Fine-needle aspiration cytology combined with flow cytometry immunophenotyping is a rapid and accurate approach for the evaluation of suspicious superficial lymphoid lesions

Mathiot, Claire; Decaudin, Didier; Klijanienko, Jerzy; Couturier, Jérôme; Vincent‐Salomon, Anne; Dumont, J; Vielh, Philippe

doi:10.1002/dc.20487

Cited by 49 publications

(40 citation statements)

References 28 publications

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“…16,17 This argument is made stronger for patients in whom the site of disease is not easily accessible, such as the retroperitoneum, because diagnostic FNAC results can preclude the need for excisional biopsy. 18 Cytomorphologically, cases of metastatic squamous cell carcinoma showed sheets of polygonal cells with moderate amount of eosinophilic cytoplasm, high nucleocytoplasmic ratio and pleomorphic hyperchromatic nuclei with irregular nuclear borders against necrotic and lymphocytic background (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Role of FNAC in the diagnosis of cervical lymphadenopathy

Chandralekha¹,

Atla²

2017

Int J Res Med Sci

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Background: Cervical lymphadenopathy is one of the commonest presentations in inflammatory and neoplastic disorders. Fine Needle Aspiration Cytology (FNAC) is simple, quick, inexpensive and minimally invasive OPD technique used for establishing the etiology of cervical lymphadenopathy. In this study we describe cytomorphological patterns of cervical lymph nodes and its utility in establishing diagnosis. Objectives of present study were to assess the distribution of various cytomorphological patterns of cervical lymphadenopathy and to assess the age specific distribution of various cytomorphological patterns of cervical lymphadenopathy.Methods: This study was carried out in the Department of Pathology, Andhra Medical College on 200 cases of cervical lymphadenopathy over a period of three months from August - October 2017. FNAC diagnosis was correlated with relevant clinical findings and investigations.Results: Total 200 cases were studied. Of these, 170 (85%) were inflammatory and 30 (15%) were neoplastic. Reactive non-specific lymphadenitis was the most common disease found in 95 (47.5%) patients followed by tuberculous lymphadenitis in 60 patients (30%) and granulomatous lymphadenitis in 15 patients (7.5%). Among neoplastic lesions, metastatic tumours were reported in 26 patients (13%) and Lymphoproliferative disorder/Lymphoma was reported in 4 patients (2%). Highest incidence of cervical lymphadenopathy was found in patients of 10-39 years age group, among which most of the cases were non-specific lymphadenitis followed by tuberculous lymphadenitis. Amongst the neoplastic lesions, most of the cases were in the age group of 40-79 years.Conclusions: FNAC is simple, safe, reliable procedure for diagnosis of cervical lymphadenopathy.

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Section: Discussionmentioning

confidence: 99%

Role of FNAC in the diagnosis of cervical lymphadenopathy

Chandralekha¹,

Atla²

2017

Int J Res Med Sci

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“…Whereas breast may rarely be involved by leukemic and lymphoproliferative processes, the occurrence of intranodal lymph nodes and the rare sclerosing lobulitis widens the spectrum of unusual breast lymphoproliferative processes. FNA-FC has been used and described to accurately diagnose these unusual processes, avoiding demanding and useless biopsies, mainly in the case of secondary localizations of myeloid leukemia [63,64,65,66,67,68]. Whereas the application of FNA to the diagnosis of breast masses is generally declined, these applications still support the utility of its application.…”

Section: Other Extranodal Lymphoproliferative Processesmentioning

confidence: 99%

Extranodal Lymphoproliferative Processes and Flow Cytometry

Stacchini¹,

Demurtas²,

Aliberti³

2016

Acta Cytologica

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Objective: Fine-needle aspiration (FNA) cytology is a safe and cost-effective technique for the diagnosis of lymphoproliferative processes, especially when correlated with clinical and imaging studies. However, cytology alone may be unable to detect a lymphoid neoplastic process, as architectural features are less obvious than in histologic preparations and, in certain cases, reactive processes may mimic lymphoma. Flow cytometry (FC) has been recognized as an important ancillary technique in the diagnosis of lymphoid neoplasms and it can be used in conjunction with FNA in the evaluation of lymphoproliferative processes. Study Design: We performed a review of the published literature concerning FC applied to the detection of salivary glands and thyroid lymphoproliferative processes, which are frequently related to autoimmune diseases and difficult to diagnose by cytomorphology alone. Results: FC is able to detect and subtype non-Hodgkin lymphomas and may contribute to the exclusion of a neoplastic process in cytologically unclear cases. Conclusions: FC can be successfully applied in the differential diagnosis of lymphoproliferative processes in the head and neck region. The FNA-FC combined approach can reduce time to therapy and may prevent unnecessary surgical biopsies.

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“…In addition, the single cell suspension that is suitable for FCM analysis is easy to prepare (16). FCM is a rapid and sensitive multi-parameter analysis technique, which is important in the diagnosis of patients with lymph node enlargement (17). In the present study, a fine-needle aspiration biopsy of the lymph node was performed and FCM analyses were conducted.…”

mentioning

confidence: 98%

Secondary B-cell lymphoma diagnosed by fine-needle aspiration cytology and flow cytometry following penile carcinoma: A case report

Wang

Qiu

et al. 2016

Oncology Letters

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Abstract.The number of studies reporting lymphoma as a secondary tumor has gradually increased. However, few studies have reported that occurrence of lymphoma as a secondary tumor following treatment for penile carcinoma, particularly cases in which the lymphoma was diagnosed by fine-needle aspiration cytology and flow cytometry. The present study reports the case of a 62-year-old male patient who was troubled with frequent urination and repeated chest tightness for 5 years. The diagnosis upon admission was penile carcinoma. Two months subsequent to the tumor removal surgery, enlarged lymph nodes were extracted from the patient using fine-needle biopsy, to be analyzed using light microscopy and flow cytometry. Smear results indicated a large number of abnormal cells scattered in the right axillary lymph node. Flow cytometry immunophenotyping of fine-needle aspiration samples indicated the increased expression of cluster of differentiation (CD)79a, CD19, CD20, CD38, κ chain and human leukocyte antigen-DR, which supported a diagnosis of B-cell lymphoma. Thus, the patient was diagnosed with B-cell lymphoma based on the results of the fine-needle aspiration biopsy and flow cytometry. The method of diagnosis and causes of therapy-related leukemia are discussed in the present report.

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Fine-needle aspiration cytology combined with flow cytometry immunophenotyping is a rapid and accurate approach for the evaluation of suspicious superficial lymphoid lesions

Cited by 49 publications

References 28 publications

Role of FNAC in the diagnosis of cervical lymphadenopathy

Role of FNAC in the diagnosis of cervical lymphadenopathy

Extranodal Lymphoproliferative Processes and Flow Cytometry

Secondary B-cell lymphoma diagnosed by fine-needle aspiration cytology and flow cytometry following penile carcinoma: A case report

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