Abstract:Disruption methods used to extract proteins from the cell often require optimization in terms of yield increase and molecular integrity according to the cell type. Most cell lysis methods primarily target the cell wall. However, even for the wall-deficient strains, efficient extraction of molecules in or attached to membranous structures is a delicate process. In this study, we optimized the protein extraction technique for a cell wall deficient strain of Chlamydomonas reinhardtii, which is also a preferred ma… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.