Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing

Fu, Fuyou; Liu, Xunjia; Wang, Rui; Zhai, Chun; Peng, Gary; Yu, Fengqun; Fernando, W. G. Dilantha

doi:10.1038/s41598-019-51191-z

Cited by 24 publications

(19 citation statements)

References 65 publications

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“…The Brassica genus includes important oil crops and vegetables 15 with yellow flower color as the most common form, while there are other colors, such as pale yellow, white, orange, and tangerine [16][17][18][19][20][21][22] . Compared with the studies of other traits in Brassica genus, such as yield [23][24][25][26][27][28] , fertility [29][30][31][32] , disease resistance [33][34][35] , the genetic studies of flower colors have been conducted earlier 16,17,36 . However, only the molecular mechanisms of white flower formation were understood [11][12][13][14]37,38 .…”

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Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.)

Zhang

Chen

et al. 2020

Sci Rep

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Flower color can be applied to landscaping and identification of the purity of seeds in hybrid production. However, the molecular basis of white flower trait remains largely unknown in Brassica rapa. in this study, an f 2 population was constructed from the cross between 15S1040 (white flower) and 92S105 (yellow flower) for fine mapping of white flower genes in B. rapa. Genetic analysis indicated that white flower trait is controlled by two recessive loci, Brwf1 and Brwf2. Using InDel and SNP markers, Brwf1 was mapped to a 49.6-kb region on chromosome A01 containing 9 annotated genes, and among them, Bra013602 encodes a plastid-lipid associated protein (PAP); Brwf2 was located in a 59.3-kb interval on chromosome A09 harboring 12 annotated genes, in which Bra031539 was annotated as a carotenoid isomerase gene (CRTISO). The amino acid sequences of BrPAP and BrCRTISO were compared between two yellow-flowered and three white-flowered lines and critical amino acid mutations of BrPAP and BrCRTISO were identified between yellow-flowered and white-flowered lines. Therefore, Bra013602 and Bra031539 were predicted as potential candidates for white flower trait. Our results provide a foundation for further identification of Brwf and increase understanding of the molecular mechanisms underlying white flower formation in Chinese cabbage. Preliminary mapping of the Brwf genes. To determine the locations of genes controlling white flower Scientific RepoRtS | (2020) 10:6080 | https://doi.

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Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.)

Zhang

Chen

et al. 2020

Sci Rep

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“…Using BSR-Seq, an R gene for resistance against Blackleg, Rlm1, was fine mapped in the B. napus cultivar “Quinta”, and a candidate gene was identified, BnA07G27460D, that encodes a serine/threonine protein kinase. This gene is homologous to the protein kinase STN7 in B. rapa , B. oleracea and A. thaliana , which is involved in systemic plant immune responses by regulating reactive oxygen species (ROS)-induced cell signalling at the thylakoid membrane [ 97 ]. BSR-Seq has also been applied in characterising Clubroot resistance in some Brassica speciesl; for example, in B. oleracea , the first Clubroot major R gene ( Rcr7 ) in the B. oleracea cultivar “Tekila” was identified; Bo7g108760 was the candidate TNL gene [ 98 ].…”

Section: Application Of Omics Technologies In Brassica mentioning

confidence: 99%

“…Furthermore, integrative approaches such as associative transcriptomics (AT) [ 114 ] and bulked RNA sequencing (BSR-Seq) [ 97 ] have allowed the incorporation of transcriptome data with genome-wide marker information to increase the power of detection for genomic loci controlling resistance or susceptibility in the host and virulence in the pathogen. These approaches have facilitated a fast-tracked identification of candidate genes underlying these loci and greatly facilitated the dissection of gene expression patterns during pathogen attack in important Brassica crops.…”

Section: Application Of Omics Technologies In Brassica mentioning

confidence: 99%

Understanding Host–Pathogen Interactions in Brassica napus in the Omics Era

Neik

Amas

Barbetti

et al. 2020

Plants

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Brassica napus (canola/oilseed rape/rapeseed) is an economically important crop, mostly found in temperate and sub-tropical regions, that is cultivated widely for its edible oil. Major diseases of Brassica crops such as Blackleg, Clubroot, Sclerotinia Stem Rot, Downy Mildew, Alternaria Leaf Spot and White Rust have caused significant yield and economic losses in rapeseed-producing countries worldwide, exacerbated by global climate change, and, if not remedied effectively, will threaten global food security. To gain further insights into the host–pathogen interactions in relation to Brassica diseases, it is critical that we review current knowledge in this area and discuss how omics technologies can offer promising results and help to push boundaries in our understanding of the resistance mechanisms. Omics technologies, such as genomics, proteomics, transcriptomics and metabolomics approaches, allow us to understand the host and pathogen, as well as the interaction between the two species at a deeper level. With these integrated data in multi-omics and systems biology, we are able to breed high-quality disease-resistant Brassica crops in a more holistic, targeted and accurate way.

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“…As the current major market for canola crops are food, feed, and biofuel industries [ 17 ], the improvement targets prioritize oil yield and quality [ 9 , 36 ], and further reduction of GSL content and other antinutritional compounds [ 104 ], while simultaneously enhancing resistance against the major pathogens and tolerance to abiotic stress and herbicides [ 31 , 69 , 116 , 117 , 132 , 134 , 148 , 173 , 174 , 175 ]. The approval for commercial release of DHA canola [ 86 , 87 ] is the most recent achievement in canola improvement and exploits natural resources in fatty acid biosynthesis, where modern genetic and gene technologies play a crucial role in the success.…”

Section: Prospects and Future Directionsmentioning

confidence: 99%

The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Ton

Neik

Batley

2020

Genes

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Since their domestication, Brassica oilseed species have undergone progressive transformation allied with the development of breeding and molecular technologies. The canola (Brassica napus) crop has rapidly expanded globally in the last 30 years with intensive innovations in canola varieties, providing for a wider range of markets apart from the food industry. The breeding efforts of B. napus, the main source of canola oil and canola meal, have been mainly focused on improving seed yield, oil quality, and meal quality along with disease resistance, abiotic stress tolerance, and herbicide resistance. The revolution in genetics and gene technologies, including genetic mapping, molecular markers, genomic tools, and gene technology, especially gene editing tools, has allowed an understanding of the complex genetic makeup and gene functions in the major bioprocesses of the Brassicales, especially Brassica oil crops. Here, we provide an overview on the contributions of these technologies in improving the major traits of B. napus and discuss their potential use to accomplish new improvement targets.

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Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing

Cited by 24 publications

References 65 publications

Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.)

Fine mapping and candidate gene analysis of the white flower gene Brwf in Chinese cabbage (Brassica rapa L.)

Understanding Host–Pathogen Interactions in Brassica napus in the Omics Era

The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

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