1991
DOI: 10.1111/j.1365-2826.1991.tb00284.x
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Fine Structural Changes in Explants of the Neural Lobe of the Rat Hypophysis

Abstract: To obtain a purified population of pituicytes, pieces of rat neural lobes were dissected free of the pars intermedia, incubated in a variety of media, and fixed at regular intervals between 7 and 56 days of incubation. Many neurosecretory axons survived for up to 21 days without any apparent signs of degeneration. Most axons, however, degenerated and were progressively phagocytosed and subsequently eliminated by pituicytes and microglial cells. Lysed axons that were not eliminated, persisted as dense bodies or… Show more

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Cited by 10 publications
(6 citation statements)
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References 27 publications
(14 reference statements)
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“…earlv nostnatal (PN 1. PN3 and PN7), and ad& rats'as described previously ('Dellmann et al;. Briefly, the hypophyses were removed with the usual aseptic procedures and then placed in a small volume of Dulbecco's Modified Eagle's Medium (DMEM; GIBCO) containing 100 U/ml penicillin and 0.15 mg/ml streptomycin or in Gey's Balanced Salt Solutions.…”
Section: Methodsmentioning
confidence: 99%
“…earlv nostnatal (PN 1. PN3 and PN7), and ad& rats'as described previously ('Dellmann et al;. Briefly, the hypophyses were removed with the usual aseptic procedures and then placed in a small volume of Dulbecco's Modified Eagle's Medium (DMEM; GIBCO) containing 100 U/ml penicillin and 0.15 mg/ml streptomycin or in Gey's Balanced Salt Solutions.…”
Section: Methodsmentioning
confidence: 99%
“…Frequently, more or less short incubations of CVOs (4-8 days in vitro) were made in SSM (Olivieri-Sangiacomo and Correr, 1973). In more recent studies, cultures of explants from neural lobe were used to obtain long-term cultures (until 56 days) of pituicytes in SSM and in SFM conditions (Dellmann et al, 1991). Neurosecretory axons of the explants degenerated and were phagocytosed by pituicytes and microglial cells during the first 21 days in culture.…”
Section: Organotypic Culturesmentioning
confidence: 99%
“…Organotypic cultures of slices or pieces containing ventricular walls or CVOs were also developed to study the role of ependymal cells, e.g., in neuronependyma interactions (Dellmann et al, 1991;Goldman et al, 1993;Tominaga et al, 1994) or in diseases such as hydrocephalus linked to ''immotile cilia syndrome'' (Nakamura and Sato, 1993).…”
Section: Introductionmentioning
confidence: 99%
“…The intensity of stainings were also reduced. In addition, the accumulation of cell debris-like dots positive for vasopressin (15)(16)(17)(18). Thereafter, the stalk is reorganized into a neural lobe like tissue, but the cellular mechanism(s) responsible for such was seen in the median eminence and the ventral portions of the SON.…”
mentioning
confidence: 99%