes. Furthermore, alleles of many of these PgD genes showed 100% sequence identity with X. euvesicatoria, suggesting that variation likely has been introduced by recombination at multiple locations throughout the bacterial chromosome. Site-specific recombinase genes along with plasmid mobilization and phage associated genes were observed at different frequencies in the three phylogenetic groups and were associated with clusters of recombinant genes. Our analysis of core genes revealed the extent, source, and mechanisms of recombination events that shaped the current population and genomic structure of X. perforans in Florida.

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First Complete Genome Sequences of Xanthomonas citri pv. vignicola Strains CFBP7111, CFBP7112, and CFBP7113 Obtained Using Long-Read Technology

Cited by 7 publications

References 14 publications

Common bacterial blight of bean: a model of seed transmission and pathological convergence

Common bacterial blight of bean: a model of seed transmission and pathological convergence

Tal1NXtc01 in Xanthomonas translucens pv. cerealis Contributes to Virulence in Bacterial Leaf Streak of Wheat

Multiple Recombination Events Drive the Current Genetic Structure of Xanthomonas perforans in Florida

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