The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Ton, Linh Bao; Neik, Ting Xiang; Batley, Jacqueline

doi:10.3390/genes11101161

Cited by 23 publications

(18 citation statements)

References 161 publications

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“…Brassica napus (AACC, 2 n = 38), formed by hybridization between the diploid ancestors of B. rapa (AA, 2 n = 20) and B. oleracea (CC, 2 n = 18), is widely cultivated worldwide as both an oilseed and a vegetable ( Fujii and Ohmido, 2011 ; Ton et al, 2020 ). The purple leaf trait and the mechanisms underlying the anthocyanin accumulation has been widely documented in B. rapa and B. oleracea ( Zhang et al, 2014 and, 2015 ; He et al, 2016 ; Xie et al, 2016 ; Song et al, 2018 ; Guo et al, 2019 ; Yan et al, 2019 ); however, few studies have investigated Brassica napus anthocyanin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Zhang

et al. 2021

Front. Genet.

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Anthocyanins contribute to most colors of plants and play protective roles in response to abiotic stresses. Brassica napus is widely cultivated worldwide as both an oilseed and a vegetable. However, only several high anthocyanin-containing cultivars have been reported, and the mechanisms of anthocyanin accumulation have not been well-elucidated in B. napus. Here, the phenotype, comparative whole-genome identification, and gene expression analysis were performed to investigate the dynamic change of the anthocyanin content and the gene expression patterns of anthocyanin biosynthetic genes (ABGs) in B. napus. A total of 152 ABGs were identified in the B. napus reference genome. To screen out the critical genes involved in anthocyanin biosynthesis and accumulation, the RNA-seq of young leaves of two B. napus lines with purple leaves (PL) or green leaves (GL), and their F1 progeny at 41, 91, and 101 days were performed to identify the differentially expressed genes. The comparative expression analysis of these ABGs indicated that the upregulation of TT8 together with its target genes (such as DFR, ANS, UFGT, and TT19) might promote the anthocyanin accumulation in PL at the early developmental stage (41–91 days). While the downregulation of those ABGs and anthocyanin degradation at the late developmental stage (91–101 days) might result in the decrease in anthocyanin accumulation. Our results would enhance the understanding of the regulatory network of anthocyanin dynamic accumulation in B. napus.

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Section: Introductionmentioning

confidence: 99%

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Zhang

et al. 2021

Front. Genet.

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“…The use of phylogenetic tools has permitted many genera and species to be grouped according to conventional taxonomic techniques. A phylogenetic tree was created using the Mega X program and the findings suggested that the origin was diverse [ 20 , 45 , 46 ]. Sequences from a gene bank of different species were a dominant method of classification [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Morphological Formation, Fatty Acid Profile, and Molecular Identification of Some Landraces of Ethiopian Brassica as a Promising Crop to Support Breeding Programs

Khalaf

Al-Aziz

Ali

et al. 2021

Plants

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There has been an increased interest in oilseed crops for agro-industry research and development breeding programs to secure sustainable food and agriculture. The introgression of exotic genotypes of oilseed Brassica into cultivated relatives is inevitable in the genetic improvement of oilseed crops. This experimental attempt aimed to characterize the morphological and molecular basis for the identification and characterization of some Brassica genotypes. Fatty acid profile, yield, and morphology are under genetic control and can be used to identify genotypes. Characterization and identification were fulfilled for five accessions from Brassica spp. Plant height, height of first branch, number of branches and pods per plant, seed yield per plant, average pod length, number of seeds per pod, protein and oil contents (%), and fatty acid profile were examined. Besides, the relationship between seed yield and seed yield-contributing characteristics was estimated, as well as the phylogenetic relationship of the internal transcribed spacer (ITS). The genotypes varied significantly for all examined traits, taking into account the most important traits: seed yield per plant and oil content. For example, oil content in the samples ranged between 41.1 and 49.3%. Path analysis results showed a high and positive direct effect between each number of primary branches and the number of pods per plant with seed yield per plant (0.48). The morphological and molecular observations suggest that the Fay1, Fay3, Fay4, and Fay6 accessions belong to Brassica rapa, while Fay2 belongs to Brassica carinata. It can be concluded based on the present findings that the Fay3 genotype with the highest oil content and the lowest erucic acid content compared to the other genotypes can be proposed as a potential donor for future breeding programs for oil production and quality, while Fay1 can be utilized as donor to increase the seed yield per plant.

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“…Rapeseed oil obtained from B. napus seed is different from linoleic acid and linolenic acid, as oleic acid is relatively stable and has the valuable characteristic of reducing blood cholestrol. 48 Epidemiological studies have shown that residents in Mediterranean countries live longer and have lower rates of cardiovascular disease and breast cancer, which are closely related to the widespread consumption of olive oil rich in oleic acid. 49 Therefore, reducing the content of polyunsaturated fatty acid in edible rapeseed oil and increasing the content of oleic acid are some of the important goals of rapeseed quality breeding.…”

Section: ■ Discussionmentioning

confidence: 99%

Characteristics of Metabolites by Seed-Specific Inhibition of FAD2 in Brassica napus L

Zhou

Pan

Peng

et al. 2021

J. Agric. Food Chem.

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Fatty acid desaturase-2 (FAD2) is a key enzyme in the production of polyunsaturated fatty acids in plants. RNAi technology can reduce the expression of FAD2 genes in Brassica napus seeds and acquire transgenic B. napus plants with a high oleic acid content, but the effect of seed-specific inhibition of FAD2 expression on B. napus seed metabolites is not clear. Here we use widely targeted metabolomics to investigate the metabolites of normal-oleic-acid rapeseed (OA) and high-oleic-acid rapeseed (HOA) seeds, resulting in a total of 726 metabolites being detected. Among them, 24 differential metabolites were significantly downregulated and 88 differential metabolites were significantly upregulated in HOA rapeseed. In further lipid profile experiments, more lipids in B. napus seeds were accurately quantified. The contents of glycolipids and phospholipids that contain C18:1 increased significantly and C18:2 decreased because FAD2 expression was inhibited. The changes in the expression of key genes in related pathways were also consistent with the changes in metabolites. The insertion site of the ihpRNA plant expression vector was reconfirmed through genomewide resequencing, and the transgenic event did not change the sequence of FAD2 genes. There was no significant difference in the germination rate and germination potential between OA and HOA rapeseed seeds because the seedspecific ihpRNA plant expression vector did not affect other stages of plant growth. This work provides a theoretical and practical guidance for subsequent molecular breeding of high OA B. napus.

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The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Cited by 23 publications

References 161 publications

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Morphological Formation, Fatty Acid Profile, and Molecular Identification of Some Landraces of Ethiopian Brassica as a Promising Crop to Support Breeding Programs

Characteristics of Metabolites by Seed-Specific Inhibition of FAD2 in Brassica napus L

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