Firefly luciferase inhibition

Leitão, João M.M.; Silva, Joaquim C. G. Esteves da

doi:10.1016/j.jphotobiol.2010.06.015

Cited by 93 publications

(64 citation statements)

References 53 publications

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“…There are also important caveats to the quantitation of ATP by luciferase. For example, optimization is critical because of the inherent dependence on oxygen concentration and the often underappreciated complication of product inhibition as well as inhibition by cellular factors or pharmacological agents (Leitão and Esteves da Silva, 2010; Lundin, 2014). …”

Section: Methods For Detection and Imaging Atpmentioning

confidence: 99%

Imaging Adenosine Triphosphate (ATP)

Rajendran

Dane

Conley

et al. 2016

The Biological Bulletin

105

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Adenosine triphosphate (ATP) is a universal mediator of metabolism and signaling across unicellular and multicellular species. There is a fundamental interdependence between the dynamics of ATP and the physiology that occurs inside and outside the cell. Characterizing and understanding ATP dynamics provides valuable mechanistic insight into processes that range from neurotransmission to the chemotaxis of immune cells. Therefore, we require the methodology to interrogate both temporal and spatial components of ATP dynamics from the subcellular to organismal levels in live specimens. Over the last several decades, a number of molecular probes that are specific for ATP have been developed. These probes have been combined with imaging approaches, particularly optical microscopy, to enable qualitative and quantitative detection of this critical molecule. In this review, we survey current examples of technologies that are available to visualize ATP in living cells and identify areas where new tools and approaches are needed to expand our capabilities.

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Section: Methods For Detection and Imaging Atpmentioning

confidence: 99%

Imaging Adenosine Triphosphate (ATP)

Rajendran

Dane

Conley

et al. 2016

The Biological Bulletin

105

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show abstract

“…There are many studies about the LUC inhibitors and corresponding inhibition mechanisms (Leitao and Esteves da Silva, 2010;Vieira et al, 2012). Ribeiro and Esteves da Silva (2008) reveals that oxyluciferin is a competitive inhibitor while dehydroluciferyl-adenylate (L-AMP) acts as a tight-binding competitive inhibitor with respect to LH 2 .…”

Section: Bound To Active Site Of Phosphate Of Amp Results In Stimulationmentioning

confidence: 99%

Blocking the entrance of AMP pocket results in hormetic stimulation of imidazolium-based ionic liquids to firefly luciferase

Liu

et al. 2015

Chemosphere

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“…The reporter enzyme firefly luciferase catalyzes the ATP-dependent conversion of luciferin to oxyluciferin, which is linked to light emission, and firefly luciferase-dependent luminescence is measured as a surrogate for transcription factor activity. Various chemical entities interfere with the catalytic activity of the firefly luciferase enzyme [1]. Such substances might distort and invalidate the results of reporter analyses [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%