Fine‐Needle Aspiration Biopsy Diagnosis of Endodermal Sinus Tumor: Histologic and Ultrastructural Correlations

Abstract

Fine-needle aspiration biopsies from five patients with endodermal sinus tumors (ESTs) were reviewed, and the findings were correlated with histologic and ultrastructural appearances. In the aspiration smears, two types of tumor cells were seen, forming clusters of variable sizes. Type A cells had distinct cell borders, and their cytoplasm contained only occasional vacuoles. Type B cells had ill-defined cell borders and formed syncytial clusters; their cytoplasm was characterized by large numbers of rounded vacuoles. The background contained patches of mucoid material and macrophages with foamy cytoplasm. Eosinophilic hyaline cytoplasmic bodies and irregular deposits of intercellular basement membrane-like material were recognized easily in aspiration smears. These features correlated well with histologic and ultrastructural appearances. The significance of these findings in the fine-needle aspiration biopsy diagnosis of EST and its distinction from other germ-cell and non-germ-cell tumors is discussed.

Cytology of germ cell tumors

“…7 When GCTs are conIntracellular and extracellular hyaline globules that are sidered, YST most closely resembles EC but is said to PAS positive and diastase resistant and correspond to have somewhat less advanced degrees of cell clustera-fetoprotein production are a common histopathoing and nuclear abnormality. 18,19,24,27 Although based logic finding in YST and are often noted in cytologic on a limited number of cases, our experience undermaterial as well. 4 -8,18,19,24 They can appear to be either scores the difficulty of this differential diagnostic disintracellular or extracellular.…”

“…18,19,24 The cytoplasmic vacuoles of YST are typically Among non-GCT malignancies, the most serious bubbly in appearance and do not resemble the larger, differential diagnostic difficulty with YST is adenocar-''punched-out'' vacuoles of SD. 16,19,24 Schiller-Duval cinoma, which it resembles strongly. 4,27 Although imbodies are a frequent feature of YST histology, but they munohistochemical and historical findings may be exare generally not appreciated in cytologic samples, inploited in this regard, study of histologic material may cluding cell blocks prepared from body fluids.…”

“…4,27 Although imbodies are a frequent feature of YST histology, but they munohistochemical and historical findings may be exare generally not appreciated in cytologic samples, inploited in this regard, study of histologic material may cluding cell blocks prepared from body fluids. 5,7,8,19,24 be required for final diagnosis. 7 When GCTs are conIntracellular and extracellular hyaline globules that are sidered, YST most closely resembles EC but is said to PAS positive and diastase resistant and correspond to have somewhat less advanced degrees of cell clustera-fetoprotein production are a common histopathoing and nuclear abnormality.…”

“…Tumor types represented in this group included SD (n applied to extragonadal primary lesions. 15,16,18,19,[22][23][24][25][26][27][28][29][30] In this study, we reviewed a multi-institutional ex-Å 7) and EC (n Å 3) as well as single examples of YST, metastatic mixed GCT, and a peripheral neuroectoderperience with the cytologic diagnosis of GCT. The focus of our study was extracranial, extragonadal tumal tumor that arose in a teratoma.…”

Fine-Needle Aspiration Biopsy (FNAB) Diagnosis of Germ Cell Tumors: Histologic and Cytologic Correlations

“…Fine-needle aspiration biopsy findings in yolk sac tumors have been described in several studies. [2][3][4][5][22][23][24][25][26][27] The aspiration smears are moderately cellular, and contain tightly arranged clusters of epithelial cells with relatively abundant cytoplasm. These cells may be arranged as solid sheets, papillary or glandular structures, or as small globular structures (tumor balls) ( Figure 9).…”