Finasteride Enhances Stem Cell Signals of Human Dermal Papilla Cells

Abstract

Background/Aim: Finasteride (FN) has been widely used to treat androgenetic alopecia (AGA). This study aimed at exploring the effect of FN on DP stem cell properties. Materials and Methods: Effect of FN on stem cell properties was tested in a DP cell line and 2 human primary DP cells (HDPCs1 and HDPCs2). Cell toxicity and growth were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. The aggregation behavior was observed by phase-contrast microscopy and a scanning electron microscope (SEM). Effects of FN on cell signaling were determined by western blotting and immunocytochemistry. Results: Treatment of DPCs with FN was able to significantly increase their aggregation behavior and the expression of stem cell transcription factors Nanog and Sox-2, when compared to the non-treated control. FN up-regulated stem cell regulatory proteins through the activation of protein kinase B (AKT), β-catenin, and integrin-β1. Conclusion: FN had an interesting biological effect on stem cell induction. These findings support the use of this drug for hair loss control and the development of regeneration approaches.

Pomegranate Peel Extract as an Inhibitor of SARS-CoV-2 Spike Binding to Human ACE2 Receptor (in vitro): A Promising Source of Novel Antiviral Drugs

“…As shown in Figure 5 , PPE at 0.04mg/mL reduced the activity of the 5α-reductase by 65% in HFDPC, after stimulation by testosterone. This effect was similar to that obtained by finasteride, used as a positive control (Rattanachitthawat et al, 2019 ).…”

A pomegranate peel extract as inhibitor of SARS-CoV-2 Spike binding to human ACE2 (in vitro): a promising source of novel antiviral drugs

“…As shown in Figure 5 , PPE at 0.04mg/mL reduced the activity of the 5α-Reductase by 65% in Human Follicle Dermal Papilla cells (HFDPC), after stimulation by testosterone. This effect was similar to that obtained by finasteride, used as positive control 54 .…”

Plant cell culture extract of Cirsium eriophorum with skin pore refiner activity by modulating sebum production and inflammatory response

“…To investigate on the capacity of CeCCE to modulate sebum production in skin cells, the 5α‐R activity was first induced in human dermal fibroblasts by testosterone 100 nM. Then the cells were treated with CeCCE at two concentrations and with the compound (5α,17β)‐(1,1‐Dimethylethyl)‐3‐oxo‐4‐azaandrost‐1‐ene‐17‐carboxamide, a 5α‐R inhibitor, known as Finasteride (Rattanachitthawat, Pinkhien, Opanasopit, Ngawhirunpat, & Chanvorachote, 2019), as a positive control. Significant inhibition of the testosterone‐induced enzymatic activity was produced by CeCCE 0.002% (52.2%) and 0.01% (44.5%), even stronger than those obtained with finasteride (Figure 1).…”