Filtration and chemical treatment of waterborne pathogens

Sarma, Jyotirmoy

doi:10.1016/b978-0-12-818783-8.00006-2

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…The supernatant obtained is then typically clarified using membrane filtration to achieve optimal results during large‐volume filtration (Benjamin & Lawler, 2013; Doran, 2012; Todaro, 2014). The membranes have a fixed submicrometer pore structure with a uniform size distribution allowing the retention of particles larger than the maximum pore size of the filter (Sarma, 2020). Several types of membrane‐based processes have been developed for research and industrial purposes such as microfiltration (MF), ultrafiltration (UF), nanofiltration (NF), reverse osmosis (RO) and electrodialysis (ED), which rely on separating different solutes based on their size (MF, UF, NF), concentration (RO) or charge (ED), respectively (Batt & Tortorello, 2014).…”

Section: Introductionmentioning

confidence: 99%

Clarification of large-volume bacterial cultures using a centrifuge-free protocol

Allahghadry

Bojesen

Whitehead

et al. 2022

Journal of Applied Microbiology

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To provide a reliable, reproducible and centrifuge-free filtration protocol for clarification of large volumes of bacterial cultures. Methods and Results: Four experiments were designed to compare different techniques enabling clarification of Escherichia coli cultures using as a benchmark the concentration and quality of bacterial outer membrane vesicles (OMVs). The experiments were designed to examine the performance of different extraction methods on large volume (≥1 L) filtrations of bacterial culture media. Performance parameters included filtration flow rates, sterility testing and characterization of the filtrates by: (i) SDS-PAGE, (ii) cryogenic transmission electron microscopy, (iii) nanoparticle tracking analysis and (iv) Qubit protein quantification. The experiments revealed that: (i) addition of the filter aid Diatomaceous Earth to the bacterial cultures improved filtration flow rates significantly and eliminated the need for centrifugation prior to filtration; (ii) sterile filtration was successful as no bacterial passage was identified through the membrane filter; (iii) centrifuge-free filtrates contained an increased amount of OMVs compared to centrifuged filtrates. Conclusions:In comparison to conventional centrifuge-based protocols, the clarification method presented has universal applicability for a broad range of microbial extraction procedures, regardless of the volume of culture harvested. Moreover, the decreased amount of OMVs presented in the filtrates following centrifugation step provides an additional argument in favour of a centrifuge-free approach.Significance and Impact of the Study: Sterile filtration is a universal method for the clarification of bacterial cultures. Common challenges related to filtration include filter clogging and long processing times, due to limited centrifugation capacity, which can affect product quality. The proposed protocol is likely to ensure a highly effective filtration process and could be a novel approach in improving the filtrate products without the need of centrifugation.

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Section: Introductionmentioning

confidence: 99%

Clarification of large-volume bacterial cultures using a centrifuge-free protocol

Allahghadry

Bojesen

Whitehead

et al. 2022

Journal of Applied Microbiology

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“…Despite the efficacy of the current disinfection methods to control microbial pathogens, the resistance of some pathogenic microorganisms requires high chemical disinfectant dosage with formation of harmful disinfection by-products and even can be present in the user final application (point-of-use water) (Legay et al, 2010;Li et al, 2008;Stoquart et al, 2012). Microbial growth may occur during treatment, distribution, and within plumbing systems of final users with presence of biofilm with increased resistance to disinfection (Sarma, 2020). Waterborne infection can be a great risk at the time and point of supply and outbreaks are periodically reported in water relevant environments like hospitals among others (Blasco et al, 2008;Moore and Walker, 2014).…”

Section: Introductionmentioning

confidence: 99%

Natural antimicrobial compounds immobilised on silica microparticles as filtering materials: Impact on the metabolic activity and bacterial viability of waterborne microorganisms

Ribes

Ruiz-Rico

Moreno-Mesonero

et al. 2021

Environmental Technology & Innovation

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“…Worldwide groundwater is a very common source used for domestic, agricultural and industrial purposes due to its availability, quality and low cost of extraction (Carrard et al, 2019). Groundwater seems to be chemical and pathogenic contaminants free water which is considered as a valuable safe water source for its quality and quantity (Sarma, 2020). However, rapid urbanization, industrialization, advance economic growth, modern agriculture for food security, huge growth of population and climate change issues are changing the groundwater consumption pattern and create unparallel pressure on groundwater source (Assembly, 2012).…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Groundwater Quality in Arsenic and Salinity Prone Areas of Jashore, Bangladesh

Ahmed¹,

Islam²,

Hasan

et al. 2020

IJEEG

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The groundwater contamination by arsenic is a large-scale pollution in drinking water history. Safe water supply is a big challenge due to critical hydrogeological situation and water quality problems in this area. The analytical results show that a range of pH, TDS, chloride, total alkalinity, total hardness, sodium, potassium, calcium, magnesium, manganese, iron and arsenic were found between 7.50-7.23, 504.00-201.00 mg/L, 90.30-31.43 mg/L, 410.81-174.31mg/L, 616.47-202.97 mg/L, 52.59-13.28 mg/L, 17.13-2.87 mg/L, 108.57-44.53 mg/L, 83.87-22.29 mg/L, 1.78-0.01 mg/L, 11.78-1.45 mg/L, 0.42-0.02 mg/L, respectively. This study will help making a future plan for groundwater quality monitoring and its hydrogeological application for safe water source identification

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Filtration and chemical treatment of waterborne pathogens

Cited by 6 publications

References 17 publications

Clarification of large-volume bacterial cultures using a centrifuge-free protocol

Clarification of large-volume bacterial cultures using a centrifuge-free protocol

Natural antimicrobial compounds immobilised on silica microparticles as filtering materials: Impact on the metabolic activity and bacterial viability of waterborne microorganisms

Monitoring of Groundwater Quality in Arsenic and Salinity Prone Areas of Jashore, Bangladesh

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