Fine Needle Cytology and Flow Cytometry in Hashimoto Thyroiditis and Primary Thyroid Lymphoma

Zeppa, Pio; Vitale, Mario

doi:10.1159/000360067

Cited by 13 publications

(6 citation statements)

References 6 publications

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“…Boonyaarunnate et al demonstrated the occurrence of light chain restriction in nine (69.2%) of 13 PTLs [31]. However, light chain restriction has been reported in a minority of patients with Hashimoto's thyroiditis [36][37][38]. According to a study using fine needle aspiration materials, light chain restriction was observed in 75.0% of PTL cases [19].…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric, gene rearrangement, and karyotypic analyses of 110 cases of primary thyroid lymphoma: a single-institutional experience in Japan

et al. 2019

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Ancillary studies for primary nodal lymphomas have been well documented; however, studies of primary thyroid lymphoma (PTL) are limited. Here, we aimed to clarify the clinicopathological, flow cytometric, gene rearrangement, and karyotypic characteristics of PTL by investigation of a large series at a single institute. We performed flow cytometric, IgH rearrangement, and karyotypic analyses of 110 PTL tissues surgically resected at Kuma Hospital between January 2012 and April 2017. All PTLs were of B-cell origin, including mucosa-associated lymphoid tissue lymphoma (MALTL; 89 patients, 80.9%), diffuse large B-cell lymphoma (DLBCL; 18 patients, 16.4%), and follicular lymphoma (FL; three patients, 2.7%). In 96 (87.3%) patients, anti-thyroid antibodies were positive. For flow cytometry using aspirated and resected materials, light chain restriction was observed in 73.7% and 69.2% of examined cases, respectively. Heavy chain JH DNA rearrangement was observed in 65.4% of PTLs (58.1% of MALTL cases, 100% of DLBCL cases, and 100% of FL cases). Chromosomal abnormalities were detected in 49.0% of PTLs, and translocation was most frequently detected (24.0%), followed by addition (20.8%) and trisomy (18.8%). The most frequent (9.4%) karyotype was t(3;14)(q27;q32). Both FLs harbored t(14;18) (q32;q21), and the karyotype was not detected in patients with MALTL and DLBCL. The negative rate for all three examinations was 3.8%. We concluded that thyroid MALTL was cytogenetically different from that in other organs. Our results suggested that pre-operative flow cytometry analysis using aspirated materials was as reliable as that using resected materials.

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Section: Discussionmentioning

confidence: 99%

Flow cytometric, gene rearrangement, and karyotypic analyses of 110 cases of primary thyroid lymphoma: a single-institutional experience in Japan

et al. 2019

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“…However, some lymphomas do not satisfy this criterion [16,17]. Conversely, light chain restriction has been reported in a minority of patients with Hashimoto thyroiditis [18][19][20]. In addition, inadequate sampling for FNAC remains a limiting factor for performing FC [14].…”

Section: Discussionmentioning

confidence: 99%

Preoperative diagnostic algorithm of primary thyroid lymphoma using ultrasound, aspiration cytology, and flow cytometry

et al. 2017

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“…A skewed κ / λ ratio has been reported in HT and caution has been suggested in considering the imbalanced κ / λ ratio as diagnostic of lymphoma . Prominent clonal B‐cell populations have been described in reactive situations, particularly in cases in which follicle center derived B cells were involved, although they represent a rare finding .…”

Section: Discussionmentioning

confidence: 99%

Utilility of flow cytometry as ancillary study to improve the cytologic diagnosis of thyroid lymphomas

Stacchini

Pacchioni

Demurtas

et al. 2014

Cytometry Part B Clinical

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Results: A final diagnosis of lymphoma was given in 13 of 35 (37.1%) specimens. Among the 22 cases considered negative for lymphoma by FC, 11 were diagnosed as thyroiditis by cytology, 7 as reactive, 2 were anaplastic carcinoma, and 2 cases were considered cytologically suspicious for lymphoma but were not confirmed by further investigations. Histology on core biopsy or molecular analysis was available in 12 of 13 lymphoma cases (92.3%). Data obtained by the combination cytology/FC were confirmed in all cases on histology biopsies. Correlation with histology showed a sensitivity and a specificity of 100% for the combination cytology/FC. Conclusions: FC is an important additional test that can contribute with cytology to the identification of lymphomas of the thyroid. FC can detect the presence of small neoplastic lymphocyte populations and may contribute to the diagnosis of cases in which the lymphoid infiltrate is difficult to interpret on cytology alone. V C 2014 International Clinical Cytometry Society

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Fine Needle Cytology and Flow Cytometry in Hashimoto Thyroiditis and Primary Thyroid Lymphoma

Cited by 13 publications

References 6 publications

Flow cytometric, gene rearrangement, and karyotypic analyses of 110 cases of primary thyroid lymphoma: a single-institutional experience in Japan

Flow cytometric, gene rearrangement, and karyotypic analyses of 110 cases of primary thyroid lymphoma: a single-institutional experience in Japan

Preoperative diagnostic algorithm of primary thyroid lymphoma using ultrasound, aspiration cytology, and flow cytometry

Utilility of flow cytometry as ancillary study to improve the cytologic diagnosis of thyroid lymphomas

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