Fingerprint Analysis and Identification of Strains ST309 as a Potential High Risk Clone in a Pseudomonas aeruginosa Population Isolated from Children with Bacteremia in Mexico City

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen and is associated with nosocomial infections. Its ability to thrive in a broad range of environments is due to a large and diverse genome of which its accessory genome is part. The objective of this study was to characterize P. aeruginosa strains isolated from children who developed bacteremia, using pulse-field gel electrophoresis, and in terms of its genomic islands, virulence genes, multilocus sequence type, and antimicrobial susceptibility. Our results showed that P. aeruginosa strains presented the seven virulence genes: toxA, lasB, lecA, algR, plcH, phzA1, and toxR, a type IV pilin alleles (TFP) group I or II. Additionally, we detected a novel pilin and accessory gene, expanding the number of TFP alleles to group VI. All strains presented the PAPI-2 Island and the majority were exoU+ and exoS+ genotype. Ten percent of the strains were multi-drug resistant phenotype, 18% extensively drug-resistant, 68% moderately resistant and only 3% were susceptible to all the antimicrobial tested. The most prevalent acquired β-Lactamase was KPC. We identified a group of ST309 strains, as a potential high risk clone. Our finding also showed that the strains isolated from patients with bacteremia have important virulence factors involved in colonization and dissemination as: a TFP group I or II; the presence of the exoU gene within the PAPI-2 island and the presence of the exoS gene.

Avaliação temporal do perfil de resistência de pseudomonas aeruginosa isoladas de pacientes internados em hospital oncológico em Belém-PA, Brasil em 2017

“…Especialistas da área apontam a P. aeruginosa como um patógeno corriqueiro entre pacientes críticos e cada vez mais cepas multirresistentes são isoladas em ambientes hospitalares 16,17,18,19 .…”

A Novel VIM-Type Metallo-β-Lactamase Variant, VIM-60, with Increased Hydrolyzing Activity against Fourth-Generation Cephalosporins in Pseudomonas aeruginosa Clinical Isolates in Japan

“…These isolates belonged to sequence type (ST) 1816 (allelic profile: 40, 5, 11, 3, 4, 40, 2) and were related to ST3002 (allelic profile: 40, 101, 11, 3, 4, 40, 2). P. aeruginosa ST1816 was first isolated in Mexico in 2011 (11). Until now, P. aeruginosa ST1816 was isolated in 2014 in Japan (id-2248 in P. aeruginosa MLST database [https://pubmlst.org/ paeruginosa/]).…”

Complete Genome Sequences of Four Extensively Drug-Resistant Pseudomonas aeruginosa Strains, Isolated from Adults with Ventilator-Associated Pneumonia at a Tertiary Referral Hospital in Mexico City

“…All of the strains were susceptible to polymyxin B. Two strains belong to sequence type 309 (ST-309), a high-risk clone previously documented in Mexico ( 3 ).…”