First Case Report of Bloodstream Infection Due to a Candida Species Closely Related to the Novel Species Candida pseudorugosa

Taverna, Constanza Giselle; Córdoba, Susana; Isla, Guillermina; Fernández, Norma; García, Susana; Mazza, Mariana; Murisengo, Omar Alejandro; Vivot, Walter; Szusz, Wanda; Davel, Graciela; Tiraboschi, Iris Nora; Bosco-Borgeat, María Eugenia

doi:10.1128/jcm.00167-12

Cited by 6 publications

(5 citation statements)

References 42 publications

(47 reference statements)

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“…The species identification was verified with sequencing analysis of the 26S D1/D2 region, and the identity and coverage between the two environmental isolates and the C. pseudorugosa type strain sequences were 99% and 100%, respectively. Finally, Taverna et al (2012) recently described the isolation of specimens from blood and central venous catheters of a female patient in Argentina that were closely related to C. pseudorugosa (Taverna et al, 2012). In our analysis ( Fig.…”

Section: Resultsmentioning

confidence: 63%

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Systematic review and new insights into the molecular characterization of the Candida rugosa species complex

Padovan

Melo

Colombo

2013

Fungal Genetics and Biology

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Recently, Candida rugosa was characterized as a species complex comprising four taxa: C. rugosa sensu stricto, Candida pseudorugosa, Candida neorugosa and Candida mesorugosa. Although considered relatively rare, several clusters of candidemia due to C. rugosa complex had been reported presenting mortality rates close to 70%. In this work we discuss the systematization, phenotyping and molecular methods based on internal transcribed spacer region (ITS) sequencing and proteomic analyses for species identification, as well as clinical aspects of the C. rugosa complex. We performed a Bayesian phylogenetic analysis using 72 ITS sequences representative of C. rugosa complex isolates and related species within the genus. Biochemical, morphological and MALDI-TOF MS analyses were processed with C. rugosa complex type strains and related species isolates. We described that the phylogeny showed four distinct clades inferred with high posterior probabilities, corresponding to the four species within the C. rugosa complex, excluding C. pararugosa. Biochemical and morphological aspects distinguished only C. rugosa sensu stricto but were not sufficient to accurately identify species within the rest of the complex. Protein spectrum profiles differentiated all reference strains from different species analyzed. To our knowledge, we presented the first phylogenetic analysis using a large collection of ITS sequences as well as proteomic profiles generated from isolates of the C. rugosa complex and related species that can enlighten systematics, diagnostics and clinical research fields.

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Section: Resultsmentioning

confidence: 63%

“…The novel species within the C. rugosa complex have been identified based on 26S D1/D2 rDNA sequences, complemented with ITS sequences (Butinar et al, 2011;Li et al, 2006;Paredes et al, 2012;Taverna et al, 2012;Tay et al, 2011). Of note, only C. mesorugosa was characterized based on sequences of rDNA regions as well as housekeeping genes .…”

Section: Resultsmentioning

confidence: 99%

Systematic review and new insights into the molecular characterization of the Candida rugosa species complex

Padovan

Melo

Colombo

2013

Fungal Genetics and Biology

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“…When tested using the CLSI broth microdilution method, all classes of antifungal drugs appeared to show good activity against these four species [17]. A case of bloodstream infection with a novel unnamed species closely related to C. pseudorugosa has also been recently described [18]. This organism was reported as resistant to fluconazole and to some echinocandin drugs when tested according to the EUCAST microdilution protocol [18].…”

Section: Other Non-albicans Candida Speciesmentioning

confidence: 99%

“…A case of bloodstream infection with a novel unnamed species closely related to C. pseudorugosa has also been recently described [18]. This organism was reported as resistant to fluconazole and to some echinocandin drugs when tested according to the EUCAST microdilution protocol [18].…”

Section: Other Non-albicans Candida Speciesmentioning

confidence: 99%

Recent Taxonomic Developments with Candida and Other Opportunistic Yeasts

Brandt

Lockhart

2012

Curr Fungal Infect Rep

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Increases in susceptible patient populations and advances in identification methods have resulted in the continued recognition of novel yeasts as agents of human infection. Most of these agents are members of the well-recognized genera Candida, Cryptococcus, Trichosporon, and Rhodotorula. Some of these agents are "cryptic species," members of species complexes, and may not be detectable using classical carbohydrate assimilation-based methods of yeast identification. Such species require DNA-or MALDI-based methods for correct identification, although sporadic isolates may not routinely require delineation to the individual species level. The coming end of the fungal taxonomy rules requiring separate names for sexual and asexual forms of the same fungus will hopefully allow greater clarity, as names for medically important yeast can now be based on the needs of the medical mycology community and the common goal of better communication between laboratory and clinician.

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“…En todos los ámbitos es destacable que las llamadas especies no-albicans han aumentado globalmente respecto al pasado [21][22][23][24][25], cuando debido al menor número de trasplantes, procedimientos invasivos, colocación de dispositivos protésicos, pacientes infectados con el virus de la inmunodeficiencia humana (VIH), padecimientos oncológicos, dismetabolias, migraciones humanas, e incluso, a las limitaciones técnicas que existían en los laboratorios de microbiología clínica para la caracterización fenotípica de levaduras, la epidemiología proponía a Candida albicans como la especie de mayor importancia médica [26,27]. En la actualidad, se ha propuesto también que el uso temprano o profiláctico de antifúngicos de la familia de los azoles, específicamente fluconazol, contribuye al cambio en el patrón epidemiológico, favoreciendo el aumento de las especies no-albicans [15].…”

Section: Discussionunclassified

Epidemiología de la candidemia y sensibilidad in vitro frente a azoles, anfotericina B y caspofungina en una institución de salud de Valledupar, Colombia

et al. 2015

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Introducción: la candidemia es una enfermedad grave, con elevada morbi-mortalidad y cuyo tratamiento no siempre conduce a la cura. La distribución de especies de Candida y la sensibilidad antifúngica varía según el área geográfica, incluso entre centros de salud de una misma región. Objetivo: establecer la distribución de especies de Candida y su sensibilidad in vitro frente a diferentes antifúngicos. Materiales y métodos: se realizó un estudio descriptivo, prospectivo y transversal entre noviembre de 2013 y mayo de 2014 de casos de candidemias en una institución de salud de Valledupar, Colombia. Las Concentraciones Inhibitorias Mínimas (CIM) se determinaron según el protocolo estándar M27-A3-S4. Resultados: Se estudiaron 40 aislados clínicos de Candida spp. obtenidos de sangre (97,5%) y médula ósea (2,5%). Del total, 15 (37,5%) fueron caracterizados como Candida tropicalis, 13 (32,5%) del Complejo Candida albicans, cinco (12,5%) del Complejo Candida glabrata, tres (7,5%) como Candida guilliermondii, tres (7,5%) del Complejo Candida parapsilosis y uno (2,5%) como Candida krusei. No se observó resistencia a la anfotericina B ni al voriconazol en ninguno de los aislados, pero sí al fluconazol en uno (6,6%) de Candida tropicalis y uno (33,3%) del Complejo Candida parapsilosis y a la caspofungina en el aislado de Candida krusei y en uno (20%) del Complejo Candida glabrata. Conclusiones: la epidemiología local de las levaduras causantes de candidemia mostró mayor prevalencia de especies no-albicans, entre las que se encontró resistencia a los antifúngicos evaluados, lo que es relevante para la elección e instauración de un tratamiento eficaz.

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First Case Report of Bloodstream Infection Due to a Candida Species Closely Related to the Novel Species Candida pseudorugosa

Cited by 6 publications

References 42 publications

Systematic review and new insights into the molecular characterization of the Candida rugosa species complex

Systematic review and new insights into the molecular characterization of the Candida rugosa species complex

Recent Taxonomic Developments with Candida and Other Opportunistic Yeasts

Epidemiología de la candidemia y sensibilidad in vitro frente a azoles, anfotericina B y caspofungina en una institución de salud de Valledupar, Colombia

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