Natural antimicrobial compounds immobilised on silica microparticles as filtering materials: Impact on the metabolic activity and bacterial viability of waterborne microorganisms

Immobilization of Natural Antimicrobial Compounds on Food-Grade Supports as a New Strategy to Preserve Fruit-Derived Foods

“…The biocidal properties of the immobilized antimicrobial are characterized by in vitro antimicrobial performance testing against microorganisms of interest in different microbial life stages [68]. These viability tests can be combined with microscopic and molecular techniques to reduce errors from viable but non-culturable (VBNC) microorganisms and to help to elucidate the mechanism of action of the grafted antimicrobial [69]. In situ antimicrobial testing is also needed to evaluate biocidal properties in real food matrices, the impact of the immobilized antimicrobial on food properties, and the potential leaching of the grafted biomolecule [70].…”

Natural antimicrobial-coated supports as filter aids for the microbiological stabilisation of drinks

“…However, the enzymatic activity was less affected by wine components when lysozyme was immobilised. due to natural antimicrobials covalently immobilised on their surfaces have recently been reported (Cappannella et al, 2016;Kroll et al, 2012;Ribes et al, 2020;Wang et al, 2017;Zhang et al, 2021). The most important parameter to be evaluated with these antimicrobial-coated filters is their removal capability and, consequently, their mode of action.…”

“…This represents the notion that covalent immobilisation prevents enzyme inactivation in the presence of wine components. Other studied coated filtering materials with different liquid matrices (water, beer, apple juice) are silica microparticles covalently coated with EOCs for depth filtration (Peña-Gómez et al, 2019a, 2019bRibes et al, 2020). These coated silica filters presented a removal capability of 4-5 LRVs against different microorganisms naturally present or inoculated in diverse matrices, and their removal capability was maintained after filtering multiple samples and pre-conditioning (washing with water) (Peña-Gómez et al, 2019b.…”

Sustainable Filters with Antimicrobial Action from Sugarcane Bagasse: A Novel Waste Utilization Approach

“…Therefore, in recent years, there has been an increasing interest in studying the use of functionalized materials for liquid food processing to reduce the number of microorganisms present. For instance, some authors have used silica-based filters with carvacrol, eugenol, thymol, or vanillin for the filtration of waterborne microorganisms from water [11], varying the silica microparticles size and filtration layer thickness, achieving 3 to 5 log reduction values for different microorganisms. Dikic et al developed composites using natural zeolite, thymol, and carvacrol through supercritical solvent impregnation; the materials showed antibacterial activity against E. coli and S. aureus in spring water and lake water [12].…”