Filtration Sterilization

Walsh, Stephen; Denyer, Stephen Paul

doi:10.1002/9781118425831.ch15e

Cited by 2 publications

(1 citation statement)

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“…The objective or application typically determines the pore size of the filter. These include mycoplasma removal, laboratory sterilization, media filtration, biological fluids, gases, solutions, and solvents 81,82 .…”

Section: The Porosity Analysis Of Bc/kh Bc/kh-nis Bc/rhiz and Bc/rhiz...mentioning

confidence: 99%

Production and characterization of bacterial cellulose by Rhizobium sp. isolated from bean root

Almihyawi,

Musazade,

Alhussany

et al. 2024

Sci Rep

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Bacterial cellulose (BC) is a natural polymer renowned for its unique physicochemical and mechanical attributes, including notable water-holding capacity, crystallinity, and a pristine fiber network structure. While BC has broad applications spanning agriculture, industry, and medicine, its industrial utilization is hindered by production costs and yield limitations. In this study, Rhizobium sp. was isolated from bean roots and systematically assessed for BC synthesis under optimal conditions, with a comparative analysis against BC produced by Komagataeibacter hansenii. The study revealed that Rhizobium sp. exhibited optimal BC synthesis when supplied with a 1.5% glucose carbon source and a 0.15% yeast extract nitrogen source. Under static conditions at 30 °C and pH 6.5, the most favorable conditions for growth and BC production (2.5 g/L) were identified. Modifications were introduced using nisin to enhance BC properties, and the resulting BC-nisin composites were comprehensively characterized through various techniques, including FE-SEM, FTIR, porosity, swelling, filtration, and antibacterial activity assessments. The results demonstrated that BC produced by Rhizobium sp. displayed properties comparable to K. hansenii-produced BC. Furthermore, the BC-nisin composites exhibited remarkable inhibitory activity against Escherichia coli and Pseudomonas aeruginosa. This study contributes valuable insights into BC’s production, modification, and characterization utilizing Rhizobium sp., highlighting the exceptional properties that render it efficacious across diverse applications.

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Section: The Porosity Analysis Of Bc/kh Bc/kh-nis Bc/rhiz and Bc/rhiz...mentioning

confidence: 99%