Fingerprinting analysis of fresh ginseng roots of different ages using1H-NMR spectroscopy and principal components analysis

Shin, Yoo-Soo; Bang, Kyong-Hwan; In, Dong-Soo; Kim, Ok-Tae; Hyun, Dong-Yoon; Ahn, In-Ok; Ku, Bon Cho; Kim, Sukweon; Seong, Nak-Sul; Cha, Seon‐Woo; Lee, Dong‐Ho; Choi, Hyung‐Kyoon

doi:10.1007/bf02977333

Cited by 44 publications

(26 citation statements)

References 15 publications

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“…Calcium oxalate and fatty acids in ginseng root also can vary depending on the cultivation area or method [33]. It has been also shown that quantitative changes in aromatic compounds can be used to discriminate ginseng roots with different ages [28]. In the case of the olive tree, secondary metabolites in leaves play a significant role in cultivar discrimination by multivariate analysis [53].…”

Section: Discussionmentioning

confidence: 99%

Discrimination of cultivation ages and cultivars of ginseng leaves using Fourier transform infrared spectroscopy combined with multivariate analysis

Kwon¹,

Ahn²,

Park³

et al. 2014

Journal of Ginseng Research

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To determine whether Fourier transform (FT)-IR spectral analysis combined with multivariate analysis of whole-cell extracts from ginseng leaves can be applied as a high-throughput discrimination system of cultivation ages and cultivars, a total of total 480 leaf samples belonging to 12 categories corresponding to four different cultivars (Yunpung, Kumpung, Chunpung, and an open-pollinated variety) and three different cultivation ages (1 yr, 2 yr, and 3 yr) were subjected to FT-IR. The spectral data were analyzed by principal component analysis and partial least squares-discriminant analysis. A dendrogram based on hierarchical clustering analysis of the FT-IR spectral data on ginseng leaves showed that leaf samples were initially segregated into three groups in a cultivation age-dependent manner. Then, within the same cultivation age group, leaf samples were clustered into four subgroups in a cultivar-dependent manner. The overall prediction accuracy for discrimination of cultivars and cultivation ages was 94.8% in a cross-validation test. These results clearly show that the FT-IR spectra combined with multivariate analysis from ginseng leaves can be applied as an alternative tool for discriminating of ginseng cultivars and cultivation ages. Therefore, we suggest that this result could be used as a rapid and reliable F1 hybrid seed-screening tool for accelerating the conventional breeding of ginseng.

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Section: Discussionmentioning

confidence: 99%

Discrimination of cultivation ages and cultivars of ginseng leaves using Fourier transform infrared spectroscopy combined with multivariate analysis

Kwon¹,

Ahn²,

Park³

et al. 2014

Journal of Ginseng Research

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“…Abundant fragment ions by multi-stage MS and high mass accuracy by TOF-MS offer structural information and the possibility of identifying unknown components. In NMR-based fingerprinting, the 1 H NMR spectrum provides a wealth of chemical information on ginseng 212, 238. NMR has the highest reliability in metabolomics 118.…”

Section: Applications and Challengesmentioning

confidence: 99%

Isolation and analysis of ginseng: advances and challenges

2011

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Ginseng occupies a prominent position in the list of best-selling natural products in the world. Because of its complex constituents, multidisciplinary techniques are needed to validate the analytical methods that support ginseng's use worldwide. In the past decade, rapid development of technology has advanced many aspects of ginseng research. The aim of this review is to illustrate the recent advances in the isolation and analysis of ginseng, and to highlight their new applications and challenges. Emphasis is placed on recent trends and emerging techniques. The current article reviews

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“…Several studies have used NMR-based methods on plant material, demonstrating biochemical differences according to tissue age, geographical location, genetic modification, and response to stress. For example, Shin et al (2007) performed fingerprinting analyses of fresh, differently old ginseng roots using 1 H-NMR spectroscopy and multivariate analysis techniques. The authors detected various distinct peaks in the 1 H-NMR spectra of the liquid-state ginseng roots.…”

Section: Suitability Of Other Spectroscopic Techniques For Root Analysesmentioning

confidence: 99%

Plant roots and spectroscopic methods – analyzing species, biomass and vitality

Rewald

Meinen

2013

Front. Plant Sci.

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In order to understand plant functioning, plant community composition, and terrestrial biogeochemistry, it is decisive to study standing root biomass, (fine) root dynamics, and interactions belowground. While most plant taxa can be identified by visual criteria aboveground, roots show less distinctive features. Furthermore, root systems of neighboring plants are rarely spatially segregated; thus, most soil horizons and samples hold roots of more than one species necessitating root sorting according to taxa. In the last decades, various approaches, ranging from anatomical and morphological analyses to differences in chemical composition and DNA sequencing were applied to discern species’ identity and biomass belowground. Among those methods, a variety of spectroscopic methods was used to detect differences in the chemical composition of roots. In this review, spectroscopic methods used to study root systems of herbaceous and woody species in excised samples or in situ will be discussed. In detail, techniques will be reviewed according to their usability to discern root taxa, to determine root vitality, and to quantify root biomass non-destructively or in soil cores holding mixtures of plant roots. In addition, spectroscopic methods which may be able to play an increasing role in future studies on root biomass and related traits are highlighted.

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Fingerprinting analysis of fresh ginseng roots of different ages using1H-NMR spectroscopy and principal components analysis

Cited by 44 publications

References 15 publications

Discrimination of cultivation ages and cultivars of ginseng leaves using Fourier transform infrared spectroscopy combined with multivariate analysis

Discrimination of cultivation ages and cultivars of ginseng leaves using Fourier transform infrared spectroscopy combined with multivariate analysis

Isolation and analysis of ginseng: advances and challenges

Plant roots and spectroscopic methods – analyzing species, biomass and vitality

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