Fine mapping of the R SC14Q locus for resistance to soybean mosaic virus in soybean

Abstract

Soybean mosaic virus (SMV) disease is one of the most destructive soybean (Glycine max (L.) Merr.) diseases worldwide, causing yield losses and significant deterioration in seed quality. 'Qihuang No.1' has a wide resistance spectrum and is extensively used in breeding programs in China. The dominant SMV resistance gene R SC14Q from the cultivar Qihuang No.1 was previously mapped to a 4.2 cM region on soybean chromosome 13 (formerly linkage group (LG) F). Fine mapping of R SC14Q is essential for high efficiency of marker-assisted selection (MAS) and its map-based cloning. Residual heterozygous lines (RHLs) derived from a soybean recombinant inbred line (RIL) were used to fine map R SC14Q . The RILs were obtained from a cross between 'Qihuang No.1' (resistant) and 'Nannong1138-2' (susceptible). The genomic sequence in an approximately 6 cM interval between the markers Satt334 and Sct_033 flanking R SC14Q was used to develop five simple sequence repeats and two InDel markers. Linkage analysis between inoculation phenotype and genetic markers localized R SC14Q to the interval between Satt334 and MY750, with genetic distances of 0.6 and 0.5 cM, respectively, corresponding to a physical distance on the 'Williams 82' draft assembly (Glyma1.13) of 1.18 Mb. Based on the genes sequences in the R SC14Q locus region with predicted functions for resistance, one SNP marker, MY525, was developed between Satt334 and MY750. Thus, the genomic region containing the R SC14Q was further narrowed to a 616 kb interval.

Detection and fine‐mapping of SC7 resistance genes via linkage and association analysis in soybean

“…Three independent, single‐dominant SMV resistance gene loci ( Rsv1 , Rsv3 , Rsv4 ) have been mapped on soybean chromosomes 13, 14, and 2, respectively (Yu et al ; Hayes et al ; Hayes and Saghai Maroof 2000; Gore et al ; Jeong and Saghai Maroof ; Saghai Maroof et al ). In China, several SMV resistance genes have been localized to chromosomes 2, 13, 14, and 6 (Fu et al ; Li et al ; Ma et al ; Wang et al , ; Yang and Gai ; Yang et al ).…”

The Potyviral P3 Protein Targets Eukaryotic Elongation Factor 1A to Promote the Unfolded Protein Response and Viral Pathogenesis

“…The microRNA and small interfering RNA pathways are also known to regulate defense against SMV . Resistance to the strains from China is derived from the Rsc loci, and these have been mapped to chromosomes 2, 13, and 14 in the cultivars Kefeng 1, Qihuang, and Dabaima, respectively Ma et al, 2011;Zheng et al, 2014).…”

Characterization of broad‐spectrum resistance to Soybean mosaic virus in soybean [Glycine max (L.) Merr.] cultivar ‘RN‐9’

“…Genetic linkage maps of SMV resistance loci for SC10, SC14, SC15 and SC18; (a) the linkage map of soybean linkage group C2 (chromosome 6) (Song et al. ); (b) the integrated linkage map of Rsc14‐r , Rsc15 , Rsc10‐r and Rsc18‐r with the RIL population from RN‐9 × 7605; (c) the linkage map of the resistance gene for SC14 in Qihuang No.1 (Ma et al., ); (d) the linkage map of the resistance gene for SC10 in Kefeng No.1 (Li et al., ); (e and f) the linkage maps of the resistance genes for SC18 in Kefeng No.1 and Qihuang 22, respectively (Li et al., ) [Colour figure can be viewed at wileyonlinelibrary.com]…”

“…Soybean mosaic virus strains (SC1‐SC21) used in this study were provided by the National Center for Soybean Improvement (NCSI, Nanjing, China) and maintained in leaves of susceptible cultivar ‘Nannong 1138‐2’ (NN). SMV strains SC10, SC14, SC15 and SC18 were identical with those previously used (Li et al., , ; Ma et al., ; Rui et al., ; Yang & Gai, ). The SMV inoculation and serological determination (double antibody sandwich enzyme‐linked immunosorbent assay, DAS‐ELISA) was conducted (Gao et al., ; Li et al., ) following the manufacturer's instructions (ACD Inc., Fayetteville, AR, USA).…”

“…In China, genes conferring resistance to multiple SMV strains in ‘Kefeng No.1’ (KF) were clustered in one region on chromosome 2 (linkage group, LG‐D1b). (Fu, Zhan, Zhi, Gai, & Yu, ; Li et al., , ; Wang, Ma, Yang et al., ; Wang, Zhi, Guo et al., 2004); SMV resistance genes identified from ‘Qihuang No.1’ (QH) and ‘Qihuang 22’ were mapped on chromosome 13 (LG‐F) (Li et al., ; Ma, Li, Wang, Liu, & Zhi, ; Ma et al., ; Zheng et al., ); strain‐specific resistance genes in ‘RN‐9’ ( Rsc15 ) and Dabaima ( Rsc4 ) were identified on chromosome 6 (LG‐C2) and 14 (LG‐B2), respectively (Rui et al., ; Wang, Ma, Liu et al., ; Yang & Gai, ). In addition, several major quantitative trait loci (QTLs) conferring soybean resistance to SMV have been detected on chromosome 6, 10 and 13 etc.…”