First- and second-derivative spectrophotometric determination of imipenem and cilastatin in injections

Parra, Alberto; Garcia-Villanova, J; Ródenas, V.; Gómez, M. A.

doi:10.1016/0731-7085(93)80160-3

Cited by 6 publications

(1 citation statement)

References 17 publications

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“…A review of literature shows many analytical studies applied to imipenem in biological fluids, involving HPLC-DAD (6)(7)(8), and spectrophotometric/chemometric technique (9). On the other hand, for pharmaceutical dosage forms, the literature reports derivate spectrophotometric (10,11), and RP-UPLC method for quantitation of imipenem related impurities (12). In fact, antibiotics play an increasingly important role in pharmacotherapy because of their wide use in the clinical practice, so it is extremely important to develop alternative methods that allow their analytical determination in existing pharmaceutical forms (13).…”

Section: Introductionmentioning

confidence: 99%

Microbiological Assay for Quantitative Determination of Imipenem in Powder for Injection

et al. 2018

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This work describes the development and validation of a microbiological method using the cylinder-plate assay for quantitative determination of imipenem in powder for injection. The aim was to obtain a low-cost and suitable methodology that can be alternative to physicochemical techniques already described, contributing for the quality control of this antibiotic. Firstly, the analytical conditions were optimized, testing the microorganism, inoculum concentration and best range of sample and standard concentrations, in a way that provides the adequate measurement of the inhibition halos. Staphylococcus epidermidis ATCC 12228 was selected as test microorganism, as well as 2.0 % of inoculum concentration. The validation protocol followed the official guidelines, and the parameters evaluated were linearity, precision (intermediate precision and repeatability) and accuracy. All standard curves ranging 0.5-2.0 µg/mL showed r values higher than 0.999, and ANOVA confirmed that were no deviation from linearity (p-value > 0.05). The method also proved to be precise with RSD (relative standard deviation) values ranging 0.28-0.64 for repeatability and 2.49 for intermediate precision. It was performed three days of experiments, being three assays of eight plates a day. The drug mean content was 101.05%. Accuracy was assessed by recovery test, with standard recovery percentage of 101.70-107.90% (mean recovery = 104.86%), which was considered satisfactory. Therefore, the proposed microbiological method was considered validated and suitable for application in quantitative determination of this drug, being useful for quality control routine.

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Section: Introductionmentioning

confidence: 99%

Microbiological Assay for Quantitative Determination of Imipenem in Powder for Injection

et al. 2018

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Determination of a ternary mixture of penicillin-G sodium salt, penicillin-G procain salt and dihydrostreptomycin sulphate, by third-derivative spectrophotometry

Morelli¹

1994

Talanta

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First-order derivative ultraviolet spectrophotometry of imipenem–cilastatin formulations

Tabbouche

2014

Journal of Taibah University Medical Sciences

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First- and second-derivative spectrophotometric determination of imipenem and cilastatin in injections

Cited by 6 publications

References 17 publications

Microbiological Assay for Quantitative Determination of Imipenem in Powder for Injection

Microbiological Assay for Quantitative Determination of Imipenem in Powder for Injection

Determination of a ternary mixture of penicillin-G sodium salt, penicillin-G procain salt and dihydrostreptomycin sulphate, by third-derivative spectrophotometry

First-order derivative ultraviolet spectrophotometry of imipenem–cilastatin formulations

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