Finite element simulations of hydrodynamic trapping in microfluidic particle-trap array systems

Xu, Xiaojun; Li, Zhenyu; Nehorai, Arye

doi:10.1063/1.4822030

Cited by 38 publications

(29 citation statements)

References 31 publications

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“…In the design of our cell trapping system, we used computational fluid dynamics simulations coupled with solid mechanics, implemented by the Finite Element Method (FEM)-based Comsol Multiphysics. Building the model, we adapted the previous work by Xu et al 26 The simulation results allowed us to predict the time dependent cell motion before trapping occurs, the flow velocity/flux distribution in the structured microfluidic channel, and the mechanical impact of the interaction of the cell with the fluid both before and after being trapped. Fig.…”

Section: A Simulation Resultsmentioning

confidence: 99%

Highly efficient and gentle trapping of single cells in large microfluidic arrays for time-lapse experiments

et al. 2016

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The isolation of single biological cells and their further cultivation in dedicated arrayed chambers are key to the collection of statistically reliable temporal data in cell-based biological experiments. In this work, we present a hydrodynamic single cell trapping and culturing platform that facilitates cell observation and experimentation using standard bio-lab equipment. The proposed design leverages the stochastic position of the cells as they flow into the structured microfluidic channels, where hundreds of single cells are then arrayed in nanoliter chambers for simultaneous cell specific data collection. Numerical simulation tools are used to devise and implement a hydrodynamic cell trapping mechanism that is minimally detrimental to the cell cycle and retains high overall trapping efficiency (∼70%) with the capability of reaching high fill factors (>90%) in short loading times (1-4 min) in a 400-trap device. A Monte Carlo model is developed using the design parameters to estimate the system trapping efficiencies, which show strong agreement with the experimentally acquired data. As proof of concept, arrayed mammalian tissue cells (MIA PaCa-2) are cultured in the microfluidic chambers for two days without viability problems.

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Section: A Simulation Resultsmentioning

confidence: 99%

Highly efficient and gentle trapping of single cells in large microfluidic arrays for time-lapse experiments

et al. 2016

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“…31 One can refer to it for more details to build the finite element simulation model. The accuracy of the 2-D time-dependent simulations of the hydrodynamic trapping of microspheres has been validated by experiments in our previous publication.…”

Section: Finite Element Fluidic Dynamics Simulationsmentioning

confidence: 99%

Simultaneous detection of multiple biological targets using optimized microfluidic microsphere-trap arrays

Sarder

et al. 2014

J. Micro/Nanolith. MEMS MOEMS

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We propose an analytical framework to build a microfluidic microsphere-trap array device that enables simultaneous, efficient, and accurate screening of multiple biological targets in a single microfluidic channel. By optimizing the traps' geometric parameters, the trap arrays in the channel of the device can immobilize microspheres of different sizes at different regions, obeying hydrodynamically engineered trapping mechanism. Different biomolecules can be captured by the ligands on the surfaces of microspheres of different sizes. They are thus detected according to the microspheres' positions (position encoding), which simplifies screening and avoids target identification errors. To demonstrate the proposition, we build a device for simultaneous detection of two target types by trapping microspheres of two sizes. We evaluate the device performance using finite element fluidic dynamics simulations and microsphere-trapping experiments. These results validate that the device efficiently achieves position encoding of the two-sized microspheres with few fluidic errors, providing the promise to utilize our framework to build devices for simultaneous detection of more targets. We also envision utilizing the device to separate, sort, or enumerate cells, such as circulating tumor cells and blood cells, based on cell size and deformability. Therefore, the device is promising to become a cost-effective and point-of-care miniaturized disease diagnostic tool.

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“…Thus, the combination of the bead bioassay with the microfluidic technology can overcome the limitations of the conventional bead based bioassay, while maximizing the merits of the bead chemistry. [6][7][8][9][10] To this end, Tan et al proposed an integrated microdevice for trap-and-release of single microbeads. They used hydrodynamic resistance between the microchannel and the microtrapping site for single bead a) Author to whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

“…16 Although the previous microbead array formats are adequate for sensitive biochemical analysis in a high-throughput manner without cross-contamination between beads, most of their designs were restricted to microwell structure. [8][9][10][11][12][13][14][15][16][17][18] Such a microwell structure mainly depends on the passive immobilization of the beads into each microwell, which is time-consuming, and suffers from the bead aggregate formation, leading to the non-uniform distribution of single beads in each chamber. In addition, the recovery of the immobilized beads is very difficult using the microwell array.…”

Section: Introductionmentioning

confidence: 99%

A large-area hemispherical perforated bead microarray for monitoring bead based aptamer and target protein interaction

et al. 2014

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Herein, we present a large-area 3D hemispherical perforated microwell structure for a bead based bioassay. Such a unique microstructure enables us to perform the rapid and stable localization of the beads at the single bead level and the facile manipulation of the bead capture and retrieval with high speed and efficiency. The fabrication process mainly consisted of three steps: the convex micropatterned nickel (Ni) mold production from the concave micropatterned silicon (Si) wafer, hot embossing on the polymer matrix to generate the concave micropattened acrylate sheet, and reactive ion etching to make the bottom holes. The large-area hemispherical perforated micropatterned acrylate sheet was sandwiched between two polydimethylsiloxane (PDMS) microchannel layers. The bead solution was injected and recovered in the top PDMS microchannel, while the bottom PDMS microchannel was connected with control lines to exert the hydrodynamic force in order to alter the flow direction of the bead solution for the bead capture and release operation. The streptavidin-coated microbead capture was achieved with almost 100% yield within 1 min, and all the beads were retrieved in 10 s. Lysozyme or thrombin binding aptamer labelled microbeads were trapped on the proposed bead microarray, and the in situ fluorescence signal of the bead array was monitored after aptamer-target protein interaction. The protein-aptamer conjugated microbeads were recovered, and the aptamer was isolated for matrix assisted laser desorption/ionization time-of-flight mass spectrometry analysis to confirm the identity of the aptamer.

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Finite element simulations of hydrodynamic trapping in microfluidic particle-trap array systems

Cited by 38 publications

References 31 publications

Highly efficient and gentle trapping of single cells in large microfluidic arrays for time-lapse experiments

Highly efficient and gentle trapping of single cells in large microfluidic arrays for time-lapse experiments

Simultaneous detection of multiple biological targets using optimized microfluidic microsphere-trap arrays

A large-area hemispherical perforated bead microarray for monitoring bead based aptamer and target protein interaction

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